Xyloglucan fucosyltransferase, an enzyme involved in plant cell wall biosynthesis

Robyn M. Perrin, Amy E. DeRocher, Maor Bar-Peled, Weiqing Zeng, Lorena Norambuena, Ariel Orellana, Natasha V. Raikhel, Kenneth Keegstra

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

209 Citas (Scopus)

Resumen

Cell walls are crucial for development, signal transduction, and disease resistance in plants. Cell walls are made of cellulose, hemicelluloses, and pectins. Xyloglucan (XG), the principal load-bearing hemicellulose of dicotyledonous plants, has a terminal fucosyl residue. A 60-kilodalton fucosyltransferase (FTase) that adds this residue was purified from pea epicotyls. Peptide sequence information from the pea FTase allowed the cloning of a homologous gene, AtFT1, from Arabidopsis. Antibodies raised against recombinant AtFTase immunoprecipitate FTase enzyme activity from solubilized Arabidopsis membrane proteins, and AtFT1 expressed in mammalian COS cells results in the presence of XG FTase activity in these cells.

Idioma originalInglés
Páginas (desde-hasta)1976-1979
Número de páginas4
PublicaciónScience
Volumen284
N.º5422
DOI
EstadoPublicada - 18 jun 1999

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