Transcriptomics using next generation sequencing technologies

Dasfne Lee-Liu, Leonardo I. Almonacid, Fernando Faunes, Francisco Melo, Juan Larrain

Resultado de la investigación: Chapter

6 Citas (Scopus)

Resumen

Next generation sequencing technologies may now be applied to the study of transcriptomics. RNA-Seq or RNA sequencing employs high-throughput sequencing of complementary DNA fragments delivering a transcriptional profile. In this chapter, we aim to provide a starting point for Xenopus researchers planning on starting an RNA-Seq transcriptomics study. We begin by providing a section on template isolation and library preparation. The next section comprises the main bioinformatics procedures that need to be performed for raw data processing, normalization, and differential gene expression. Finally, we have included a section on studying deep sequencing results in Xenopus, which offers general guidance as to what can be done in this model.

Idioma originalEnglish
Título de la publicación alojadaXenopus Protocols
Páginas293-317
Número de páginas25
Volumen917
DOI
EstadoPublished - 2012

Serie de la publicación

NombreMethods in Molecular Biology
Volumen917
ISSN (versión impresa)10643745

Huella dactilar

High-Throughput Nucleotide Sequencing
Xenopus
RNA
Technology
Computational Biology
Libraries
Complementary DNA
Research Personnel
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Citar esto

Lee-Liu, D., Almonacid, L. I., Faunes, F., Melo, F., & Larrain, J. (2012). Transcriptomics using next generation sequencing technologies. En Xenopus Protocols (Vol. 917, pp. 293-317). (Methods in Molecular Biology; Vol. 917). https://doi.org/10.1007/978-1-61779-992-1-18
Lee-Liu, Dasfne ; Almonacid, Leonardo I. ; Faunes, Fernando ; Melo, Francisco ; Larrain, Juan. / Transcriptomics using next generation sequencing technologies. Xenopus Protocols. Vol. 917 2012. pp. 293-317 (Methods in Molecular Biology).
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Lee-Liu, D, Almonacid, LI, Faunes, F, Melo, F & Larrain, J 2012, Transcriptomics using next generation sequencing technologies. En Xenopus Protocols. vol. 917, Methods in Molecular Biology, vol. 917, pp. 293-317. https://doi.org/10.1007/978-1-61779-992-1-18

Transcriptomics using next generation sequencing technologies. / Lee-Liu, Dasfne; Almonacid, Leonardo I.; Faunes, Fernando; Melo, Francisco; Larrain, Juan.

Xenopus Protocols. Vol. 917 2012. p. 293-317 (Methods in Molecular Biology; Vol. 917).

Resultado de la investigación: Chapter

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Lee-Liu D, Almonacid LI, Faunes F, Melo F, Larrain J. Transcriptomics using next generation sequencing technologies. En Xenopus Protocols. Vol. 917. 2012. p. 293-317. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-992-1-18