The ompW (porin) gene mediates methyl viologen (paraquat) efflux in Salmonella enterica serovar Typhimurium

Fernando Gil, Francisco Ipinza, Juan Fuentes, Robinson Fumeron, José M. Villarreal, Alexis Aspée, Guido C. Mora, Claudio C. Vásquez, Claudia Saavedra

Resultado de la investigación: Contribución a una revistaArtículo

44 Citas (Scopus)

Resumen

Porins are channels that enable passive diffusion of hydrophilic solutes, nutrients and toxins through the outer bacterial membrane. This explains in part the ability of Gram-negative microorganisms to grow in several different environments, as well as their drug resistance. OmpD is an outer membrane channel that works with the inner membrane pump YddG to expel methyl viologen (MV) from Salmonella enterica serovar Typhimurium; this occurs independently of SmvA, also involved in MV resistance. On the other hand, ΔtolC strains show increased MV resistance when compared to wild-type cells, suggesting that there may be other porin(s) that could function with SmvA to pump MV out of S. typhimurium. A strong candidate is OmpW. Here we show that ΔompW strains of S. typhimurium are 2.5-fold more sensitive to MV than the wild-type strain. Transcriptional fusions replacing ompW by lacZ show that ompW is induced at least 2-fold in the presence of MV. This result was observed both at the mRNA and protein levels, suggesting that ompW participates in MV resistance. In addition, ΔsmvAΔompW strains are not fully complemented by smvA, suggesting that OmpW may function through an independent pathway different from that used by SmvA to move MV outside the cell.

Idioma originalInglés
Páginas (desde-hasta)529-536
Número de páginas8
PublicaciónResearch in Microbiology
Volumen158
N.º6
DOI
EstadoPublicada - 1 jul 2007

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Biología molecular

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