Variants in ABCG8 and TRAF3 genes confer risk for gallstone disease in admixed Latinos with Mapuche Native American ancestry

Bernabé I. Bustos, Eduardo Pérez-Palma, Stephan Buch, Lorena Azócar, Eleodoro Riveras, Giorgia D. Ugarte, Mohammad Toliat, Peter Nürnberg, Wolfgang Lieb, Andre Franke, Sebastian Hinz, Greta Burmeister, Witigo von Schönfels, Clemens Schafmayer, Henry Völzke, Uwe Völker, Georg Homuth, Markus M. Lerch, José Luis Santos, Klaus PuschelClaudia Bambs, Juan Carlos Roa, Rodrigo A. Gutiérrez, Jochen Hampe, Giancarlo V. De Ferrari, Juan Francisco Miquel

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3 Citas (Scopus)

Resumen

Latin Americans and Chilean Amerindians have the highest prevalence of gallstone disease (GSD) and gallbladder cancer (GBC) in the world. A handful of loci have been associated with GSD in populations of predominantly European ancestry, however, they only explain a small portion of the genetic component of the disease. Here, we performed a genome-wide association study (GWAS) for GSD in 1,095 admixed Chilean Latinos with Mapuche Native American ancestry. Disease status was assessed by cholecystectomy or abdominal ultrasonography. Top-10 candidate variants surpassing the suggestive cutoff of P < 1 × 10 −5 in the discovery cohort were genotyped in an independent replication sample composed of 1,643 individuals. Variants with positive replication were further examined in two European GSD populations and a Chilean GBC cohort. We consistently replicated the association of ABCG8 gene with GSD (rs11887534, P = 3.24 × 10 −8 , OR = 1.74) and identified TRAF3 (rs12882491, P = 1.11 × 10 −7 , OR = 1.40) as a novel candidate gene for the disease in admixed Chilean Latinos. ABCG8 and TRAF3 variants also conferred risk to GBC. Gene expression analyses indicated that TRAF3 was significantly decreased in gallbladder (P = 0.015) and duodenal mucosa (P = 0.001) of GSD individuals compared to healthy controls, where according to GTEx data in the small intestine, the presence of the risk allele contributes to the observed effect. We conclude that ABCG8 and TRAF3 genes are associated with GSD and GBC in admixed Latinos and that decreased TRAF3 levels could enhance gallbladder inflammation as is observed in GSD and GSD-associated GBC.

Idioma originalInglés
Número de artículo772
PublicaciónScientific Reports
Volumen9
N.º1
DOI
EstadoPublicada - 28 ene 2019

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    Bustos, B. I., Pérez-Palma, E., Buch, S., Azócar, L., Riveras, E., Ugarte, G. D., Toliat, M., Nürnberg, P., Lieb, W., Franke, A., Hinz, S., Burmeister, G., von Schönfels, W., Schafmayer, C., Völzke, H., Völker, U., Homuth, G., Lerch, M. M., Santos, J. L., ... Miquel, J. F. (2019). Variants in ABCG8 and TRAF3 genes confer risk for gallstone disease in admixed Latinos with Mapuche Native American ancestry. Scientific Reports, 9(1), [772]. https://doi.org/10.1038/s41598-018-35852-z