Use of microcosms to determine the survival of the fish pathogen Tenacibaculum maritimum in seawater

Ruben Avendaño-Herrera, Rute Irgang, Beatriz Magariños, Jesús L. Romalde, Alicia E. Toranzo

Resultado de la investigación: Article

15 Citas (Scopus)

Resumen

The survival of the fish pathogen Tenacibaculum maritimum in different seawater microcosms was investigated during 160 days. The persistence of culturable cells was greater in sterile than in natural seawater. Standard plate counts showed that T. maritimum survived in sterile seawater for more than 5 months at concentration around 103 cfu ml-1. However, T. maritimum proved to be very labile in non-sterile seawater, rendering culturable cells no longer than 5 days. These results were confirmed when DNA-based methods were applied. Regardless of the microcosms used, epifluorescence microscopy counts remained at about 106 cells ml-1 throughout the experiment, even though we can not distinguish T. maritimum in the case of non-sterile microcosms. Resuscitation assays with addition of fresh medium to non-sterile microcosms did not favour the recovery of T. maritimum on solid media. Although morphological changes from filamentous to spheres were observed after 3 days in the non-sterile microcosms, in the case of the sterile microcosms this change was observed at the sixth day. The biochemical, physiological, serological and genetic characteristics were unaffected in the sterile microcosms. The overall results contribute to a better understanding of the behaviour of T. maritimum in natural seawater and suggest that the aquatic bacterial population play an important role in the survival of this fish pathogen.

Idioma originalEnglish
Páginas (desde-hasta)921-928
Número de páginas8
PublicaciónEnvironmental Microbiology
Volumen8
N.º5
DOI
EstadoPublished - 1 may 2006

Huella dactilar

Tenacibaculum
Tenacibaculum maritimum
Seawater
microcosm
Fishes
pathogen
seawater
pathogens
fish
Resuscitation
rendering
cells
Microscopy
plate count
microscopy
DNA
persistence
assay

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Citar esto

Avendaño-Herrera, Ruben ; Irgang, Rute ; Magariños, Beatriz ; Romalde, Jesús L. ; Toranzo, Alicia E. / Use of microcosms to determine the survival of the fish pathogen Tenacibaculum maritimum in seawater. En: Environmental Microbiology. 2006 ; Vol. 8, N.º 5. pp. 921-928.
@article{7e3235006e444b52bbdae88be313b8fe,
title = "Use of microcosms to determine the survival of the fish pathogen Tenacibaculum maritimum in seawater",
abstract = "The survival of the fish pathogen Tenacibaculum maritimum in different seawater microcosms was investigated during 160 days. The persistence of culturable cells was greater in sterile than in natural seawater. Standard plate counts showed that T. maritimum survived in sterile seawater for more than 5 months at concentration around 103 cfu ml-1. However, T. maritimum proved to be very labile in non-sterile seawater, rendering culturable cells no longer than 5 days. These results were confirmed when DNA-based methods were applied. Regardless of the microcosms used, epifluorescence microscopy counts remained at about 106 cells ml-1 throughout the experiment, even though we can not distinguish T. maritimum in the case of non-sterile microcosms. Resuscitation assays with addition of fresh medium to non-sterile microcosms did not favour the recovery of T. maritimum on solid media. Although morphological changes from filamentous to spheres were observed after 3 days in the non-sterile microcosms, in the case of the sterile microcosms this change was observed at the sixth day. The biochemical, physiological, serological and genetic characteristics were unaffected in the sterile microcosms. The overall results contribute to a better understanding of the behaviour of T. maritimum in natural seawater and suggest that the aquatic bacterial population play an important role in the survival of this fish pathogen.",
author = "Ruben Avenda{\~n}o-Herrera and Rute Irgang and Beatriz Magari{\~n}os and Romalde, {Jes{\'u}s L.} and Toranzo, {Alicia E.}",
year = "2006",
month = "5",
day = "1",
doi = "10.1111/j.1462-2920.2005.00981.x",
language = "English",
volume = "8",
pages = "921--928",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "Wiley-Blackwell",
number = "5",

}

Use of microcosms to determine the survival of the fish pathogen Tenacibaculum maritimum in seawater. / Avendaño-Herrera, Ruben; Irgang, Rute; Magariños, Beatriz; Romalde, Jesús L.; Toranzo, Alicia E.

En: Environmental Microbiology, Vol. 8, N.º 5, 01.05.2006, p. 921-928.

Resultado de la investigación: Article

TY - JOUR

T1 - Use of microcosms to determine the survival of the fish pathogen Tenacibaculum maritimum in seawater

AU - Avendaño-Herrera, Ruben

AU - Irgang, Rute

AU - Magariños, Beatriz

AU - Romalde, Jesús L.

AU - Toranzo, Alicia E.

PY - 2006/5/1

Y1 - 2006/5/1

N2 - The survival of the fish pathogen Tenacibaculum maritimum in different seawater microcosms was investigated during 160 days. The persistence of culturable cells was greater in sterile than in natural seawater. Standard plate counts showed that T. maritimum survived in sterile seawater for more than 5 months at concentration around 103 cfu ml-1. However, T. maritimum proved to be very labile in non-sterile seawater, rendering culturable cells no longer than 5 days. These results were confirmed when DNA-based methods were applied. Regardless of the microcosms used, epifluorescence microscopy counts remained at about 106 cells ml-1 throughout the experiment, even though we can not distinguish T. maritimum in the case of non-sterile microcosms. Resuscitation assays with addition of fresh medium to non-sterile microcosms did not favour the recovery of T. maritimum on solid media. Although morphological changes from filamentous to spheres were observed after 3 days in the non-sterile microcosms, in the case of the sterile microcosms this change was observed at the sixth day. The biochemical, physiological, serological and genetic characteristics were unaffected in the sterile microcosms. The overall results contribute to a better understanding of the behaviour of T. maritimum in natural seawater and suggest that the aquatic bacterial population play an important role in the survival of this fish pathogen.

AB - The survival of the fish pathogen Tenacibaculum maritimum in different seawater microcosms was investigated during 160 days. The persistence of culturable cells was greater in sterile than in natural seawater. Standard plate counts showed that T. maritimum survived in sterile seawater for more than 5 months at concentration around 103 cfu ml-1. However, T. maritimum proved to be very labile in non-sterile seawater, rendering culturable cells no longer than 5 days. These results were confirmed when DNA-based methods were applied. Regardless of the microcosms used, epifluorescence microscopy counts remained at about 106 cells ml-1 throughout the experiment, even though we can not distinguish T. maritimum in the case of non-sterile microcosms. Resuscitation assays with addition of fresh medium to non-sterile microcosms did not favour the recovery of T. maritimum on solid media. Although morphological changes from filamentous to spheres were observed after 3 days in the non-sterile microcosms, in the case of the sterile microcosms this change was observed at the sixth day. The biochemical, physiological, serological and genetic characteristics were unaffected in the sterile microcosms. The overall results contribute to a better understanding of the behaviour of T. maritimum in natural seawater and suggest that the aquatic bacterial population play an important role in the survival of this fish pathogen.

UR - http://www.scopus.com/inward/record.url?scp=33645461186&partnerID=8YFLogxK

U2 - 10.1111/j.1462-2920.2005.00981.x

DO - 10.1111/j.1462-2920.2005.00981.x

M3 - Article

C2 - 16623748

AN - SCOPUS:33645461186

VL - 8

SP - 921

EP - 928

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 5

ER -