WRKY7, -11 and -17 transcription factors are modulators of the bZIP28 branch of the unfolded protein response during PAMP-triggered immunity in Arabidopsis thaliana

Paulina Arraño-Salinas, José Domínguez-Figueroa, Ariel Herrera-Vásquez, Diego Zavala, Joaquin Medina, Jesús Vicente-Carbajosa, Claudio Meneses, Paulo Canessa, Adrián A. Moreno, Francisca Blanco-Herrera

Resultado de la investigación: Article

1 Cita (Scopus)

Resumen

Plants must defend themselves against pathogens. The defense response requires greater protein synthesis, which generates endoplasmic reticulum (ER) stress, yet failure to attenuate this stress has detrimental effects. WRKY7/11/17 transcription factors (TFs) are negative regulators of immunity since mutants are more resistant to Pseudomonas syringae pv tomato (Pst) infection. Here, we reveal a connection between ER-stress and the molecular mechanisms underlying the wrky mutant phenotype. The bZIP28 TF upregulates ER-chaperone expression (BiP1/2, ERdj3B, and SDF2) upon exposure of Arabidopsis to a bacterial defense elicitor, flagellin 22 (Flg22). Also, the activation of ER-chaperones is more sustained in double and triple wrky mutants treated with Flg22, suggesting that WRKY7/11/17 TFs downregulate these genes. Moreover, wrky mutants accumulate more bZIP28 transcripts in response to Flg22, indicating that WRKY7/11/17 transcriptionally repress this TF. Using Arabidopsis protoplasts, we also demonstrate that WRKYs bind to the bZIP28 promoter via W-box elements. Additionally, triple wrky mutants are more resistant, whilst bzip28 mutants are more susceptible, to Pst infection. Finally, we postulate a model of PAMP-Triggered Immunity regulation, where Flg22 activates bZIP28-signaling inducing the expression of ER-stress genes, as well as WRKY7/11/17 expression, which in turn inhibits PTI by downregulating bZIP28, controlling physiological responses in the Arabidopsis-Pst interaction.

Idioma originalEnglish
PublicaciónPlant Science
DOI
EstadoAccepted/In press - 1 ene 2018

Huella dactilar

unfolded protein response
Flagellin
Unfolded Protein Response
Pseudomonas syringae
Arabidopsis
Endoplasmic Reticulum Stress
Immunity
flagellin
Transcription Factors
Lycopersicon esculentum
endoplasmic reticulum
Arabidopsis thaliana
transcription factors
immunity
mutants
Endoplasmic Reticulum
Down-Regulation
tomatoes
Protoplasts
Infection

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Citar esto

@article{379c031f104441ed9a0370ac77fbe85c,
title = "WRKY7, -11 and -17 transcription factors are modulators of the bZIP28 branch of the unfolded protein response during PAMP-triggered immunity in Arabidopsis thaliana",
abstract = "Plants must defend themselves against pathogens. The defense response requires greater protein synthesis, which generates endoplasmic reticulum (ER) stress, yet failure to attenuate this stress has detrimental effects. WRKY7/11/17 transcription factors (TFs) are negative regulators of immunity since mutants are more resistant to Pseudomonas syringae pv tomato (Pst) infection. Here, we reveal a connection between ER-stress and the molecular mechanisms underlying the wrky mutant phenotype. The bZIP28 TF upregulates ER-chaperone expression (BiP1/2, ERdj3B, and SDF2) upon exposure of Arabidopsis to a bacterial defense elicitor, flagellin 22 (Flg22). Also, the activation of ER-chaperones is more sustained in double and triple wrky mutants treated with Flg22, suggesting that WRKY7/11/17 TFs downregulate these genes. Moreover, wrky mutants accumulate more bZIP28 transcripts in response to Flg22, indicating that WRKY7/11/17 transcriptionally repress this TF. Using Arabidopsis protoplasts, we also demonstrate that WRKYs bind to the bZIP28 promoter via W-box elements. Additionally, triple wrky mutants are more resistant, whilst bzip28 mutants are more susceptible, to Pst infection. Finally, we postulate a model of PAMP-Triggered Immunity regulation, where Flg22 activates bZIP28-signaling inducing the expression of ER-stress genes, as well as WRKY7/11/17 expression, which in turn inhibits PTI by downregulating bZIP28, controlling physiological responses in the Arabidopsis-Pst interaction.",
keywords = "Basal defense response, bZIP, Flg22, Unfolded protein response, WRKY",
author = "Paulina Arra{\~n}o-Salinas and Jos{\'e} Dom{\'i}nguez-Figueroa and Ariel Herrera-V{\'a}squez and Diego Zavala and Joaquin Medina and Jes{\'u}s Vicente-Carbajosa and Claudio Meneses and Paulo Canessa and Moreno, {Adri{\'a}n A.} and Francisca Blanco-Herrera",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.plantsci.2018.09.019",
language = "English",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",

}

WRKY7, -11 and -17 transcription factors are modulators of the bZIP28 branch of the unfolded protein response during PAMP-triggered immunity in Arabidopsis thaliana. / Arraño-Salinas, Paulina; Domínguez-Figueroa, José; Herrera-Vásquez, Ariel; Zavala, Diego; Medina, Joaquin; Vicente-Carbajosa, Jesús; Meneses, Claudio; Canessa, Paulo; Moreno, Adrián A.; Blanco-Herrera, Francisca.

