Use of Human Monocyte-Derived Macrophages to Study Neisseria gonorrhoeae Infection

Jimena Gatica, Paula I. Rodas, Alejandro Escobar

Resultado de la investigación: Contribución a los tipos de informe/libroCapítulo

Resumen

Macrophages are critical cells in the innate immune response to microorganisms sensed in the tissues. During infections, the interaction between pathogens and macrophages leads to a macrophage response that includes cytokine production, antigen processing and presentation in the context of MHC molecules, expression of T cell costimulatory molecules and recruitment of innate defense effectors, which results in clearance of infection. However, Neisseria gonorrhoeae can suppress the protective immune response at this level, avoiding its detection and elimination. Studies addressed to develop the interactions between macrophages and Neisseria gonorrhoeae allow us to find potential targets to be exploited with vaccines and therapeutic drugs. In this chapter, we describe protocols to generate human monocyte-derived macrophages and assess their response to infection with Neisseria gonorrhoeae.

Idioma originalInglés
Título de la publicación alojadaMethods in Molecular Biology
EditorialHumana Press Inc.
Páginas301-318
Número de páginas18
DOI
EstadoPublicada - 1 ene 2019

Serie de la publicación

NombreMethods in Molecular Biology
Volumen1997
ISSN (versión impresa)1064-3745

    Huella digital

Áreas temáticas de ASJC Scopus

  • Biología molecular
  • Genética

Citar esto

Gatica, J., Rodas, P. I., & Escobar, A. (2019). Use of Human Monocyte-Derived Macrophages to Study Neisseria gonorrhoeae Infection. En Methods in Molecular Biology (pp. 301-318). (Methods in Molecular Biology; Vol. 1997). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9496-0_18