Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis

Ariel Herrera-Vásquez, Loreto Carvallo, Francisca Blanco, Mariola Tobar, Eva Villarroel-Candia, Jesús Vicente-Carbajosa, Paula Salinas, Loreto Holuigue

Resultado de la investigación: Article

19 Citas (Scopus)

Resumen

Salicylic acid (SA) is a key hormone that mediates gene transcriptional reprogramming in the context of the defense response to stress. GRXC9, coding for a CC-type glutaredoxin from Arabidopsis, is an SA-responsive gene induced early and transiently by an NPR1-independent pathway. Here, we address the mechanism involved in this SA-dependent pathway, using GRXC9 as a model gene. We first established that GRXC9 expression is induced by UVB exposure through this pathway, validating its activation in a physiological stress condition. GRXC9 promoter analyses indicate that SA controls gene transcription through two activating sequence-1 (as-1)-like elements located in its proximal region. TGA2 and TGA3, but not TGA1, are constitutively bound to this promoter region. Accordingly, the transient recruitment of RNA polymerase II to the GRXC9 promoter, as well as the transient accumulation of gene transcripts detected in SA-treated WT plants, was abolished in a knockout mutant for the TGA class II factors. We conclude that constitutive binding of TGA2 is essential for controlling GRXC9 expression, while binding of TGA3 in a lesser extent contributes to this regulation. Finally, overexpression of GRXC9 indicates that the GRXC9 protein negatively controls its own gene expression, forming part of the complex bound to the as-1-containing promoter region. These findings are integrated in a model that explains how SA controls transcription of GRXC9 in the context of the defense response to stress.

Idioma originalEnglish
Páginas (desde-hasta)624-637
Número de páginas14
PublicaciónPlant Molecular Biology Reporter
Volumen33
N.º3
DOI
EstadoPublished - 16 jun 2015

Huella dactilar

Glutaredoxins
Salicylic Acid
Arabidopsis
salicylic acid
promoter regions
Genes
genes
Genetic Promoter Regions
stress response
transcription (genetics)
exposure pathways
Physiological Stress
knockout mutants
RNA Polymerase II
DNA-directed RNA polymerase
hormones
Hormones
Gene Expression
gene expression

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science

Citar esto

Herrera-Vásquez, Ariel ; Carvallo, Loreto ; Blanco, Francisca ; Tobar, Mariola ; Villarroel-Candia, Eva ; Vicente-Carbajosa, Jesús ; Salinas, Paula ; Holuigue, Loreto. / Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis. En: Plant Molecular Biology Reporter. 2015 ; Vol. 33, N.º 3. pp. 624-637.
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title = "Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis",
abstract = "Salicylic acid (SA) is a key hormone that mediates gene transcriptional reprogramming in the context of the defense response to stress. GRXC9, coding for a CC-type glutaredoxin from Arabidopsis, is an SA-responsive gene induced early and transiently by an NPR1-independent pathway. Here, we address the mechanism involved in this SA-dependent pathway, using GRXC9 as a model gene. We first established that GRXC9 expression is induced by UVB exposure through this pathway, validating its activation in a physiological stress condition. GRXC9 promoter analyses indicate that SA controls gene transcription through two activating sequence-1 (as-1)-like elements located in its proximal region. TGA2 and TGA3, but not TGA1, are constitutively bound to this promoter region. Accordingly, the transient recruitment of RNA polymerase II to the GRXC9 promoter, as well as the transient accumulation of gene transcripts detected in SA-treated WT plants, was abolished in a knockout mutant for the TGA class II factors. We conclude that constitutive binding of TGA2 is essential for controlling GRXC9 expression, while binding of TGA3 in a lesser extent contributes to this regulation. Finally, overexpression of GRXC9 indicates that the GRXC9 protein negatively controls its own gene expression, forming part of the complex bound to the as-1-containing promoter region. These findings are integrated in a model that explains how SA controls transcription of GRXC9 in the context of the defense response to stress.",
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author = "Ariel Herrera-V{\'a}squez and Loreto Carvallo and Francisca Blanco and Mariola Tobar and Eva Villarroel-Candia and Jes{\'u}s Vicente-Carbajosa and Paula Salinas and Loreto Holuigue",
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Herrera-Vásquez, A, Carvallo, L, Blanco, F, Tobar, M, Villarroel-Candia, E, Vicente-Carbajosa, J, Salinas, P & Holuigue, L 2015, 'Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis', Plant Molecular Biology Reporter, vol. 33, n.º 3, pp. 624-637. https://doi.org/10.1007/s11105-014-0782-5

Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis. / Herrera-Vásquez, Ariel; Carvallo, Loreto; Blanco, Francisca; Tobar, Mariola; Villarroel-Candia, Eva; Vicente-Carbajosa, Jesús; Salinas, Paula; Holuigue, Loreto.

