The transcription factor SlyA from Salmonella Typhimurium regulates genes in response to hydrogen peroxide and sodium hypochlorite

Carolina E. Cabezas, Alan C. Briones, Camila Aguirre, Coral Pardo-Esté, Juan Castro-Severyn, César R. Salinas, María S. Baquedano, Alejandro A. Hidalgo, Juan A. Fuentes, Eduardo H. Morales, Claudio A. Meneses, Eduardo Castro-Nallar, Claudia Paz Saavedra

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

Salmonella Typhimurium is an intracellular pathogen that is capable of generating systemic fever in a murine model. Over the course of the infection, Salmonella faces different kinds of stressors, including harmful reactive oxygen species (ROS). Various defence mechanisms enable Salmonella to successfully complete the infective process in the presence of such stressors. The transcriptional factor SlyA is involved in the oxidative stress response and invasion of murine macrophages. We evaluated the role of SlyA in response to H2O2 and NaOCl and found an increase of slyA expression upon exposure to these toxics. However, the SlyA target genes and the molecular mechanisms by which they influence the infective process are unknown. We hypothesised that SlyA regulates the expression of genes required for ROS resistance, metabolism, or virulence under oxidative stress conditions. Transcriptional profiling in wild type and ΔslyA strains confirmed that SlyA regulates the expression of several genes involved in virulence [sopD (STM14_3550), sopE2 (STM14_2244), hilA (STM14_3475)] and central metabolism [kgtP (STM14_3252), fruK (STM14_2722), glpA (STM14_2819)] in response to H2O2 and NaOCl. These findings were corroborated by functional assay and transcriptional fusion assays using GFP. DNA-protein interaction assays showed that SlyA regulates these genes through direct interaction with their promoter regions.

Idioma originalEnglish
PublicaciónResearch in Microbiology
DOI
EstadoAccepted/In press - 1 ene 2018

Huella dactilar

Sodium Hypochlorite
Salmonella typhimurium
Hydrogen Peroxide
Virulence
Reactive Oxygen Species
Oxidative Stress
Transcription Factors
Gene Expression
Salmonella Infections
Poisons
Genetic Promoter Regions
Salmonella
Genes
Fever
Macrophages
DNA
Proteins

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Citar esto

Cabezas, Carolina E. ; Briones, Alan C. ; Aguirre, Camila ; Pardo-Esté, Coral ; Castro-Severyn, Juan ; Salinas, César R. ; Baquedano, María S. ; Hidalgo, Alejandro A. ; Fuentes, Juan A. ; Morales, Eduardo H. ; Meneses, Claudio A. ; Castro-Nallar, Eduardo ; Saavedra, Claudia Paz. / The transcription factor SlyA from Salmonella Typhimurium regulates genes in response to hydrogen peroxide and sodium hypochlorite. En: Research in Microbiology. 2018.
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title = "The transcription factor SlyA from Salmonella Typhimurium regulates genes in response to hydrogen peroxide and sodium hypochlorite",
abstract = "Salmonella Typhimurium is an intracellular pathogen that is capable of generating systemic fever in a murine model. Over the course of the infection, Salmonella faces different kinds of stressors, including harmful reactive oxygen species (ROS). Various defence mechanisms enable Salmonella to successfully complete the infective process in the presence of such stressors. The transcriptional factor SlyA is involved in the oxidative stress response and invasion of murine macrophages. We evaluated the role of SlyA in response to H2O2 and NaOCl and found an increase of slyA expression upon exposure to these toxics. However, the SlyA target genes and the molecular mechanisms by which they influence the infective process are unknown. We hypothesised that SlyA regulates the expression of genes required for ROS resistance, metabolism, or virulence under oxidative stress conditions. Transcriptional profiling in wild type and ΔslyA strains confirmed that SlyA regulates the expression of several genes involved in virulence [sopD (STM14_3550), sopE2 (STM14_2244), hilA (STM14_3475)] and central metabolism [kgtP (STM14_3252), fruK (STM14_2722), glpA (STM14_2819)] in response to H2O2 and NaOCl. These findings were corroborated by functional assay and transcriptional fusion assays using GFP. DNA-protein interaction assays showed that SlyA regulates these genes through direct interaction with their promoter regions.",
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The transcription factor SlyA from Salmonella Typhimurium regulates genes in response to hydrogen peroxide and sodium hypochlorite. / Cabezas, Carolina E.; Briones, Alan C.; Aguirre, Camila; Pardo-Esté, Coral; Castro-Severyn, Juan; Salinas, César R.; Baquedano, María S.; Hidalgo, Alejandro A.; Fuentes, Juan A.; Morales, Eduardo H.; Meneses, Claudio A.; Castro-Nallar, Eduardo; Saavedra, Claudia Paz.

