Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: Identification of two Pit-1 genes in the carp

Gudrun Kausel, María Inés Vera, Rody San Martin, Jaime Figueroa, Alfredo Molina, Marc Muller, Joseph Martial, Manuel Krauskopf

Resultado de la investigación: Article

23 Citas (Scopus)

Resumen

A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer- acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5'-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer- acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat.

Idioma originalEnglish
Páginas (desde-hasta)598-609
Número de páginas12
PublicaciónJournal of Cellular Biochemistry
Volumen75
N.º4
DOI
EstadoPublished - 15 dic 1999

Huella dactilar

Transcription Factor Pit-1
Carps
Acclimatization
Transcription
Fish
Genes
Polymerase chain reaction
Messenger RNA
Fishes
Genetic Promoter Regions
Gene expression
Prolactin
Introns
Reverse Transcription
Exons
Polymerase Chain Reaction
Pituitary Gland
In Situ Hybridization
Ecosystem
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Citar esto

Kausel, Gudrun ; Vera, María Inés ; Martin, Rody San ; Figueroa, Jaime ; Molina, Alfredo ; Muller, Marc ; Martial, Joseph ; Krauskopf, Manuel. / Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms : Identification of two Pit-1 genes in the carp. En: Journal of Cellular Biochemistry. 1999 ; Vol. 75, N.º 4. pp. 598-609.
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abstract = "A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer- acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5'-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer- acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat.",
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Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms : Identification of two Pit-1 genes in the carp. / Kausel, Gudrun; Vera, María Inés; Martin, Rody San; Figueroa, Jaime; Molina, Alfredo; Muller, Marc; Martial, Joseph; Krauskopf, Manuel.

En: Journal of Cellular Biochemistry, Vol. 75, N.º 4, 15.12.1999, p. 598-609.

Resultado de la investigación: Article

TY - JOUR

T1 - Transcription factor Pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms

T2 - Identification of two Pit-1 genes in the carp

AU - Kausel, Gudrun

AU - Vera, María Inés

AU - Martin, Rody San

AU - Figueroa, Jaime

AU - Molina, Alfredo

AU - Muller, Marc

AU - Martial, Joseph

AU - Krauskopf, Manuel

PY - 1999/12/15

Y1 - 1999/12/15

N2 - A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer- acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5'-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer- acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat.

AB - A second Pit-1 gene in carp (Cyprinus carpio), including the complete structural gene and 1.1 kb of promoter region, was identified and completely sequenced. The exon-intron structure was determined, and reverse transcription-polymerase chain reaction (RT-PCR) experiments suggest that only one Pit-1 splice variant is present in carp pituitary. The effect of seasonal acclimatization on the extent of Pit-1 gene expression was studied in summer- and winter-acclimatized carp. Quantitative RT-PCR analysis revealed a clear increase of Pit-1 mRNA in the pituitaries from summer- acclimatized carp compared with the winter-adapted fish. In situ hybridization of pituitary gland sections with riboprobes representing the complete 5'-transactivating region of carp Pit-1 depicted a significantly higher Pit-1 mRNA level in the rostral pars distalis of the summer- acclimatized fish where prolactin is expressed in a manner that resembles the seasonal increase observed in the proximal pars distalis and the pars intermedia. The cell- and temporal-specific transcription of Pit-1 supports its role in the molecular mechanisms that underly the acclimatization process undergone by eurythermal fish as a result of the physical effects of seasonal changes on their habitat.

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KW - Carp

KW - Fish

KW - Genomic organization

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KW - Seasonal control

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