Transcriptome profiling of Botrytis cinerea conidial germination reveals upregulation of infection-related genes during the prepenetration stage

Michaela Leroch, Astrid Kleber, Evelyn Silva, Tina Coenen, Dieter Koppenhöfer, Amir Shmaryahu, Pablo D T Valenzuela, Matthias Hahn

Resultado de la investigación: Article

35 Citas (Scopus)

Resumen

Botrytis cinerea causes gray mold on a great number of host plants. Infection is initiated by airborne conidia that invade the host tissue, often by penetration of intact epidermal cells. To mimic the surface properties of natural plant surfaces, conidia were incubated on apple wax-coated surfaces, resulting in rapid germination and appressorium formation. Global changes in gene expression were analyzed by microarray hybridization between conidia incubated for 0 h (dormant), 1 h (pregermination), 2.5 h (postgermination), 4 h (appressoria), and 15 h (early mycelium). Considerable changes were observed, in particular between 0 h and 1 h. Genes induced during germination were enriched in those genes encoding secreted proteins, including lytic enzymes. Comparison of wild-type and a nonpathogenic MAP kinase mutant (bmp1) revealed marked differences in germination-related gene expression, in particular related to secretory proteins. Using promoter-GFP reporter strains, we detected a strictly germination-specific expression pattern of a putative chitin deacetylase gene (cda1). In contrast, a cutinase gene (cutB) was found to be expressed only in the presence of plant lipids, in a developmentally less stringent pattern. We also identified a coregulated gene cluster possibly involved in secondary metabolite synthesis which was found to be controlled by a transcription factor also encoded in this cluster. Our data demonstrate that early conidial development in B. cinerea is accompanied by rapid shifts in gene expression that prepare the fungus for germ tube outgrowth and host cell invasion.

Idioma originalEnglish
Páginas (desde-hasta)614-626
Número de páginas13
PublicaciónEukaryotic Cell
Volumen12
N.º4
DOI
EstadoPublished - abr 2013

Huella dactilar

Botrytis
Gene Expression Profiling
Germination
Fungal Spores
Up-Regulation
chitin deacetylase
Infection
Gene Expression
Genes
Fungi
Surface Properties
Waxes
Mycelium
Malus
Multigene Family
Proteins
Transcription Factors
Phosphotransferases
Lipids
Enzymes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Medicine(all)

Citar esto

Leroch, Michaela ; Kleber, Astrid ; Silva, Evelyn ; Coenen, Tina ; Koppenhöfer, Dieter ; Shmaryahu, Amir ; Valenzuela, Pablo D T ; Hahn, Matthias. / Transcriptome profiling of Botrytis cinerea conidial germination reveals upregulation of infection-related genes during the prepenetration stage. En: Eukaryotic Cell. 2013 ; Vol. 12, N.º 4. pp. 614-626.
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title = "Transcriptome profiling of Botrytis cinerea conidial germination reveals upregulation of infection-related genes during the prepenetration stage",
abstract = "Botrytis cinerea causes gray mold on a great number of host plants. Infection is initiated by airborne conidia that invade the host tissue, often by penetration of intact epidermal cells. To mimic the surface properties of natural plant surfaces, conidia were incubated on apple wax-coated surfaces, resulting in rapid germination and appressorium formation. Global changes in gene expression were analyzed by microarray hybridization between conidia incubated for 0 h (dormant), 1 h (pregermination), 2.5 h (postgermination), 4 h (appressoria), and 15 h (early mycelium). Considerable changes were observed, in particular between 0 h and 1 h. Genes induced during germination were enriched in those genes encoding secreted proteins, including lytic enzymes. Comparison of wild-type and a nonpathogenic MAP kinase mutant (bmp1) revealed marked differences in germination-related gene expression, in particular related to secretory proteins. Using promoter-GFP reporter strains, we detected a strictly germination-specific expression pattern of a putative chitin deacetylase gene (cda1). In contrast, a cutinase gene (cutB) was found to be expressed only in the presence of plant lipids, in a developmentally less stringent pattern. We also identified a coregulated gene cluster possibly involved in secondary metabolite synthesis which was found to be controlled by a transcription factor also encoded in this cluster. Our data demonstrate that early conidial development in B. cinerea is accompanied by rapid shifts in gene expression that prepare the fungus for germ tube outgrowth and host cell invasion.",
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Leroch, M, Kleber, A, Silva, E, Coenen, T, Koppenhöfer, D, Shmaryahu, A, Valenzuela, PDT & Hahn, M 2013, 'Transcriptome profiling of Botrytis cinerea conidial germination reveals upregulation of infection-related genes during the prepenetration stage', Eukaryotic Cell, vol. 12, n.º 4, pp. 614-626. https://doi.org/10.1128/EC.00295-12

Transcriptome profiling of Botrytis cinerea conidial germination reveals upregulation of infection-related genes during the prepenetration stage. / Leroch, Michaela; Kleber, Astrid; Silva, Evelyn; Coenen, Tina; Koppenhöfer, Dieter; Shmaryahu, Amir; Valenzuela, Pablo D T; Hahn, Matthias.

En: Eukaryotic Cell, Vol. 12, N.º 4, 04.2013, p. 614-626.

Resultado de la investigación: Article

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AU - Leroch, Michaela

AU - Kleber, Astrid

AU - Silva, Evelyn

AU - Coenen, Tina

AU - Koppenhöfer, Dieter

AU - Shmaryahu, Amir

AU - Valenzuela, Pablo D T

AU - Hahn, Matthias

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