Uncovering population structure in the Humboldt penguin (Spheniscus humboldti) along the Pacific coast at South America

Gisele P.M. Dantas, Larissa R. Oliveira, Amanda M. Santos, Mariana D. Flores, Daniella R. De Melo, Alejandro Simeone, Daniel González-Acuña, Guillermo Luna-Jorquera, Céline Le Bohec, Armando Valdés-Velásquez, Marco Cardeña, João S. Morgante, Juliana A. Vianna

Resultado de la investigación: Article

Resumen

The upwelling hypothesis has been proposed to explain reduced or lack of population structure in seabird species specialized in food resources available at cold-water upwellings. However, population genetic structure may be challenging to detect in species with large population sizes, since variation in allele frequencies are more robust under genetic drift. High gene flow among populations, that can be constant or pulses of migration in a short period, may also decrease power of algorithms to detect genetic structure. Penguin species usually have large population sizes, high migratory ability but philopatric behavior, and recent investigations debate the existence of subtle population structure for some species not detected before. Previous study on Humboldt penguins found lack of population genetic structure for colonies of Punta San Juan and from South Chile. Here, we used mtDNA and nuclear markers (10 microsatellites and RAG1 intron) to evaluate population structure for 11 main breeding colonies of Humboldt penguins, covering the whole spatial distribution of this species. Although mtDNA failed to detect population structure, microsatellite loci and nuclear intron detected population structure along its latitudinal distribution. Microsatellite showed significant R st values between most of pairwise locations (44 of 56 locations, R st = 0.003 to 0.081) and 86% of individuals were assigned to their sampled colony, suggesting philopatry. STRUCTURE detected three main genetic clusters according to geographical locations: i) Peru; ii) North of Chile; and iii) Central-South of Chile. The Humboldt penguin shows signal population expansion after the Last Glacial Maximum (LGM), suggesting that the genetic structure of the species is a result of population dynamics and foraging colder water upwelling that favor gene flow and phylopatric rate. Our findings thus highlight that variable markers and wide sampling along the species distribution are crucial to better understand genetic population structure in animals with high dispersal ability.

Idioma originalEnglish
Número de artículoe0215293
PublicaciónPLoS ONE
Volumen14
N.º5
DOI
EstadoPublished - 1 may 2019

Huella dactilar

Spheniscidae
Spheniscus
South America
penguins
Microsatellite Repeats
Coastal zones
population structure
Genetic Structures
Mitochondrial DNA
coasts
Introns
Genes
Chile
Population
Population dynamics
Water
microsatellite repeats
Aptitude
Gene Flow
Population Genetics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Citar esto

Dantas, G. P. M., Oliveira, L. R., Santos, A. M., Flores, M. D., De Melo, D. R., Simeone, A., ... Vianna, J. A. (2019). Uncovering population structure in the Humboldt penguin (Spheniscus humboldti) along the Pacific coast at South America. PLoS ONE, 14(5), [e0215293]. https://doi.org/10.1371/journal.pone.0215293
Dantas, Gisele P.M. ; Oliveira, Larissa R. ; Santos, Amanda M. ; Flores, Mariana D. ; De Melo, Daniella R. ; Simeone, Alejandro ; González-Acuña, Daniel ; Luna-Jorquera, Guillermo ; Le Bohec, Céline ; Valdés-Velásquez, Armando ; Cardeña, Marco ; Morgante, João S. ; Vianna, Juliana A. / Uncovering population structure in the Humboldt penguin (Spheniscus humboldti) along the Pacific coast at South America. En: PLoS ONE. 2019 ; Vol. 14, N.º 5.
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abstract = "The upwelling hypothesis has been proposed to explain reduced or lack of population structure in seabird species specialized in food resources available at cold-water upwellings. However, population genetic structure may be challenging to detect in species with large population sizes, since variation in allele frequencies are more robust under genetic drift. High gene flow among populations, that can be constant or pulses of migration in a short period, may also decrease power of algorithms to detect genetic structure. Penguin species usually have large population sizes, high migratory ability but philopatric behavior, and recent investigations debate the existence of subtle population structure for some species not detected before. Previous study on Humboldt penguins found lack of population genetic structure for colonies of Punta San Juan and from South Chile. Here, we used mtDNA and nuclear markers (10 microsatellites and RAG1 intron) to evaluate population structure for 11 main breeding colonies of Humboldt penguins, covering the whole spatial distribution of this species. Although mtDNA failed to detect population structure, microsatellite loci and nuclear intron detected population structure along its latitudinal distribution. Microsatellite showed significant R st values between most of pairwise locations (44 of 56 locations, R st = 0.003 to 0.081) and 86{\%} of individuals were assigned to their sampled colony, suggesting philopatry. STRUCTURE detected three main genetic clusters according to geographical locations: i) Peru; ii) North of Chile; and iii) Central-South of Chile. The Humboldt penguin shows signal population expansion after the Last Glacial Maximum (LGM), suggesting that the genetic structure of the species is a result of population dynamics and foraging colder water upwelling that favor gene flow and phylopatric rate. Our findings thus highlight that variable markers and wide sampling along the species distribution are crucial to better understand genetic population structure in animals with high dispersal ability.",
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Dantas, GPM, Oliveira, LR, Santos, AM, Flores, MD, De Melo, DR, Simeone, A, González-Acuña, D, Luna-Jorquera, G, Le Bohec, C, Valdés-Velásquez, A, Cardeña, M, Morgante, JS & Vianna, JA 2019, 'Uncovering population structure in the Humboldt penguin (Spheniscus humboldti) along the Pacific coast at South America', PLoS ONE, vol. 14, n.º 5, e0215293. https://doi.org/10.1371/journal.pone.0215293

Uncovering population structure in the Humboldt penguin (Spheniscus humboldti) along the Pacific coast at South America. / Dantas, Gisele P.M.; Oliveira, Larissa R.; Santos, Amanda M.; Flores, Mariana D.; De Melo, Daniella R.; Simeone, Alejandro; González-Acuña, Daniel; Luna-Jorquera, Guillermo; Le Bohec, Céline; Valdés-Velásquez, Armando; Cardeña, Marco; Morgante, João S.; Vianna, Juliana A.

En: PLoS ONE, Vol. 14, N.º 5, e0215293, 01.05.2019.

Resultado de la investigación: Article

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AU - Dantas, Gisele P.M.

AU - Oliveira, Larissa R.

AU - Santos, Amanda M.

AU - Flores, Mariana D.

AU - De Melo, Daniella R.

AU - Simeone, Alejandro

AU - González-Acuña, Daniel

AU - Luna-Jorquera, Guillermo

AU - Le Bohec, Céline

AU - Valdés-Velásquez, Armando

AU - Cardeña, Marco

AU - Morgante, João S.

AU - Vianna, Juliana A.

PY - 2019/5/1

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