En: Plant Science, 01.01.2018.

Resultado de la investigación: Article

TY - JOUR

T1 - WRKY7, -11 and -17 transcription factors are modulators of the bZIP28 branch of the unfolded protein response during PAMP-triggered immunity in Arabidopsis thaliana

AU - Arraño-Salinas, Paulina

AU - Domínguez-Figueroa, José

AU - Herrera-Vásquez, Ariel

AU - Zavala, Diego

AU - Medina, Joaquin

AU - Vicente-Carbajosa, Jesús

AU - Meneses, Claudio

AU - Canessa, Paulo

AU - Moreno, Adrián A.

AU - Blanco-Herrera, Francisca

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Plants must defend themselves against pathogens. The defense response requires greater protein synthesis, which generates endoplasmic reticulum (ER) stress, yet failure to attenuate this stress has detrimental effects. WRKY7/11/17 transcription factors (TFs) are negative regulators of immunity since mutants are more resistant to Pseudomonas syringae pv tomato (Pst) infection. Here, we reveal a connection between ER-stress and the molecular mechanisms underlying the wrky mutant phenotype. The bZIP28 TF upregulates ER-chaperone expression (BiP1/2, ERdj3B, and SDF2) upon exposure of Arabidopsis to a bacterial defense elicitor, flagellin 22 (Flg22). Also, the activation of ER-chaperones is more sustained in double and triple wrky mutants treated with Flg22, suggesting that WRKY7/11/17 TFs downregulate these genes. Moreover, wrky mutants accumulate more bZIP28 transcripts in response to Flg22, indicating that WRKY7/11/17 transcriptionally repress this TF. Using Arabidopsis protoplasts, we also demonstrate that WRKYs bind to the bZIP28 promoter via W-box elements. Additionally, triple wrky mutants are more resistant, whilst bzip28 mutants are more susceptible, to Pst infection. Finally, we postulate a model of PAMP-Triggered Immunity regulation, where Flg22 activates bZIP28-signaling inducing the expression of ER-stress genes, as well as WRKY7/11/17 expression, which in turn inhibits PTI by downregulating bZIP28, controlling physiological responses in the Arabidopsis-Pst interaction.

AB - Plants must defend themselves against pathogens. The defense response requires greater protein synthesis, which generates endoplasmic reticulum (ER) stress, yet failure to attenuate this stress has detrimental effects. WRKY7/11/17 transcription factors (TFs) are negative regulators of immunity since mutants are more resistant to Pseudomonas syringae pv tomato (Pst) infection. Here, we reveal a connection between ER-stress and the molecular mechanisms underlying the wrky mutant phenotype. The bZIP28 TF upregulates ER-chaperone expression (BiP1/2, ERdj3B, and SDF2) upon exposure of Arabidopsis to a bacterial defense elicitor, flagellin 22 (Flg22). Also, the activation of ER-chaperones is more sustained in double and triple wrky mutants treated with Flg22, suggesting that WRKY7/11/17 TFs downregulate these genes. Moreover, wrky mutants accumulate more bZIP28 transcripts in response to Flg22, indicating that WRKY7/11/17 transcriptionally repress this TF. Using Arabidopsis protoplasts, we also demonstrate that WRKYs bind to the bZIP28 promoter via W-box elements. Additionally, triple wrky mutants are more resistant, whilst bzip28 mutants are more susceptible, to Pst infection. Finally, we postulate a model of PAMP-Triggered Immunity regulation, where Flg22 activates bZIP28-signaling inducing the expression of ER-stress genes, as well as WRKY7/11/17 expression, which in turn inhibits PTI by downregulating bZIP28, controlling physiological responses in the Arabidopsis-Pst interaction.

KW - Basal defense response

KW - bZIP

KW - Flg22

KW - Unfolded protein response

KW - WRKY

UR - http://www.scopus.com/inward/record.url?scp=85054428412&partnerID=8YFLogxK

U2 - 10.1016/j.plantsci.2018.09.019

DO - 10.1016/j.plantsci.2018.09.019

M3 - Article

JO - Plant Science

JF - Plant Science

SN - 0168-9452

ER -