Three different genes encode the iron-sulfur subunit of succinate dehydrogenase in Arabidopsis thaliana

Pablo Figueroa, Gabriel León, Alvaro Elorza, Loreto Holuigue, Xavier Jordana

Resultado de la investigación: Article

30 Citas (Scopus)

Resumen

The iron-sulfur protein is an essential component of mitochondrial complex II (succinate dehydrogenase, SDH), which is a functional enzyme of both the citric acid cycle and the respiratory, electron transport chain. This protein is encoded by a single-copy nuclear gene in mammals and fungi and by a mitochondrial gene in Rhodophyta and the protist Reclinomonas americana. In Arabidopsis thaliana, the homologous protein is now found to be encoded by three nuclear genes. Two genes (sdh2-1 and sdh2-2) likely arose from a relatively recent duplication event since they have similar structures, encode nearly identical proteins and show similar expression patterns. Both genes are interrupted by a single intron located at a conserved position. Expression was detected in all tissues analysed, with the highest steady-state mRNA levels found in flowers and inflorescences. In contrast, the third gene (sdh2-3) is interrupted by 4 introns, is expressed at a low level, and encodes a SDH2-3 protein which is only 67% similar to SDH2-1 and SDH2-2 and has a different N-terminal presequence. Interestingly, the proteins encoded by these three genes are probably functional because they are highly conserved compared with their homologues in other organisms. These proteins contain the cysteine motifs involved binding the three iron-sulfur clusters essential for electron transport. Furthermore, the three polypeptides are found to be imported into isolated plant mitochondria.

Idioma originalEnglish
Páginas (desde-hasta)241-250
Número de páginas10
PublicaciónPlant Molecular Biology
Volumen46
N.º2
DOI
EstadoPublished - 14 jul 2001

Huella dactilar

Succinate Dehydrogenase
Sulfur
Arabidopsis
sulfur
Iron
Arabidopsis thaliana
Genes
iron
Proteins
genes
Electron Transport
proteins
Introns
Respiratory Transport
introns
Iron-Sulfur Proteins
Rhodophyta
Inflorescence
sulfur proteins
Mitochondrial Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Citar esto

Figueroa, Pablo ; León, Gabriel ; Elorza, Alvaro ; Holuigue, Loreto ; Jordana, Xavier. / Three different genes encode the iron-sulfur subunit of succinate dehydrogenase in Arabidopsis thaliana. En: Plant Molecular Biology. 2001 ; Vol. 46, N.º 2. pp. 241-250.
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abstract = "The iron-sulfur protein is an essential component of mitochondrial complex II (succinate dehydrogenase, SDH), which is a functional enzyme of both the citric acid cycle and the respiratory, electron transport chain. This protein is encoded by a single-copy nuclear gene in mammals and fungi and by a mitochondrial gene in Rhodophyta and the protist Reclinomonas americana. In Arabidopsis thaliana, the homologous protein is now found to be encoded by three nuclear genes. Two genes (sdh2-1 and sdh2-2) likely arose from a relatively recent duplication event since they have similar structures, encode nearly identical proteins and show similar expression patterns. Both genes are interrupted by a single intron located at a conserved position. Expression was detected in all tissues analysed, with the highest steady-state mRNA levels found in flowers and inflorescences. In contrast, the third gene (sdh2-3) is interrupted by 4 introns, is expressed at a low level, and encodes a SDH2-3 protein which is only 67{\%} similar to SDH2-1 and SDH2-2 and has a different N-terminal presequence. Interestingly, the proteins encoded by these three genes are probably functional because they are highly conserved compared with their homologues in other organisms. These proteins contain the cysteine motifs involved binding the three iron-sulfur clusters essential for electron transport. Furthermore, the three polypeptides are found to be imported into isolated plant mitochondria.",
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Three different genes encode the iron-sulfur subunit of succinate dehydrogenase in Arabidopsis thaliana. / Figueroa, Pablo; León, Gabriel; Elorza, Alvaro; Holuigue, Loreto; Jordana, Xavier.

En: Plant Molecular Biology, Vol. 46, N.º 2, 14.07.2001, p. 241-250.

Resultado de la investigación: Article

TY - JOUR

T1 - Three different genes encode the iron-sulfur subunit of succinate dehydrogenase in Arabidopsis thaliana

AU - Figueroa, Pablo

AU - León, Gabriel

AU - Elorza, Alvaro

AU - Holuigue, Loreto

AU - Jordana, Xavier

PY - 2001/7/14

Y1 - 2001/7/14

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AB - The iron-sulfur protein is an essential component of mitochondrial complex II (succinate dehydrogenase, SDH), which is a functional enzyme of both the citric acid cycle and the respiratory, electron transport chain. This protein is encoded by a single-copy nuclear gene in mammals and fungi and by a mitochondrial gene in Rhodophyta and the protist Reclinomonas americana. In Arabidopsis thaliana, the homologous protein is now found to be encoded by three nuclear genes. Two genes (sdh2-1 and sdh2-2) likely arose from a relatively recent duplication event since they have similar structures, encode nearly identical proteins and show similar expression patterns. Both genes are interrupted by a single intron located at a conserved position. Expression was detected in all tissues analysed, with the highest steady-state mRNA levels found in flowers and inflorescences. In contrast, the third gene (sdh2-3) is interrupted by 4 introns, is expressed at a low level, and encodes a SDH2-3 protein which is only 67% similar to SDH2-1 and SDH2-2 and has a different N-terminal presequence. Interestingly, the proteins encoded by these three genes are probably functional because they are highly conserved compared with their homologues in other organisms. These proteins contain the cysteine motifs involved binding the three iron-sulfur clusters essential for electron transport. Furthermore, the three polypeptides are found to be imported into isolated plant mitochondria.

KW - Arabidopsis thaliana

KW - Iron-sulfur subunit

KW - Mitochondrial targeting

KW - Plant mitochondria

KW - Respiratory complex II

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