@article{508ee9f6e3a74e389af610cdf615fa8f,
title = "TRPV1-Estradiol Stereospecific Relationship Underlies Cell Survival in Oxidative Cell Death",
abstract = "17β-estradiol is a neuronal survival factor against oxidative stress that triggers its protective effect even in the absence of classical estrogen receptors. The polymodal transient receptor potential vanilloid subtype 1 (TRPV1) channel has been proposed as a steroid receptor implied in tissue protection against oxidative damage. We show here that TRPV1 is sufficient condition for 17β-estradiol to enhance metabolic performance in injured cells. Specifically, in TRPV1 expressing cells, the application of 17β-estradiol within the first 3 h avoided H2O2-dependent mitochondrial depolarization and the activation of caspase 3/7 protecting against the irreversible damage triggered by H2O2. Furthermore, 17β-estradiol potentiates TRPV1 single channel activity associated with an increased open probability. This effect was not observed after the application of 17α-estradiol. We explored the TRPV1-Estrogen relationship also in primary culture of hippocampal-derived neurons and observed that 17β-estradiol cell protection against H2O2-induced damage was independent of estrogen receptors pathway activation, membrane started and stereospecific. These results support the role of TRPV1 as a 17β-estradiol-activated ionotropic membrane receptor coupling with mitochondrial function and cell survival.",
keywords = "17β-estradiol, cell death, membrane receptor, neuroprotection, TRPV1",
author = "Ricardo Ram{\'i}rez-Barrantes and Karina Carvajal-Zamorano and Belen Rodriguez and Claudio Cordova and Carlo Lozano and Felipe Simon and Paula D{\'i}az and Pablo Mu{\~n}oz and Ivanny Marchant and Ram{\'o}n Latorre and Karen Castillo and Pablo Olivero",
note = "Funding Information: We thank the Doctoral Program in Neuroscience of Universidad de Valparaiso and CONICYT-Chile (KC-Z) for a doctoral fellowship. We thank Luisa Soto for excellent technical assistance. Funding. This collaborative work was supported by research grants from Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico: Fondecyt 11100047, DIUV 21 and Anillo ACT-1104 (PO), Fondecyt 1121078 (FS), FONDECYT 1180999 (KC), FONDECYT 1190203 (RL). The Millennium Nucleus of Ion Channels-Associated Diseases (MiNICAD) is a Millennium Nucleus supported by the Iniciativa Cient?fica Milenio of the Ministry of Economy, Development and Tourism (Chile) (FS). The Centro Interdisciplinario de Neurociencia de Valparaiso is a Millennium Institute supported by the Millennium Scientific Initiative of the Chilean Ministry of Economy, Development, and Tourism (P029-022-F). Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2020 Ram{\'i}rez-Barrantes, Carvajal-Zamorano, Rodriguez, Cordova, Lozano, Simon, D{\'i}az, Mu{\~n}oz, Marchant, Latorre, Castillo and Olivero.",
year = "2020",
month = may,
day = "26",
doi = "10.3389/fphys.2020.00444",
language = "English",
volume = "11",
journal = "Frontiers in Physiology",
issn = "1664-042X",
publisher = "Frontiers Media S.A.",
}