The OXPHOS supercomplex assembly factor HIG2A responds to changes in energetic metabolism and cell cycle

Celia Salazar, Alvaro A. Elorza, Glenda Cofre, Paula Ruiz-Hincapie, Orian Shirihai, Lina María Ruiz

Resultado de la investigación: Article

2 Citas (Scopus)

Resumen

HIG2A promotes cell survival under hypoxia and mediates the assembly of complex III and complex IV into respiratory chain supercomplexes. In the present study, we show that human HIGD2A and mouse Higd2a gene expressions are regulated by hypoxia, glucose, and the cell cycle-related transcription factor E2F1. The latter was found to bind the promoter region of HIGD2A. Differential expression of the HIGD2A gene was found in C57BL/6 mice in relation to tissue and age. Besides, the silencing of HIGD2A evidenced the modulation of mitochondrial dynamics proteins namely, OPA1 as a fusion protein increases, while FIS1, a fission protein, decreases. Besides, the mitochondrial membrane potential (ΔΨm) increased. The protein HIG2A is localized in the mitochondria and nucleus. Moreover, we observed that the HIG2A protein interacts with OPA1. Changes in oxygen concentration, glucose availability, and cell cycle regulate HIGD2A expression. Alterations in HIGD2A expression are associated with changes in mitochondrial physiology.

Idioma originalEnglish
PublicaciónJournal of Cellular Physiology
DOI
EstadoPublished - 1 ene 2019

Huella dactilar

Metabolism
Cell Cycle
Cells
Proteins
E2F1 Transcription Factor
Mitochondrial Dynamics
Gene Expression
Glucose
Mitochondria
Mitochondrial Membrane Potential
Electron Transport Complex III
Mitochondrial Proteins
Physiology
Electron Transport
Inbred C57BL Mouse
Genetic Promoter Regions
Gene expression
Cell Survival
Fusion reactions
Genes

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Citar esto

Salazar, Celia ; Elorza, Alvaro A. ; Cofre, Glenda ; Ruiz-Hincapie, Paula ; Shirihai, Orian ; Ruiz, Lina María. / The OXPHOS supercomplex assembly factor HIG2A responds to changes in energetic metabolism and cell cycle. En: Journal of Cellular Physiology. 2019.
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abstract = "HIG2A promotes cell survival under hypoxia and mediates the assembly of complex III and complex IV into respiratory chain supercomplexes. In the present study, we show that human HIGD2A and mouse Higd2a gene expressions are regulated by hypoxia, glucose, and the cell cycle-related transcription factor E2F1. The latter was found to bind the promoter region of HIGD2A. Differential expression of the HIGD2A gene was found in C57BL/6 mice in relation to tissue and age. Besides, the silencing of HIGD2A evidenced the modulation of mitochondrial dynamics proteins namely, OPA1 as a fusion protein increases, while FIS1, a fission protein, decreases. Besides, the mitochondrial membrane potential (ΔΨm) increased. The protein HIG2A is localized in the mitochondria and nucleus. Moreover, we observed that the HIG2A protein interacts with OPA1. Changes in oxygen concentration, glucose availability, and cell cycle regulate HIGD2A expression. Alterations in HIGD2A expression are associated with changes in mitochondrial physiology.",
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The OXPHOS supercomplex assembly factor HIG2A responds to changes in energetic metabolism and cell cycle. / Salazar, Celia; Elorza, Alvaro A.; Cofre, Glenda; Ruiz-Hincapie, Paula; Shirihai, Orian; Ruiz, Lina María.

En: Journal of Cellular Physiology, 01.01.2019.

Resultado de la investigación: Article

TY - JOUR

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AU - Salazar, Celia

AU - Elorza, Alvaro A.

AU - Cofre, Glenda

AU - Ruiz-Hincapie, Paula

AU - Shirihai, Orian

AU - Ruiz, Lina María

PY - 2019/1/1

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N2 - HIG2A promotes cell survival under hypoxia and mediates the assembly of complex III and complex IV into respiratory chain supercomplexes. In the present study, we show that human HIGD2A and mouse Higd2a gene expressions are regulated by hypoxia, glucose, and the cell cycle-related transcription factor E2F1. The latter was found to bind the promoter region of HIGD2A. Differential expression of the HIGD2A gene was found in C57BL/6 mice in relation to tissue and age. Besides, the silencing of HIGD2A evidenced the modulation of mitochondrial dynamics proteins namely, OPA1 as a fusion protein increases, while FIS1, a fission protein, decreases. Besides, the mitochondrial membrane potential (ΔΨm) increased. The protein HIG2A is localized in the mitochondria and nucleus. Moreover, we observed that the HIG2A protein interacts with OPA1. Changes in oxygen concentration, glucose availability, and cell cycle regulate HIGD2A expression. Alterations in HIGD2A expression are associated with changes in mitochondrial physiology.

AB - HIG2A promotes cell survival under hypoxia and mediates the assembly of complex III and complex IV into respiratory chain supercomplexes. In the present study, we show that human HIGD2A and mouse Higd2a gene expressions are regulated by hypoxia, glucose, and the cell cycle-related transcription factor E2F1. The latter was found to bind the promoter region of HIGD2A. Differential expression of the HIGD2A gene was found in C57BL/6 mice in relation to tissue and age. Besides, the silencing of HIGD2A evidenced the modulation of mitochondrial dynamics proteins namely, OPA1 as a fusion protein increases, while FIS1, a fission protein, decreases. Besides, the mitochondrial membrane potential (ΔΨm) increased. The protein HIG2A is localized in the mitochondria and nucleus. Moreover, we observed that the HIG2A protein interacts with OPA1. Changes in oxygen concentration, glucose availability, and cell cycle regulate HIGD2A expression. Alterations in HIGD2A expression are associated with changes in mitochondrial physiology.

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