The Unusual Acid-Accumulating Behavior during Ripening of Cherimoya (Annona cherimola Mill.) is Linked to Changes in Transcription and Enzyme Activity Related to Citric and Malic Acid Metabolism

Mauricio González-Agüero, Luis Tejerina Pardo, María Sofía Zamudio, Carolina Contreras, Pedro Undurraga, Bruno G. Defilippi

Resultado de la investigación: Article

Resumen

Cherimoya (Annona cherimola Mill.) is a subtropical fruit characterized by a significant increase in organic acid levels during ripening, making it an interesting model for studying the relationship between acidity and fruit flavor. In this work, we focused on understanding the balance between the concentration of organic acids and the gene expression and activity of enzymes involved in the synthesis and degradation of these metabolites during the development and ripening of cherimoya cv. "Concha Lisa". Our results showed an early accumulation of citric acid and other changes associated with the accumulation of transcripts encoding citrate catabolism enzymes. During ripening, a 2-fold increase in malic acid and a 6-fold increase in citric acid were detected. By comparing the contents of these compounds with gene expression and enzymatic activity levels, we determined that cytoplasmic NAD-dependent malate dehydrogenase (cyNAD-MDH) and mitochondrial citrate synthase (mCS) play important regulatory roles in the malic and citric acid biosynthetic pathways.

Idioma originalEnglish
PublicaciónMolecules (Basel, Switzerland)
Volumen21
N.º5
DOI
EstadoPublished - 25 abr 2016
Publicado de forma externa

Huella dactilar

Annona
enzyme activity
citric acid
Enzyme activity
metabolism
Transcription
Metabolism
Citric Acid
acids
Acids
fruits
gene expression
Organic acids
Enzymes
citrates
Fruits
Gene expression
enzymes
Fruit
catabolism

ASJC Scopus subject areas

  • Medicine(all)
  • Organic Chemistry

Citar esto

González-Agüero, Mauricio ; Tejerina Pardo, Luis ; Zamudio, María Sofía ; Contreras, Carolina ; Undurraga, Pedro ; Defilippi, Bruno G. / The Unusual Acid-Accumulating Behavior during Ripening of Cherimoya (Annona cherimola Mill.) is Linked to Changes in Transcription and Enzyme Activity Related to Citric and Malic Acid Metabolism. En: Molecules (Basel, Switzerland). 2016 ; Vol. 21, N.º 5.
@article{08dc7a7d266d454b9580a37aee7b62a2,
title = "The Unusual Acid-Accumulating Behavior during Ripening of Cherimoya (Annona cherimola Mill.) is Linked to Changes in Transcription and Enzyme Activity Related to Citric and Malic Acid Metabolism",
abstract = "Cherimoya (Annona cherimola Mill.) is a subtropical fruit characterized by a significant increase in organic acid levels during ripening, making it an interesting model for studying the relationship between acidity and fruit flavor. In this work, we focused on understanding the balance between the concentration of organic acids and the gene expression and activity of enzymes involved in the synthesis and degradation of these metabolites during the development and ripening of cherimoya cv. {"}Concha Lisa{"}. Our results showed an early accumulation of citric acid and other changes associated with the accumulation of transcripts encoding citrate catabolism enzymes. During ripening, a 2-fold increase in malic acid and a 6-fold increase in citric acid were detected. By comparing the contents of these compounds with gene expression and enzymatic activity levels, we determined that cytoplasmic NAD-dependent malate dehydrogenase (cyNAD-MDH) and mitochondrial citrate synthase (mCS) play important regulatory roles in the malic and citric acid biosynthetic pathways.",
keywords = "Annona cherimola, gene expression, organic acids",
author = "Mauricio Gonz{\'a}lez-Ag{\"u}ero and {Tejerina Pardo}, Luis and Zamudio, {Mar{\'i}a Sof{\'i}a} and Carolina Contreras and Pedro Undurraga and Defilippi, {Bruno G.}",
year = "2016",
month = "4",
day = "25",
doi = "10.3390/molecules21050398",
language = "English",
volume = "21",
journal = "Molecules",
issn = "1420-3049",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "5",

}

The Unusual Acid-Accumulating Behavior during Ripening of Cherimoya (Annona cherimola Mill.) is Linked to Changes in Transcription and Enzyme Activity Related to Citric and Malic Acid Metabolism. / González-Agüero, Mauricio; Tejerina Pardo, Luis; Zamudio, María Sofía; Contreras, Carolina; Undurraga, Pedro; Defilippi, Bruno G.

En: Molecules (Basel, Switzerland), Vol. 21, N.º 5, 25.04.2016.

Resultado de la investigación: Article

TY - JOUR

T1 - The Unusual Acid-Accumulating Behavior during Ripening of Cherimoya (Annona cherimola Mill.) is Linked to Changes in Transcription and Enzyme Activity Related to Citric and Malic Acid Metabolism

AU - González-Agüero, Mauricio

AU - Tejerina Pardo, Luis

AU - Zamudio, María Sofía

AU - Contreras, Carolina

AU - Undurraga, Pedro

AU - Defilippi, Bruno G.

PY - 2016/4/25

Y1 - 2016/4/25

N2 - Cherimoya (Annona cherimola Mill.) is a subtropical fruit characterized by a significant increase in organic acid levels during ripening, making it an interesting model for studying the relationship between acidity and fruit flavor. In this work, we focused on understanding the balance between the concentration of organic acids and the gene expression and activity of enzymes involved in the synthesis and degradation of these metabolites during the development and ripening of cherimoya cv. "Concha Lisa". Our results showed an early accumulation of citric acid and other changes associated with the accumulation of transcripts encoding citrate catabolism enzymes. During ripening, a 2-fold increase in malic acid and a 6-fold increase in citric acid were detected. By comparing the contents of these compounds with gene expression and enzymatic activity levels, we determined that cytoplasmic NAD-dependent malate dehydrogenase (cyNAD-MDH) and mitochondrial citrate synthase (mCS) play important regulatory roles in the malic and citric acid biosynthetic pathways.

AB - Cherimoya (Annona cherimola Mill.) is a subtropical fruit characterized by a significant increase in organic acid levels during ripening, making it an interesting model for studying the relationship between acidity and fruit flavor. In this work, we focused on understanding the balance between the concentration of organic acids and the gene expression and activity of enzymes involved in the synthesis and degradation of these metabolites during the development and ripening of cherimoya cv. "Concha Lisa". Our results showed an early accumulation of citric acid and other changes associated with the accumulation of transcripts encoding citrate catabolism enzymes. During ripening, a 2-fold increase in malic acid and a 6-fold increase in citric acid were detected. By comparing the contents of these compounds with gene expression and enzymatic activity levels, we determined that cytoplasmic NAD-dependent malate dehydrogenase (cyNAD-MDH) and mitochondrial citrate synthase (mCS) play important regulatory roles in the malic and citric acid biosynthetic pathways.

KW - Annona cherimola

KW - gene expression

KW - organic acids

UR - http://www.scopus.com/inward/record.url?scp=85014942424&partnerID=8YFLogxK

U2 - 10.3390/molecules21050398

DO - 10.3390/molecules21050398

M3 - Article

C2 - 27120592

VL - 21

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 5

ER -