Transcriptome analysis reveals regulatory networks underlying differential susceptibility to Botrytis cinerea in response to nitrogen availability in Solanum lycopersicum

Andrea Vega, Paulo Canessa, Gustavo Hoppe, Ignacio Retamal, Tomas C. Moyano, Javier Canales, Rodrigo A. Gutiérrez, Joselyn Rubilar

Resultado de la investigación: Article

9 Citas (Scopus)

Resumen

Nitrogen (N) is one of the main limiting nutrients for plant growth and crop yield. It is well documented that changes in nitrate availability, the main N source found in agricultural soils, influences a myriad of developmental programs and processes including the plant defense response. Indeed, many agronomical reports indicate that the plant N nutritional status influences their ability to respond effectively when challenged by different pathogens. However, the molecular mechanisms involved in N-modulation of plant susceptibility to pathogens are poorly characterized. In this work, we show that Solanum lycopersicum defense response to the necrotrophic fungus Botrytis cinerea is affected by plant N availability, with higher susceptibility in nitrate-limiting conditions. Global gene expression responses of tomato against B. cinerea under contrasting nitrate conditions reveals that plant primary metabolism is affected by the fungal infection regardless of N regimes. This result suggests that differential susceptibility to pathogen attack under contrasting N conditions is not only explained by a metabolic alteration. We used a systems biology approach to identify the transcriptional regulatory network implicated in plant response to the fungus infection under contrasting nitrate conditions. Interestingly, hub genes in this network are known key transcription factors involved in ethylene and jasmonic acid signaling. This result positions these hormones as key integrators of nitrate and defense against B. cinerea in tomato plants. Our results provide insights into potential crosstalk mechanisms between necrotrophic defense response and N status in plants.

Idioma originalEnglish
Número de artículo911
PublicaciónFrontiers in Plant Science
Volumen6
N.ºNOVEMBER
DOI
EstadoPublished - 4 nov 2015

Huella dactilar

Solanum lycopersicum
Botrytis cinerea
transcriptomics
nitrogen
nitrates
pathogens
tomatoes
fungi
jasmonic acid
agricultural soils
infection
nutritional status
plant response
crop yield
ethylene
transcription factors
hormones
plant growth
Biological Sciences
gene expression

ASJC Scopus subject areas

  • Plant Science

Citar esto

Vega, Andrea ; Canessa, Paulo ; Hoppe, Gustavo ; Retamal, Ignacio ; Moyano, Tomas C. ; Canales, Javier ; Gutiérrez, Rodrigo A. ; Rubilar, Joselyn. / Transcriptome analysis reveals regulatory networks underlying differential susceptibility to Botrytis cinerea in response to nitrogen availability in Solanum lycopersicum. En: Frontiers in Plant Science. 2015 ; Vol. 6, N.º NOVEMBER.
@article{8cf27164ae5546d0b0519b5ef8717cf0,
title = "Transcriptome analysis reveals regulatory networks underlying differential susceptibility to Botrytis cinerea in response to nitrogen availability in Solanum lycopersicum",
abstract = "Nitrogen (N) is one of the main limiting nutrients for plant growth and crop yield. It is well documented that changes in nitrate availability, the main N source found in agricultural soils, influences a myriad of developmental programs and processes including the plant defense response. Indeed, many agronomical reports indicate that the plant N nutritional status influences their ability to respond effectively when challenged by different pathogens. However, the molecular mechanisms involved in N-modulation of plant susceptibility to pathogens are poorly characterized. In this work, we show that Solanum lycopersicum defense response to the necrotrophic fungus Botrytis cinerea is affected by plant N availability, with higher susceptibility in nitrate-limiting conditions. Global gene expression responses of tomato against B. cinerea under contrasting nitrate conditions reveals that plant primary metabolism is affected by the fungal infection regardless of N regimes. This result suggests that differential susceptibility to pathogen attack under contrasting N conditions is not only explained by a metabolic alteration. We used a systems biology approach to identify the transcriptional regulatory network implicated in plant response to the fungus infection under contrasting nitrate conditions. Interestingly, hub genes in this network are known key transcription factors involved in ethylene and jasmonic acid signaling. This result positions these hormones as key integrators of nitrate and defense against B. cinerea in tomato plants. Our results provide insights into potential crosstalk mechanisms between necrotrophic defense response and N status in plants.",
keywords = "Defense mechanisms, Ethylene signaling, Gene network analysis, Jasmonic acid, Microarray analysis, Nitrate nutrition, Tomato",
author = "Andrea Vega and Paulo Canessa and Gustavo Hoppe and Ignacio Retamal and Moyano, {Tomas C.} and Javier Canales and Guti{\'e}rrez, {Rodrigo A.} and Joselyn Rubilar",
year = "2015",
month = "11",
day = "4",
doi = "10.3389/fpls.2015.00911",
language = "English",
volume = "6",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media S.A.",
number = "NOVEMBER",