En: Plant Molecular Biology Reporter, Vol. 33, N.º 3, 16.06.2015, p. 624-637.

Resultado de la investigación: Article

TY - JOUR

T1 - Transcriptional Control of Glutaredoxin GRXC9 Expression by a Salicylic Acid-Dependent and NPR1-Independent Pathway in Arabidopsis

AU - Herrera-Vásquez, Ariel

AU - Carvallo, Loreto

AU - Blanco, Francisca

AU - Tobar, Mariola

AU - Villarroel-Candia, Eva

AU - Vicente-Carbajosa, Jesús

AU - Salinas, Paula

AU - Holuigue, Loreto

PY - 2015/6/16

Y1 - 2015/6/16

N2 - Salicylic acid (SA) is a key hormone that mediates gene transcriptional reprogramming in the context of the defense response to stress. GRXC9, coding for a CC-type glutaredoxin from Arabidopsis, is an SA-responsive gene induced early and transiently by an NPR1-independent pathway. Here, we address the mechanism involved in this SA-dependent pathway, using GRXC9 as a model gene. We first established that GRXC9 expression is induced by UVB exposure through this pathway, validating its activation in a physiological stress condition. GRXC9 promoter analyses indicate that SA controls gene transcription through two activating sequence-1 (as-1)-like elements located in its proximal region. TGA2 and TGA3, but not TGA1, are constitutively bound to this promoter region. Accordingly, the transient recruitment of RNA polymerase II to the GRXC9 promoter, as well as the transient accumulation of gene transcripts detected in SA-treated WT plants, was abolished in a knockout mutant for the TGA class II factors. We conclude that constitutive binding of TGA2 is essential for controlling GRXC9 expression, while binding of TGA3 in a lesser extent contributes to this regulation. Finally, overexpression of GRXC9 indicates that the GRXC9 protein negatively controls its own gene expression, forming part of the complex bound to the as-1-containing promoter region. These findings are integrated in a model that explains how SA controls transcription of GRXC9 in the context of the defense response to stress.

AB - Salicylic acid (SA) is a key hormone that mediates gene transcriptional reprogramming in the context of the defense response to stress. GRXC9, coding for a CC-type glutaredoxin from Arabidopsis, is an SA-responsive gene induced early and transiently by an NPR1-independent pathway. Here, we address the mechanism involved in this SA-dependent pathway, using GRXC9 as a model gene. We first established that GRXC9 expression is induced by UVB exposure through this pathway, validating its activation in a physiological stress condition. GRXC9 promoter analyses indicate that SA controls gene transcription through two activating sequence-1 (as-1)-like elements located in its proximal region. TGA2 and TGA3, but not TGA1, are constitutively bound to this promoter region. Accordingly, the transient recruitment of RNA polymerase II to the GRXC9 promoter, as well as the transient accumulation of gene transcripts detected in SA-treated WT plants, was abolished in a knockout mutant for the TGA class II factors. We conclude that constitutive binding of TGA2 is essential for controlling GRXC9 expression, while binding of TGA3 in a lesser extent contributes to this regulation. Finally, overexpression of GRXC9 indicates that the GRXC9 protein negatively controls its own gene expression, forming part of the complex bound to the as-1-containing promoter region. These findings are integrated in a model that explains how SA controls transcription of GRXC9 in the context of the defense response to stress.

KW - as-1-like element

KW - Glutaredoxin GRXC9 (GRX480)

KW - NPR1-independent

KW - Salicylic acid

KW - TGA transcription factors

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U2 - 10.1007/s11105-014-0782-5

DO - 10.1007/s11105-014-0782-5

M3 - Article

VL - 33

SP - 624

EP - 637

JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

SN - 0735-9640

IS - 3

ER -