Tissue uptake of human sex hormone-binding globulin and its influence on ligand kinetics in the adult female rat

G. Noe, Y. C. Cheng, M. Dabike, H. B. Croxatto

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

28 Citas (Scopus)

Resumen

The distribution of human sex hormone-binding globulin (hSHBG) and its influence upon the kinetics of its ligands were assessed in the adult female rat, which lacks a comparable protein in serum. Purified hSHBG was administered i.v. to adult female rats as a single bolus. The plasma disappearance rate of immunoreactive hSHBG had one component with a half-life of 15 h. The estradiol (E2) binding activity of serum attributable to hSHBG was elevated 2-fold; during a continuous infusion of E2, hSHBG increased E2 serum levels above those of control infused animals. Treatment with hSHBG did not modify the plasma clearance of endogenous E2, but accelerated the disappearance rate of 5α-dihydrotestosterone (DHT). In animals injected with a tracer dose of radioactive steroids, pretreatment with hSHBG increased uterine and oviductal accumulation of E2- but not DHT-associated radioactivity. This effect was specific to some E2 target tissues since hSHBG did not alter the concentration of E2- or DHT-associated radioactivity in the hypophysis, liver, diaphragm, or brain. Treatment with anti-E2 antibodies, which elevated E2 binding activity in serum, decreased the accumulation of E2-associated radioactivity in uterus and oviduct. Immunofluorescent localization of hSHBG revealed intense labeling of the uterine and oviductal epithelium. We conclude that this foreign hormone- binding globulin introduced in serum at concentrations that have minimal circulating reservoir effect on E2 can reach intracellular domains and affect the concentration of this ligand in target tissues.

Idioma originalInglés
Páginas (desde-hasta)970-976
Número de páginas7
PublicaciónBiology of Reproduction
Volumen47
N.º6
DOI
EstadoPublicada - 1992

Áreas temáticas de ASJC Scopus

  • Medicina reproductiva
  • Biología celular

Huella

Profundice en los temas de investigación de 'Tissue uptake of human sex hormone-binding globulin and its influence on ligand kinetics in the adult female rat'. En conjunto forman una huella única.

Citar esto