}

Transcriptome analysis reveals regulatory networks underlying differential susceptibility to Botrytis cinerea in response to nitrogen availability in Solanum lycopersicum. / Vega, Andrea; Canessa, Paulo; Hoppe, Gustavo; Retamal, Ignacio; Moyano, Tomas C.; Canales, Javier; Gutiérrez, Rodrigo A.; Rubilar, Joselyn.

En: Frontiers in Plant Science, Vol. 6, N.º NOVEMBER, 911, 04.11.2015.

Resultado de la investigación: Article

TY - JOUR

T1 - Transcriptome analysis reveals regulatory networks underlying differential susceptibility to Botrytis cinerea in response to nitrogen availability in Solanum lycopersicum

AU - Vega, Andrea

AU - Canessa, Paulo

AU - Hoppe, Gustavo

AU - Retamal, Ignacio

AU - Moyano, Tomas C.

AU - Canales, Javier

AU - Gutiérrez, Rodrigo A.

AU - Rubilar, Joselyn

PY - 2015/11/4

Y1 - 2015/11/4

N2 - Nitrogen (N) is one of the main limiting nutrients for plant growth and crop yield. It is well documented that changes in nitrate availability, the main N source found in agricultural soils, influences a myriad of developmental programs and processes including the plant defense response. Indeed, many agronomical reports indicate that the plant N nutritional status influences their ability to respond effectively when challenged by different pathogens. However, the molecular mechanisms involved in N-modulation of plant susceptibility to pathogens are poorly characterized. In this work, we show that Solanum lycopersicum defense response to the necrotrophic fungus Botrytis cinerea is affected by plant N availability, with higher susceptibility in nitrate-limiting conditions. Global gene expression responses of tomato against B. cinerea under contrasting nitrate conditions reveals that plant primary metabolism is affected by the fungal infection regardless of N regimes. This result suggests that differential susceptibility to pathogen attack under contrasting N conditions is not only explained by a metabolic alteration. We used a systems biology approach to identify the transcriptional regulatory network implicated in plant response to the fungus infection under contrasting nitrate conditions. Interestingly, hub genes in this network are known key transcription factors involved in ethylene and jasmonic acid signaling. This result positions these hormones as key integrators of nitrate and defense against B. cinerea in tomato plants. Our results provide insights into potential crosstalk mechanisms between necrotrophic defense response and N status in plants.

AB - Nitrogen (N) is one of the main limiting nutrients for plant growth and crop yield. It is well documented that changes in nitrate availability, the main N source found in agricultural soils, influences a myriad of developmental programs and processes including the plant defense response. Indeed, many agronomical reports indicate that the plant N nutritional status influences their ability to respond effectively when challenged by different pathogens. However, the molecular mechanisms involved in N-modulation of plant susceptibility to pathogens are poorly characterized. In this work, we show that Solanum lycopersicum defense response to the necrotrophic fungus Botrytis cinerea is affected by plant N availability, with higher susceptibility in nitrate-limiting conditions. Global gene expression responses of tomato against B. cinerea under contrasting nitrate conditions reveals that plant primary metabolism is affected by the fungal infection regardless of N regimes. This result suggests that differential susceptibility to pathogen attack under contrasting N conditions is not only explained by a metabolic alteration. We used a systems biology approach to identify the transcriptional regulatory network implicated in plant response to the fungus infection under contrasting nitrate conditions. Interestingly, hub genes in this network are known key transcription factors involved in ethylene and jasmonic acid signaling. This result positions these hormones as key integrators of nitrate and defense against B. cinerea in tomato plants. Our results provide insights into potential crosstalk mechanisms between necrotrophic defense response and N status in plants.

KW - Defense mechanisms

KW - Ethylene signaling

KW - Gene network analysis

KW - Jasmonic acid

KW - Microarray analysis

KW - Nitrate nutrition

KW - Tomato

UR - http://www.scopus.com/inward/record.url?scp=84947441247&partnerID=8YFLogxK

U2 - 10.3389/fpls.2015.00911

DO - 10.3389/fpls.2015.00911

M3 - Article

AN - SCOPUS:84947441247

VL - 6

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

IS - NOVEMBER

M1 - 911

ER -