Transcriptomic response of rainbow trout (Oncorhynchus mykiss)skeletal muscle to Flavobacterium psychrophilum

Andrea Rivas-Aravena, Marcia Fuentes-Valenzuela, Sebastian Escobar-Aguirre, Cristian Gallardo-Escarate, Alfredo Molina, Juan Antonio Valdés

Resultado de la investigación: Article

Resumen

Flavobacterium psychrophilum is the etiologic agent of rainbow trout fry syndrome (RTFS). This pathogen infects a wide variety of salmonid species during freshwater stages, causing significant losses in the aquaculture industry. Rainbow trout (Oncorhynchus mykiss)infected with F. psychrophilum, presents as the main external clinical sign ulcerative lesions and necrotic myositis in skeletal muscle. We previously reported the in vitro cytotoxic activity of F. psychrophilum on rainbow trout myoblast, however little is known about the molecular mechanisms underlying the in vivo pathogenesis in skeletal muscle. In this study, we examined the transcriptomic profiles of skeletal muscle tissue of rainbow trout intraperitoneally challenged with low infection dose of F. psychrophilum. Using high-throughput RNA-seq, we found that 233 transcripts were up-regulated, mostly associated to ubiquitin mediated proteolysis and apoptosis. Conversely, 189 transcripts were down-regulated, associated to skeletal muscle contraction. This molecular signature was consistent with creatine kinase activity in plasma and oxidative damage in skeletal muscle. Moreover, the increased caspase activity suggests as a whole skeletal muscle atrophy induced by F. psychrophilum. This study offers an integrative analysis of the skeletal muscle response to F. psychrophilum infection and reveals unknown aspects of its pathogenesis in rainbow trout.

Idioma originalEnglish
Número de artículo100596
PublicaciónComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volumen31
DOI
EstadoPublished - 1 sep 2019

Huella dactilar

Flavobacterium
Oncorhynchus mykiss
Muscle
Skeletal Muscle
Proteolysis
Aquaculture
Myositis
Muscular Atrophy
Myoblasts
Pathogens
Creatine Kinase
Muscle Contraction
Caspases
Ubiquitin
Infection
Fresh Water
Industry
Throughput
RNA
Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Genetics

Citar esto

Rivas-Aravena, Andrea ; Fuentes-Valenzuela, Marcia ; Escobar-Aguirre, Sebastian ; Gallardo-Escarate, Cristian ; Molina, Alfredo ; Valdés, Juan Antonio. / Transcriptomic response of rainbow trout (Oncorhynchus mykiss)skeletal muscle to Flavobacterium psychrophilum. En: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics. 2019 ; Vol. 31.
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abstract = "Flavobacterium psychrophilum is the etiologic agent of rainbow trout fry syndrome (RTFS). This pathogen infects a wide variety of salmonid species during freshwater stages, causing significant losses in the aquaculture industry. Rainbow trout (Oncorhynchus mykiss)infected with F. psychrophilum, presents as the main external clinical sign ulcerative lesions and necrotic myositis in skeletal muscle. We previously reported the in vitro cytotoxic activity of F. psychrophilum on rainbow trout myoblast, however little is known about the molecular mechanisms underlying the in vivo pathogenesis in skeletal muscle. In this study, we examined the transcriptomic profiles of skeletal muscle tissue of rainbow trout intraperitoneally challenged with low infection dose of F. psychrophilum. Using high-throughput RNA-seq, we found that 233 transcripts were up-regulated, mostly associated to ubiquitin mediated proteolysis and apoptosis. Conversely, 189 transcripts were down-regulated, associated to skeletal muscle contraction. This molecular signature was consistent with creatine kinase activity in plasma and oxidative damage in skeletal muscle. Moreover, the increased caspase activity suggests as a whole skeletal muscle atrophy induced by F. psychrophilum. This study offers an integrative analysis of the skeletal muscle response to F. psychrophilum infection and reveals unknown aspects of its pathogenesis in rainbow trout.",
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Transcriptomic response of rainbow trout (Oncorhynchus mykiss)skeletal muscle to Flavobacterium psychrophilum. / Rivas-Aravena, Andrea; Fuentes-Valenzuela, Marcia; Escobar-Aguirre, Sebastian; Gallardo-Escarate, Cristian; Molina, Alfredo; Valdés, Juan Antonio.

En: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Vol. 31, 100596, 01.09.2019.

Resultado de la investigación: Article

TY - JOUR

T1 - Transcriptomic response of rainbow trout (Oncorhynchus mykiss)skeletal muscle to Flavobacterium psychrophilum

AU - Rivas-Aravena, Andrea

AU - Fuentes-Valenzuela, Marcia

AU - Escobar-Aguirre, Sebastian

AU - Gallardo-Escarate, Cristian

AU - Molina, Alfredo

AU - Valdés, Juan Antonio

PY - 2019/9/1

Y1 - 2019/9/1

N2 - Flavobacterium psychrophilum is the etiologic agent of rainbow trout fry syndrome (RTFS). This pathogen infects a wide variety of salmonid species during freshwater stages, causing significant losses in the aquaculture industry. Rainbow trout (Oncorhynchus mykiss)infected with F. psychrophilum, presents as the main external clinical sign ulcerative lesions and necrotic myositis in skeletal muscle. We previously reported the in vitro cytotoxic activity of F. psychrophilum on rainbow trout myoblast, however little is known about the molecular mechanisms underlying the in vivo pathogenesis in skeletal muscle. In this study, we examined the transcriptomic profiles of skeletal muscle tissue of rainbow trout intraperitoneally challenged with low infection dose of F. psychrophilum. Using high-throughput RNA-seq, we found that 233 transcripts were up-regulated, mostly associated to ubiquitin mediated proteolysis and apoptosis. Conversely, 189 transcripts were down-regulated, associated to skeletal muscle contraction. This molecular signature was consistent with creatine kinase activity in plasma and oxidative damage in skeletal muscle. Moreover, the increased caspase activity suggests as a whole skeletal muscle atrophy induced by F. psychrophilum. This study offers an integrative analysis of the skeletal muscle response to F. psychrophilum infection and reveals unknown aspects of its pathogenesis in rainbow trout.

AB - Flavobacterium psychrophilum is the etiologic agent of rainbow trout fry syndrome (RTFS). This pathogen infects a wide variety of salmonid species during freshwater stages, causing significant losses in the aquaculture industry. Rainbow trout (Oncorhynchus mykiss)infected with F. psychrophilum, presents as the main external clinical sign ulcerative lesions and necrotic myositis in skeletal muscle. We previously reported the in vitro cytotoxic activity of F. psychrophilum on rainbow trout myoblast, however little is known about the molecular mechanisms underlying the in vivo pathogenesis in skeletal muscle. In this study, we examined the transcriptomic profiles of skeletal muscle tissue of rainbow trout intraperitoneally challenged with low infection dose of F. psychrophilum. Using high-throughput RNA-seq, we found that 233 transcripts were up-regulated, mostly associated to ubiquitin mediated proteolysis and apoptosis. Conversely, 189 transcripts were down-regulated, associated to skeletal muscle contraction. This molecular signature was consistent with creatine kinase activity in plasma and oxidative damage in skeletal muscle. Moreover, the increased caspase activity suggests as a whole skeletal muscle atrophy induced by F. psychrophilum. This study offers an integrative analysis of the skeletal muscle response to F. psychrophilum infection and reveals unknown aspects of its pathogenesis in rainbow trout.

KW - Flavobacterium psychrophilum

KW - RNA-seq, rainbow trout

KW - Skeletal muscle atrophy

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