The proteome of biologically active membrane vesicles from Piscirickettsia salmonis LF-89 type strain identifies plasmid-encoded putative toxins

Cristian Oliver, Mauricio A. Hernández, Julia I. Tandberg, Karla N. Valenzuela, Leidy X. Lagos, Ronie E. Haro, Sánchez Patricio Sánchez, Pamela A. Ruiz, Constanza Sanhueza-Oyarzún, Marcos A. Cortés, María T. Villar, Antonio Artigues, Hanne C. Winther-Larsen, Ruben Avendaño-Herrera, Alejandro J. Yáñez

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

15 Citas (Scopus)

Resumen

Piscirickettsia salmonis is the predominant bacterial pathogen affecting the Chilean salmonid industry. This bacterium is the etiological agent of piscirickettsiosis, a significant fish disease. Membrane vesicles (MVs) released by P. salmonis deliver several virulence factors to host cells. To improve on existing knowledge for the pathogenicity-associated functions of P. salmonis MVs, we studied the proteome of purified MVs from the P. salmonis LF-89 type strain using multidimensional protein identification technology. Initially, the cytotoxicity of different MV concentration purified from P. salmonis LF-89 was confirmed in an in vivo adult zebrafish infection model. The cumulative mortality of zebrafish injected with MVs showed a dose-dependent pattern. Analyses identified 452 proteins of different subcellular origins; most of them were associated with the cytoplasmic compartment and were mainly related to key functions for pathogen survival. Interestingly, previously unidentified putative virulence-related proteins were identified in P. salmonis MVs, such as outer membrane porin F and hemolysin. Additionally, five amino acid sequences corresponding to the Bordetella pertussis toxin subunit 1 and two amino acid sequences corresponding to the heat-labile enterotoxin alpha chain of Escherichia coli were located in the P. salmonis MV proteome. Curiously, these putative toxins were located in a plasmid region of P. salmonis LF-89. Based on the identified proteins, we propose that the protein composition of P. salmonis LF-89 MVs could reflect total protein characteristics of this P. salmonis type strain.

Idioma originalInglés
Número de artículo420
PublicaciónFrontiers in cellular and infection microbiology
Volumen7
N.ºSEP
DOI
EstadoPublicada - 28 sep 2017

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Inmunología
  • Microbiología (médica)
  • Enfermedades infecciosas

Huella Profundice en los temas de investigación de 'The proteome of biologically active membrane vesicles from Piscirickettsia salmonis LF-89 type strain identifies plasmid-encoded putative toxins'. En conjunto forman una huella única.

Citar esto