Two families of repeated DNA sequences in Thiobacillus ferrooxidans

J. R. Yates, D. S. Holmes

Resultado de la investigación: Contribución a una revistaArtículo

57 Citas (Scopus)

Resumen

The genome of Thiobacillus ferrooxidans ATCC 19859 is about 2.8 x 106 base pairs as determined by analysis of reassociation kinetics of sheared DNA. This is 70% of the size of the genome of Escherichia coli. About 6% of the genome of T. ferrooxidans consists of moderately repetitive DNA sequences that are repeated an average of 20 times per genome. Two distinct repeated sequences, designated family 1 and family 2, have been analyzed in more detail. Both families are approximately 1 kilobase in length and are repeated 20 to 30 times per genome. Preliminary evidence from restriction enzyme analysis, Southern blotting experiments, and thermal melting analysis indicates that members of both families are conserved and are interspersed with single-copy DNA. Six copies of one family are present on the 45-kilobase-pair plasmid of strain ATCC 19859.

Idioma originalInglés
Páginas (desde-hasta)1861-1870
Número de páginas10
PublicaciónJournal of Bacteriology
Volumen169
N.º5
EstadoPublicada - 1987

Áreas temáticas de ASJC Scopus

  • Microbiología y biotecnología aplicadas
  • Inmunología
  • Microbiología
  • Biología molecular

Huella Profundice en los temas de investigación de 'Two families of repeated DNA sequences in Thiobacillus ferrooxidans'. En conjunto forman una huella única.

  • Citar esto

    Yates, J. R., & Holmes, D. S. (1987). Two families of repeated DNA sequences in Thiobacillus ferrooxidans. Journal of Bacteriology, 169(5), 1861-1870.