Transcriptome analysis reveals novel genes involved in nonhost response to bacterial infection in tobacco

Lucas Damián Daurelio, Silvana Petrocelli, Francisca Blanco, Loreto Holuigue, Jorgelina Ottado, Elena Graciela Orellano

Resultado de la investigación: Article

17 Citas (Scopus)

Resumen

Plants are continuously exposed to pathogen challenge. The most common defense response to pathogenic microorganisms is the nonhost response, which is usually accompanied by transcriptional changes. In order to identify genes involved in nonhost resistance, we evaluated the tobacco transcriptome profile after infection with Xanthomonas axonopodis pv. citri (Xac), a nonhost phytopathogenic bacterium. cDNA-amplified fragment length polymorphism was used to identify differentially expressed transcripts in tobacco leaves infected with Xac at 2, 8 and 24. h post-inoculation. From a total of 2087 transcript-derived fragments (TDFs) screened (approximately 20% of the tobacco transcriptome), 316 TDFs showed differential expression. Based on sequence similarities, 82 differential TDFs were identified and assigned to different functional categories: 56 displayed homology to genes with known functions, 12 to proteins with unknown functions and 14 did not have a match. Real-time PCR was carried out with selected transcripts to confirm the expression pattern obtained. The results reveal novel genes associated with nonhost resistance in plant-pathogen interaction in tobacco. These novel genes could be included in future strategies of molecular breeding for nonhost disease resistance.

Idioma originalEnglish
Páginas (desde-hasta)382-391
Número de páginas10
PublicaciónJournal of Plant Physiology
Volumen168
N.º4
DOI
EstadoPublished - 1 mar 2011

Huella dactilar

Gene Expression Profiling
bacterial infections
transcriptomics
Bacterial Infections
Tobacco
tobacco
Xanthomonas axonopodis pv. citri
Transcriptome
transcriptome
Genes
Xanthomonas axonopodis
genes
DNA Shuffling
plant pathogenic bacteria
Disease Resistance
plant pathogens
amplified fragment length polymorphism
disease resistance
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Citar esto

Daurelio, Lucas Damián ; Petrocelli, Silvana ; Blanco, Francisca ; Holuigue, Loreto ; Ottado, Jorgelina ; Orellano, Elena Graciela. / Transcriptome analysis reveals novel genes involved in nonhost response to bacterial infection in tobacco. En: Journal of Plant Physiology. 2011 ; Vol. 168, N.º 4. pp. 382-391.
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abstract = "Plants are continuously exposed to pathogen challenge. The most common defense response to pathogenic microorganisms is the nonhost response, which is usually accompanied by transcriptional changes. In order to identify genes involved in nonhost resistance, we evaluated the tobacco transcriptome profile after infection with Xanthomonas axonopodis pv. citri (Xac), a nonhost phytopathogenic bacterium. cDNA-amplified fragment length polymorphism was used to identify differentially expressed transcripts in tobacco leaves infected with Xac at 2, 8 and 24. h post-inoculation. From a total of 2087 transcript-derived fragments (TDFs) screened (approximately 20{\%} of the tobacco transcriptome), 316 TDFs showed differential expression. Based on sequence similarities, 82 differential TDFs were identified and assigned to different functional categories: 56 displayed homology to genes with known functions, 12 to proteins with unknown functions and 14 did not have a match. Real-time PCR was carried out with selected transcripts to confirm the expression pattern obtained. The results reveal novel genes associated with nonhost resistance in plant-pathogen interaction in tobacco. These novel genes could be included in future strategies of molecular breeding for nonhost disease resistance.",
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Transcriptome analysis reveals novel genes involved in nonhost response to bacterial infection in tobacco. / Daurelio, Lucas Damián; Petrocelli, Silvana; Blanco, Francisca; Holuigue, Loreto; Ottado, Jorgelina; Orellano, Elena Graciela.

En: Journal of Plant Physiology, Vol. 168, N.º 4, 01.03.2011, p. 382-391.

Resultado de la investigación: Article

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