Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: The Iberian wolf and leptospires

Javier Millán, Emilio J. García, Álvaro Oleaga, José Vicente López-Bao, Luis Llaneza, Vicente Palacios, Mónica G. Candela, Aitor Cevidanes, Alejandro Rodríguez, Luis León-Vizcaíno

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

The Iberian wolf (Canis lupus) is the top predator in the Iberian environments in which it lives, feeding on a wide range of species, thus encountering a wide range of disease agents. Therefore, the wolf can serve as sentinel of environmental contamination with pathogens. We investigated the exposure of free-living wolves to 14 serovars of Leptospira interrogans sensu lato. Kidney samples from 49 wolves collected from 2010-2013 in northwestern Spain were analysed by culture, direct immunofluorescence and polymerase chain reaction. Tissue fluids were analysed for antibodies by a microscopic agglutination test. Ten wolves (observed prevalence: 20%, 95% confidence interval = 11-33%) showed evidence of contact with leptospires, eight through direct detection and nine through serology (7 wolves were positive according to both techniques). Titres below the cut-off level were also detected in seven cases. Serovars confirmed were Canicola (n = 4), Icterohaemorrhagiae (n = 3) and Sejroë, Ballum and Grippotyphosa (n = 1 each), indicating that wolves were infected with serovars for which dogs, rodents and ungulates, are the natural hosts and supporting the utility of the wolf and other large predators as environmental sentinels for pathogens.

Idioma originalEnglish
Páginas (desde-hasta)1041-1044
Número de páginas4
PublicaciónMemorias do Instituto Oswaldo Cruz
Volumen109
N.º8
DOI
EstadoPublished - 1 dic 2014

Huella dactilar

Bacteria
Leptospira interrogans
cyhalothrin
Direct Fluorescent Antibody Technique
Agglutination Tests
Serology
Spain
Rodentia
Dogs
Confidence Intervals
Kidney
Polymerase Chain Reaction
Antibodies
Serogroup

ASJC Scopus subject areas

  • Microbiology (medical)

Citar esto

Millán, J., García, E. J., Oleaga, Á., López-Bao, J. V., Llaneza, L., Palacios, V., ... León-Vizcaíno, L. (2014). Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: The Iberian wolf and leptospires. Memorias do Instituto Oswaldo Cruz, 109(8), 1041-1044. https://doi.org/10.1590/0074-0276140258
Millán, Javier ; García, Emilio J. ; Oleaga, Álvaro ; López-Bao, José Vicente ; Llaneza, Luis ; Palacios, Vicente ; Candela, Mónica G. ; Cevidanes, Aitor ; Rodríguez, Alejandro ; León-Vizcaíno, Luis. / Using a top predator as a sentinel for environmental contamination with pathogenic bacteria : The Iberian wolf and leptospires. En: Memorias do Instituto Oswaldo Cruz. 2014 ; Vol. 109, N.º 8. pp. 1041-1044.
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abstract = "The Iberian wolf (Canis lupus) is the top predator in the Iberian environments in which it lives, feeding on a wide range of species, thus encountering a wide range of disease agents. Therefore, the wolf can serve as sentinel of environmental contamination with pathogens. We investigated the exposure of free-living wolves to 14 serovars of Leptospira interrogans sensu lato. Kidney samples from 49 wolves collected from 2010-2013 in northwestern Spain were analysed by culture, direct immunofluorescence and polymerase chain reaction. Tissue fluids were analysed for antibodies by a microscopic agglutination test. Ten wolves (observed prevalence: 20{\%}, 95{\%} confidence interval = 11-33{\%}) showed evidence of contact with leptospires, eight through direct detection and nine through serology (7 wolves were positive according to both techniques). Titres below the cut-off level were also detected in seven cases. Serovars confirmed were Canicola (n = 4), Icterohaemorrhagiae (n = 3) and Sejro{\"e}, Ballum and Grippotyphosa (n = 1 each), indicating that wolves were infected with serovars for which dogs, rodents and ungulates, are the natural hosts and supporting the utility of the wolf and other large predators as environmental sentinels for pathogens.",
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Millán, J, García, EJ, Oleaga, Á, López-Bao, JV, Llaneza, L, Palacios, V, Candela, MG, Cevidanes, A, Rodríguez, A & León-Vizcaíno, L 2014, 'Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: The Iberian wolf and leptospires', Memorias do Instituto Oswaldo Cruz, vol. 109, n.º 8, pp. 1041-1044. https://doi.org/10.1590/0074-0276140258

Using a top predator as a sentinel for environmental contamination with pathogenic bacteria : The Iberian wolf and leptospires. / Millán, Javier; García, Emilio J.; Oleaga, Álvaro; López-Bao, José Vicente; Llaneza, Luis; Palacios, Vicente; Candela, Mónica G.; Cevidanes, Aitor; Rodríguez, Alejandro; León-Vizcaíno, Luis.

En: Memorias do Instituto Oswaldo Cruz, Vol. 109, N.º 8, 01.12.2014, p. 1041-1044.

Resultado de la investigación: Article

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T1 - Using a top predator as a sentinel for environmental contamination with pathogenic bacteria

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AU - Millán, Javier

AU - García, Emilio J.

AU - Oleaga, Álvaro

AU - López-Bao, José Vicente

AU - Llaneza, Luis

AU - Palacios, Vicente

AU - Candela, Mónica G.

AU - Cevidanes, Aitor

AU - Rodríguez, Alejandro

AU - León-Vizcaíno, Luis

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