Yeast RNA polymerase I: A eukaryotic zinc metalloenzyme

David S. Auld, Ikuo Atsuya, Carmen Campino, Pablo Valenzuela

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

46 Citas (Scopus)


Microwave excitation spectrometry and metal binding inhibition studies show that zinc is a catlytically essential component of the highly purified RNA polymerase I from yeast, the first eukaryotic RNA polymerase I available in quantities sufficient for such studies. It contains 2.4 g-atom of zinc based on a molecular weight of 6.5 × 105 (8). Copper, iron, manganese and magnesium are absent, i.e., below the limits of detection, 10-13 to 10-14 g-atoms. A number of derivatives of 1,10-phenanthroline reversibly inhibit the polymerase catalyzed reaction, apparently by forming a ternary polymerase·Zn·OP complex while the nonchelating isomer, 1,7-phenanthroline, is ineffective.

Idioma originalInglés
Páginas (desde-hasta)548-554
Número de páginas7
PublicaciónBiochemical and Biophysical Research Communications
EstadoPublicada - 22 mar. 1976

Áreas temáticas de ASJC Scopus

  • Biofísica
  • Bioquímica
  • Biología molecular
  • Biología celular


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