En: Research in Microbiology, 01.01.2018.

Resultado de la investigación: Article

TY - JOUR

T1 - The transcription factor SlyA from Salmonella Typhimurium regulates genes in response to hydrogen peroxide and sodium hypochlorite

AU - Cabezas, Carolina E.

AU - Briones, Alan C.

AU - Aguirre, Camila

AU - Pardo-Esté, Coral

AU - Castro-Severyn, Juan

AU - Salinas, César R.

AU - Baquedano, María S.

AU - Hidalgo, Alejandro A.

AU - Fuentes, Juan A.

AU - Morales, Eduardo H.

AU - Meneses, Claudio A.

AU - Castro-Nallar, Eduardo

AU - Saavedra, Claudia Paz

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Salmonella Typhimurium is an intracellular pathogen that is capable of generating systemic fever in a murine model. Over the course of the infection, Salmonella faces different kinds of stressors, including harmful reactive oxygen species (ROS). Various defence mechanisms enable Salmonella to successfully complete the infective process in the presence of such stressors. The transcriptional factor SlyA is involved in the oxidative stress response and invasion of murine macrophages. We evaluated the role of SlyA in response to H2O2 and NaOCl and found an increase of slyA expression upon exposure to these toxics. However, the SlyA target genes and the molecular mechanisms by which they influence the infective process are unknown. We hypothesised that SlyA regulates the expression of genes required for ROS resistance, metabolism, or virulence under oxidative stress conditions. Transcriptional profiling in wild type and ΔslyA strains confirmed that SlyA regulates the expression of several genes involved in virulence [sopD (STM14_3550), sopE2 (STM14_2244), hilA (STM14_3475)] and central metabolism [kgtP (STM14_3252), fruK (STM14_2722), glpA (STM14_2819)] in response to H2O2 and NaOCl. These findings were corroborated by functional assay and transcriptional fusion assays using GFP. DNA-protein interaction assays showed that SlyA regulates these genes through direct interaction with their promoter regions.

AB - Salmonella Typhimurium is an intracellular pathogen that is capable of generating systemic fever in a murine model. Over the course of the infection, Salmonella faces different kinds of stressors, including harmful reactive oxygen species (ROS). Various defence mechanisms enable Salmonella to successfully complete the infective process in the presence of such stressors. The transcriptional factor SlyA is involved in the oxidative stress response and invasion of murine macrophages. We evaluated the role of SlyA in response to H2O2 and NaOCl and found an increase of slyA expression upon exposure to these toxics. However, the SlyA target genes and the molecular mechanisms by which they influence the infective process are unknown. We hypothesised that SlyA regulates the expression of genes required for ROS resistance, metabolism, or virulence under oxidative stress conditions. Transcriptional profiling in wild type and ΔslyA strains confirmed that SlyA regulates the expression of several genes involved in virulence [sopD (STM14_3550), sopE2 (STM14_2244), hilA (STM14_3475)] and central metabolism [kgtP (STM14_3252), fruK (STM14_2722), glpA (STM14_2819)] in response to H2O2 and NaOCl. These findings were corroborated by functional assay and transcriptional fusion assays using GFP. DNA-protein interaction assays showed that SlyA regulates these genes through direct interaction with their promoter regions.

KW - Gene regulation

KW - Oxidative stress

KW - RNAseq

KW - ROS

KW - Salmonella

KW - SlyA

UR - http://www.scopus.com/inward/record.url?scp=85048142053&partnerID=8YFLogxK

U2 - 10.1016/j.resmic.2018.04.003

DO - 10.1016/j.resmic.2018.04.003

M3 - Article

AN - SCOPUS:85048142053

JO - Research in Microbiology

JF - Research in Microbiology

SN - 0923-2508

ER -