Utilidad de tinción de tricrómico de masson en la cuantificación de densidad media vascular en mucosa oral normal, displasia epitelial y carcinoma oral de células escamosas

Felipe Cáceres, Gilberto Herrera, Alejandra Fernández, Javier Fernández, René Martínez, Daniela Carvajal, Ziyad S. Haidar

Resultado de la investigación: Article

Resumen

The objective of this study was to evaluate the utility of Masson's Trichrome (TM) staining in the quantification of the mean vascular density (DMV) in samples of normal oral mucosa (MON), oral epithelial dysplasia (ODE) and oral squamous cell carcinoma (COCE). The design - a descriptive study of case series. We analyzed 17 samples of MON, 15 samples of DEO and 16 samples of COCE, stained with TM. To determine usefulness, we compared and analyzed the same samples, either stained with TM or with immunohistochemical technique against CD31. Quantification of the DMV was performed in the 3 areas of greatest vascularization in each sample. DMV was determined according to diagnosis by TM staining and immunohistochemistry against CD31, and the correlation between the two was then calculated. DMV quantified with TM and against CD31 differs according to the diagnosis, with an increase in DMV upon malignant diagnosis. No differences were found when comparing DMV quantified with TM and against CD31. The correlation of the DMV analyzed by TM and against CD31 is significant and moderate. Quantification of blood vessels is possible by TM staining in samples of MON, DEO and COCE. TM staining is moderately correlated with immunohistochemistry against CD31.

Idioma originalSpanish
Páginas (desde-hasta)1576-1581
Número de páginas6
PublicaciónInternational Journal of Morphology
Volumen35
N.º4
DOI
EstadoPublished - 1 dic 2017

Huella dactilar

Mouth Mucosa
Blood Vessels
Squamous Cell Carcinoma
Staining and Labeling
Immunohistochemistry
trichrome stain

ASJC Scopus subject areas

  • Anatomy

Citar esto

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title = "Utilidad de tinci{\'o}n de tricr{\'o}mico de masson en la cuantificaci{\'o}n de densidad media vascular en mucosa oral normal, displasia epitelial y carcinoma oral de c{\'e}lulas escamosas",
abstract = "The objective of this study was to evaluate the utility of Masson's Trichrome (TM) staining in the quantification of the mean vascular density (DMV) in samples of normal oral mucosa (MON), oral epithelial dysplasia (ODE) and oral squamous cell carcinoma (COCE). The design - a descriptive study of case series. We analyzed 17 samples of MON, 15 samples of DEO and 16 samples of COCE, stained with TM. To determine usefulness, we compared and analyzed the same samples, either stained with TM or with immunohistochemical technique against CD31. Quantification of the DMV was performed in the 3 areas of greatest vascularization in each sample. DMV was determined according to diagnosis by TM staining and immunohistochemistry against CD31, and the correlation between the two was then calculated. DMV quantified with TM and against CD31 differs according to the diagnosis, with an increase in DMV upon malignant diagnosis. No differences were found when comparing DMV quantified with TM and against CD31. The correlation of the DMV analyzed by TM and against CD31 is significant and moderate. Quantification of blood vessels is possible by TM staining in samples of MON, DEO and COCE. TM staining is moderately correlated with immunohistochemistry against CD31.",
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author = "Felipe C{\'a}ceres and Gilberto Herrera and Alejandra Fern{\'a}ndez and Javier Fern{\'a}ndez and Ren{\'e} Mart{\'i}nez and Daniela Carvajal and Haidar, {Ziyad S.}",
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Utilidad de tinción de tricrómico de masson en la cuantificación de densidad media vascular en mucosa oral normal, displasia epitelial y carcinoma oral de células escamosas. / Cáceres, Felipe; Herrera, Gilberto; Fernández, Alejandra; Fernández, Javier; Martínez, René; Carvajal, Daniela; Haidar, Ziyad S.

En: International Journal of Morphology, Vol. 35, N.º 4, 01.12.2017, p. 1576-1581.

Resultado de la investigación: Article

TY - JOUR

T1 - Utilidad de tinción de tricrómico de masson en la cuantificación de densidad media vascular en mucosa oral normal, displasia epitelial y carcinoma oral de células escamosas

AU - Cáceres, Felipe

AU - Herrera, Gilberto

AU - Fernández, Alejandra

AU - Fernández, Javier

AU - Martínez, René

AU - Carvajal, Daniela

AU - Haidar, Ziyad S.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - The objective of this study was to evaluate the utility of Masson's Trichrome (TM) staining in the quantification of the mean vascular density (DMV) in samples of normal oral mucosa (MON), oral epithelial dysplasia (ODE) and oral squamous cell carcinoma (COCE). The design - a descriptive study of case series. We analyzed 17 samples of MON, 15 samples of DEO and 16 samples of COCE, stained with TM. To determine usefulness, we compared and analyzed the same samples, either stained with TM or with immunohistochemical technique against CD31. Quantification of the DMV was performed in the 3 areas of greatest vascularization in each sample. DMV was determined according to diagnosis by TM staining and immunohistochemistry against CD31, and the correlation between the two was then calculated. DMV quantified with TM and against CD31 differs according to the diagnosis, with an increase in DMV upon malignant diagnosis. No differences were found when comparing DMV quantified with TM and against CD31. The correlation of the DMV analyzed by TM and against CD31 is significant and moderate. Quantification of blood vessels is possible by TM staining in samples of MON, DEO and COCE. TM staining is moderately correlated with immunohistochemistry against CD31.

AB - The objective of this study was to evaluate the utility of Masson's Trichrome (TM) staining in the quantification of the mean vascular density (DMV) in samples of normal oral mucosa (MON), oral epithelial dysplasia (ODE) and oral squamous cell carcinoma (COCE). The design - a descriptive study of case series. We analyzed 17 samples of MON, 15 samples of DEO and 16 samples of COCE, stained with TM. To determine usefulness, we compared and analyzed the same samples, either stained with TM or with immunohistochemical technique against CD31. Quantification of the DMV was performed in the 3 areas of greatest vascularization in each sample. DMV was determined according to diagnosis by TM staining and immunohistochemistry against CD31, and the correlation between the two was then calculated. DMV quantified with TM and against CD31 differs according to the diagnosis, with an increase in DMV upon malignant diagnosis. No differences were found when comparing DMV quantified with TM and against CD31. The correlation of the DMV analyzed by TM and against CD31 is significant and moderate. Quantification of blood vessels is possible by TM staining in samples of MON, DEO and COCE. TM staining is moderately correlated with immunohistochemistry against CD31.

KW - Epithelial cells

KW - Mouth neoplasms

KW - Pathological neovascularization

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