Two families of repeated DNA sequences in Thiobacillus ferrooxidans

J. R. Yates, D. S. Holmes

Resultado de la investigación: Article

55 Citas (Scopus)

Resumen

The genome of Thiobacillus ferrooxidans ATCC 19859 is about 2.8 x 106 base pairs as determined by analysis of reassociation kinetics of sheared DNA. This is 70% of the size of the genome of Escherichia coli. About 6% of the genome of T. ferrooxidans consists of moderately repetitive DNA sequences that are repeated an average of 20 times per genome. Two distinct repeated sequences, designated family 1 and family 2, have been analyzed in more detail. Both families are approximately 1 kilobase in length and are repeated 20 to 30 times per genome. Preliminary evidence from restriction enzyme analysis, Southern blotting experiments, and thermal melting analysis indicates that members of both families are conserved and are interspersed with single-copy DNA. Six copies of one family are present on the 45-kilobase-pair plasmid of strain ATCC 19859.

Idioma originalEnglish
Páginas (desde-hasta)1861-1870
Número de páginas10
PublicaciónJournal of Bacteriology
Volumen169
N.º5
EstadoPublished - 1987

Huella dactilar

Thiobacillus
Genome
Genome Size
Restriction Mapping
Nucleic Acid Repetitive Sequences
DNA
Southern Blotting
Base Pairing
Freezing
Plasmids
Hot Temperature
Escherichia coli

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology
  • Molecular Biology

Citar esto

Yates, J. R., & Holmes, D. S. (1987). Two families of repeated DNA sequences in Thiobacillus ferrooxidans. Journal of Bacteriology, 169(5), 1861-1870.
Yates, J. R. ; Holmes, D. S. / Two families of repeated DNA sequences in Thiobacillus ferrooxidans. En: Journal of Bacteriology. 1987 ; Vol. 169, N.º 5. pp. 1861-1870.
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Yates, JR & Holmes, DS 1987, 'Two families of repeated DNA sequences in Thiobacillus ferrooxidans', Journal of Bacteriology, vol. 169, n.º 5, pp. 1861-1870.

Two families of repeated DNA sequences in Thiobacillus ferrooxidans. / Yates, J. R.; Holmes, D. S.

En: Journal of Bacteriology, Vol. 169, N.º 5, 1987, p. 1861-1870.

Resultado de la investigación: Article

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