Two-dimensional gel electrophoresis analysis of brown algal protein extracts

Loretto Contreras, Andrés Ritter, Geraldine Dennett, Freddy Boehmwald, Nathalie Guitton, Charles Pineau, Alejandra Moenne, Philippe Potin, Juan A. Correa

Resultado de la investigación: Contribución a una revistaArtículo

33 Citas (Scopus)

Resumen

High-quality protein extracts are required for proteomic studies, a field that is poorly developed for marine macroalgae. A reliable phenol extraction protocol using Scytosiphon gracilis Kogame and Ectocarpus siliculosus (Dillwyn) Lyngb. (Phaeophyceae) as algal models resulted in high-quality protein extracts. The performance of the new protocol was tested against four methods available for vascular plants and a seaweed. The protocol, which includes an initial step to remove salts from the algal tissues, allowed the use of highly resolving two-dimensional gel electrophoresis (2-DE) protein analyses, providing the opportunity to unravel potentially novel physiological processes unique to this group of marine organisms.

Idioma originalInglés
Páginas (desde-hasta)1315-1321
Número de páginas7
PublicaciónJournal of Phycology
Volumen44
N.º5
DOI
EstadoPublicada - oct 2008

Áreas temáticas de ASJC Scopus

  • Ciencias acuáticas
  • Botánica

Huella Profundice en los temas de investigación de 'Two-dimensional gel electrophoresis analysis of brown algal protein extracts'. En conjunto forman una huella única.

  • Citar esto

    Contreras, L., Ritter, A., Dennett, G., Boehmwald, F., Guitton, N., Pineau, C., Moenne, A., Potin, P., & Correa, J. A. (2008). Two-dimensional gel electrophoresis analysis of brown algal protein extracts. Journal of Phycology, 44(5), 1315-1321. https://doi.org/10.1111/j.1529-8817.2008.00575.x