The role of the rodent insula in anxiety

Maxs Méndez-Ruette, Sergio Linsambarth, Rodrigo Moraga-Amaro, Daisy Quintana-Donoso, Luis Méndez, Giovanni Tamburini, Francisca Cornejo, Rodrigo F. Torres, Jimmy Stehberg

Resultado de la investigación: Article

Resumen

The human insula has been consistently reported to be overactivated in all anxiety disorders, activation which has been suggested to be proportional to the level of anxiety and shown to decrease with effective anxiolytic treatment. Nonetheless, studies evaluating the direct role of the insula in anxiety are lacking. Here, we set out to investigate the role of the rodent insula in anxiety by either inactivating different insular regions via microinjections of glutamatergic AMPA receptor antagonist CNQX or activating them by microinjection of GABA receptor antagonist bicuculline in rats, before measuring anxiety-like behavior using the elevated plus maze. Inactivation of caudal and medial insular regions induced anxiogenic effects, while their activation induced anxiolytic effects. In contrast, inactivation of more rostral areas induced anxiolytic effects and their activation, anxiogenic effects. These results suggest that the insula in the rat has a role in the modulation of anxiety-like behavior in rats, showing regional differences; rostral regions have an anxiogenic role, while medial and caudal regions have an anxiolytic role, with a transition area around bregma +0.5. The present study suggests that the insula has a direct role in anxiety.

Idioma originalEnglish
Número de artículo330
PublicaciónFrontiers in Physiology
Volumen10
N.ºMAR
DOI
EstadoPublished - 1 ene 2019

Huella dactilar

Rodentia
Anxiety
Anti-Anxiety Agents
Microinjections
GABA Antagonists
6-Cyano-7-nitroquinoxaline-2,3-dione
AMPA Receptors
Bicuculline
Anxiety Disorders
Therapeutics

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Citar esto

Méndez-Ruette, M., Linsambarth, S., Moraga-Amaro, R., Quintana-Donoso, D., Méndez, L., Tamburini, G., ... Stehberg, J. (2019). The role of the rodent insula in anxiety. Frontiers in Physiology, 10(MAR), [330]. https://doi.org/10.3389/fphys.2019.00330
Méndez-Ruette, Maxs ; Linsambarth, Sergio ; Moraga-Amaro, Rodrigo ; Quintana-Donoso, Daisy ; Méndez, Luis ; Tamburini, Giovanni ; Cornejo, Francisca ; Torres, Rodrigo F. ; Stehberg, Jimmy. / The role of the rodent insula in anxiety. En: Frontiers in Physiology. 2019 ; Vol. 10, N.º MAR.
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Méndez-Ruette, M, Linsambarth, S, Moraga-Amaro, R, Quintana-Donoso, D, Méndez, L, Tamburini, G, Cornejo, F, Torres, RF & Stehberg, J 2019, 'The role of the rodent insula in anxiety', Frontiers in Physiology, vol. 10, n.º MAR, 330. https://doi.org/10.3389/fphys.2019.00330

The role of the rodent insula in anxiety. / Méndez-Ruette, Maxs; Linsambarth, Sergio; Moraga-Amaro, Rodrigo; Quintana-Donoso, Daisy; Méndez, Luis; Tamburini, Giovanni; Cornejo, Francisca; Torres, Rodrigo F.; Stehberg, Jimmy.

En: Frontiers in Physiology, Vol. 10, N.º MAR, 330, 01.01.2019.

Resultado de la investigación: Article

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T1 - The role of the rodent insula in anxiety

AU - Méndez-Ruette, Maxs

AU - Linsambarth, Sergio

AU - Moraga-Amaro, Rodrigo

AU - Quintana-Donoso, Daisy

AU - Méndez, Luis

AU - Tamburini, Giovanni

AU - Cornejo, Francisca

AU - Torres, Rodrigo F.

AU - Stehberg, Jimmy

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The human insula has been consistently reported to be overactivated in all anxiety disorders, activation which has been suggested to be proportional to the level of anxiety and shown to decrease with effective anxiolytic treatment. Nonetheless, studies evaluating the direct role of the insula in anxiety are lacking. Here, we set out to investigate the role of the rodent insula in anxiety by either inactivating different insular regions via microinjections of glutamatergic AMPA receptor antagonist CNQX or activating them by microinjection of GABA receptor antagonist bicuculline in rats, before measuring anxiety-like behavior using the elevated plus maze. Inactivation of caudal and medial insular regions induced anxiogenic effects, while their activation induced anxiolytic effects. In contrast, inactivation of more rostral areas induced anxiolytic effects and their activation, anxiogenic effects. These results suggest that the insula in the rat has a role in the modulation of anxiety-like behavior in rats, showing regional differences; rostral regions have an anxiogenic role, while medial and caudal regions have an anxiolytic role, with a transition area around bregma +0.5. The present study suggests that the insula has a direct role in anxiety.

AB - The human insula has been consistently reported to be overactivated in all anxiety disorders, activation which has been suggested to be proportional to the level of anxiety and shown to decrease with effective anxiolytic treatment. Nonetheless, studies evaluating the direct role of the insula in anxiety are lacking. Here, we set out to investigate the role of the rodent insula in anxiety by either inactivating different insular regions via microinjections of glutamatergic AMPA receptor antagonist CNQX or activating them by microinjection of GABA receptor antagonist bicuculline in rats, before measuring anxiety-like behavior using the elevated plus maze. Inactivation of caudal and medial insular regions induced anxiogenic effects, while their activation induced anxiolytic effects. In contrast, inactivation of more rostral areas induced anxiolytic effects and their activation, anxiogenic effects. These results suggest that the insula in the rat has a role in the modulation of anxiety-like behavior in rats, showing regional differences; rostral regions have an anxiogenic role, while medial and caudal regions have an anxiolytic role, with a transition area around bregma +0.5. The present study suggests that the insula has a direct role in anxiety.

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Méndez-Ruette M, Linsambarth S, Moraga-Amaro R, Quintana-Donoso D, Méndez L, Tamburini G y otros. The role of the rodent insula in anxiety. Frontiers in Physiology. 2019 ene 1;10(MAR). 330. https://doi.org/10.3389/fphys.2019.00330