The use of insertion sequences to analyse gene function in Thiobacillus ferrooxidans: A case study involving cytochrome c-type biogenesis proteins in iron oxidation

D. S. Holmes, E. Jedlicki, M. E. Cabrejos, S. Bueno, M. Guacucano, C. Inostroza, G. Levican, P. Varela, E. Garcia

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

With DNA sequence information available for Thiobacillus ferrooxidans it will become much easier to understand its physiology and genetics. However, based on DNA sequence information alone a number of its genes, especially those involved in the more unusual or specific functions of the microorganism such as iron oxidation, could remain without a clear assignment of function. We show how naturally occurring insertion sequences can be used to generate mutants by knocking out gene function and how these knock-out mutants can be analyzed to reveal the gene involved in the mutation. An iron oxidizing mutant of T. ferrooxidans ATCC19859 is shown to contain a copy of the insertion sequence IST1 in a gene, which we term resB, that is probably involved in the biogenesis of a c-type cytochrome. Downstream of resB, and perhaps part of the same transcriptional unit, is another gene, resC, also probably involved in the biogenesis of a c-type cytochrome. Some of the predicted properties of these biogenesis proteins and their evolutionary relations are described. The target cytochrome(s) of the putative cytochrome-c type biogenesis proteins remains unknown, although just upstream of resB is a gene potentially encoding a cytochrome cl, making it a likely candidate as the target cytochrome. The target cytochrome is essential for growth on iron but not for growth on thiosulfate and may prove specific for the pathway of energy transduction and/or electron flow during iron oxidation.

Idioma originalEnglish
Páginas (desde-hasta)139-147
Número de páginas9
PublicaciónProcess Metallurgy
Volumen9
N.ºC
DOI
EstadoPublished - 1 dic 1999

Huella dactilar

cytochrome
Genes
Iron
Proteins
oxidation
iron
Oxidation
protein
gene
DNA sequences
DNA
thiosulfate
Gene encoding
physiology
Physiology
mutation
microorganism
Microorganisms
electron
Electrons

ASJC Scopus subject areas

  • Geotechnical Engineering and Engineering Geology
  • Industrial and Manufacturing Engineering

Citar esto

Holmes, D. S. ; Jedlicki, E. ; Cabrejos, M. E. ; Bueno, S. ; Guacucano, M. ; Inostroza, C. ; Levican, G. ; Varela, P. ; Garcia, E. / The use of insertion sequences to analyse gene function in Thiobacillus ferrooxidans : A case study involving cytochrome c-type biogenesis proteins in iron oxidation. En: Process Metallurgy. 1999 ; Vol. 9, N.º C. pp. 139-147.
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abstract = "With DNA sequence information available for Thiobacillus ferrooxidans it will become much easier to understand its physiology and genetics. However, based on DNA sequence information alone a number of its genes, especially those involved in the more unusual or specific functions of the microorganism such as iron oxidation, could remain without a clear assignment of function. We show how naturally occurring insertion sequences can be used to generate mutants by knocking out gene function and how these knock-out mutants can be analyzed to reveal the gene involved in the mutation. An iron oxidizing mutant of T. ferrooxidans ATCC19859 is shown to contain a copy of the insertion sequence IST1 in a gene, which we term resB, that is probably involved in the biogenesis of a c-type cytochrome. Downstream of resB, and perhaps part of the same transcriptional unit, is another gene, resC, also probably involved in the biogenesis of a c-type cytochrome. Some of the predicted properties of these biogenesis proteins and their evolutionary relations are described. The target cytochrome(s) of the putative cytochrome-c type biogenesis proteins remains unknown, although just upstream of resB is a gene potentially encoding a cytochrome cl, making it a likely candidate as the target cytochrome. The target cytochrome is essential for growth on iron but not for growth on thiosulfate and may prove specific for the pathway of energy transduction and/or electron flow during iron oxidation.",
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Holmes, DS, Jedlicki, E, Cabrejos, ME, Bueno, S, Guacucano, M, Inostroza, C, Levican, G, Varela, P & Garcia, E 1999, 'The use of insertion sequences to analyse gene function in Thiobacillus ferrooxidans: A case study involving cytochrome c-type biogenesis proteins in iron oxidation', Process Metallurgy, vol. 9, n.º C, pp. 139-147. https://doi.org/10.1016/S1572-4409(99)80102-3

The use of insertion sequences to analyse gene function in Thiobacillus ferrooxidans : A case study involving cytochrome c-type biogenesis proteins in iron oxidation. / Holmes, D. S.; Jedlicki, E.; Cabrejos, M. E.; Bueno, S.; Guacucano, M.; Inostroza, C.; Levican, G.; Varela, P.; Garcia, E.

En: Process Metallurgy, Vol. 9, N.º C, 01.12.1999, p. 139-147.

Resultado de la investigación: Article

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T1 - The use of insertion sequences to analyse gene function in Thiobacillus ferrooxidans

T2 - A case study involving cytochrome c-type biogenesis proteins in iron oxidation

AU - Holmes, D. S.

AU - Jedlicki, E.

AU - Cabrejos, M. E.

AU - Bueno, S.

AU - Guacucano, M.

AU - Inostroza, C.

AU - Levican, G.

AU - Varela, P.

AU - Garcia, E.

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N2 - With DNA sequence information available for Thiobacillus ferrooxidans it will become much easier to understand its physiology and genetics. However, based on DNA sequence information alone a number of its genes, especially those involved in the more unusual or specific functions of the microorganism such as iron oxidation, could remain without a clear assignment of function. We show how naturally occurring insertion sequences can be used to generate mutants by knocking out gene function and how these knock-out mutants can be analyzed to reveal the gene involved in the mutation. An iron oxidizing mutant of T. ferrooxidans ATCC19859 is shown to contain a copy of the insertion sequence IST1 in a gene, which we term resB, that is probably involved in the biogenesis of a c-type cytochrome. Downstream of resB, and perhaps part of the same transcriptional unit, is another gene, resC, also probably involved in the biogenesis of a c-type cytochrome. Some of the predicted properties of these biogenesis proteins and their evolutionary relations are described. The target cytochrome(s) of the putative cytochrome-c type biogenesis proteins remains unknown, although just upstream of resB is a gene potentially encoding a cytochrome cl, making it a likely candidate as the target cytochrome. The target cytochrome is essential for growth on iron but not for growth on thiosulfate and may prove specific for the pathway of energy transduction and/or electron flow during iron oxidation.

AB - With DNA sequence information available for Thiobacillus ferrooxidans it will become much easier to understand its physiology and genetics. However, based on DNA sequence information alone a number of its genes, especially those involved in the more unusual or specific functions of the microorganism such as iron oxidation, could remain without a clear assignment of function. We show how naturally occurring insertion sequences can be used to generate mutants by knocking out gene function and how these knock-out mutants can be analyzed to reveal the gene involved in the mutation. An iron oxidizing mutant of T. ferrooxidans ATCC19859 is shown to contain a copy of the insertion sequence IST1 in a gene, which we term resB, that is probably involved in the biogenesis of a c-type cytochrome. Downstream of resB, and perhaps part of the same transcriptional unit, is another gene, resC, also probably involved in the biogenesis of a c-type cytochrome. Some of the predicted properties of these biogenesis proteins and their evolutionary relations are described. The target cytochrome(s) of the putative cytochrome-c type biogenesis proteins remains unknown, although just upstream of resB is a gene potentially encoding a cytochrome cl, making it a likely candidate as the target cytochrome. The target cytochrome is essential for growth on iron but not for growth on thiosulfate and may prove specific for the pathway of energy transduction and/or electron flow during iron oxidation.

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