Resumen
A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer- acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5'-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer- acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat.
| Idioma original | English |
|---|---|
| Páginas (desde-hasta) | 598-609 |
| Número de páginas | 12 |
| Publicación | Journal of Cellular Biochemistry |
| Volumen | 75 |
| N.º | 4 |
| DOI | |
| Estado | Published - 15 dic 1999 |
Huella dactilar
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology
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Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms : Identification of two Pit-1 genes in the carp. / Kausel, Gudrun; Vera, María Inés; Martin, Rody San; Figueroa, Jaime; Molina, Alfredo; Muller, Marc; Martial, Joseph; Krauskopf, Manuel.
En: Journal of Cellular Biochemistry, Vol. 75, N.º 4, 15.12.1999, p. 598-609.Resultado de la investigación: Article
TY - JOUR
T1 - Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms
T2 - Identification of two Pit-1 genes in the carp
AU - Kausel, Gudrun
AU - Vera, María Inés
AU - Martin, Rody San
AU - Figueroa, Jaime
AU - Molina, Alfredo
AU - Muller, Marc
AU - Martial, Joseph
AU - Krauskopf, Manuel
PY - 1999/12/15
Y1 - 1999/12/15
N2 - A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer- acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5'-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer- acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat.
AB - A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer- acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5'-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer- acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat.
KW - Acclimatization
KW - Carp
KW - Fish
KW - Genomic organization
KW - Pit-1
KW - Pit-1 gene expression
KW - Seasonal control
UR - http://www.scopus.com/inward/record.url?scp=0033573297&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-4644(19991215)75:4<598::AID-JCB6>3.0.CO;2-J
DO - 10.1002/(SICI)1097-4644(19991215)75:4<598::AID-JCB6>3.0.CO;2-J
M3 - Article
C2 - 10572243
AN - SCOPUS:0033573297
VL - 75
SP - 598
EP - 609
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
SN - 0730-2312
IS - 4
ER -