The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells

Bertsy Goić, Juanita Bustamante, Alvaro Miquel, Marco Alvarez, M. Ines Vera, Pablo D T Valenzuela, Luis O. Burzio

Resultado de la investigación: Article

16 Citas (Scopus)

Resumen

The infectious salmon anemia virus (ISAV), which belongs to the new genus Isavirus of the Orthomyxoviridae family, is an important pathogen of the salmon farming industry. Indirect immunofluorescence assays carried out with monoclonal antibodies specific for the nucleoprotein (NP) reveal differential staining of sub-cellular compartments in infected cells. Particularly interesting was the staining of the nucleolus, which showed co-localization with nucleolin in CHSE-214, EPC and SHK-1 cells infected with ISAV. These results were confirmed by co-immunoprecipitation studies showing an interaction between NP and nucleolin. In addition, in situ hybridization carried out with probes specific for each of the 8 RNA segments of ISAV showed that the genomic as well as the anti-genomic strands were also localized in the nucleolus. These results suggest a role of the nucleolus in the replication and/or in the packaging of the ISAV genome.

Idioma originalEnglish
Páginas (desde-hasta)55-63
Número de páginas9
PublicaciónVirology
Volumen379
N.º1
DOI
EstadoPublished - 15 sep 2008

Huella dactilar

Isavirus
Cell Nucleolus
Nucleoproteins
Viral RNA
Staining and Labeling
Salmon
Product Packaging
Indirect Fluorescent Antibody Technique
Orthomyxoviridae
Agriculture
Immunoprecipitation
In Situ Hybridization
Industry
Monoclonal Antibodies
Genome
RNA

ASJC Scopus subject areas

  • Virology

Citar esto

Goić, Bertsy ; Bustamante, Juanita ; Miquel, Alvaro ; Alvarez, Marco ; Vera, M. Ines ; Valenzuela, Pablo D T ; Burzio, Luis O. / The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells. En: Virology. 2008 ; Vol. 379, N.º 1. pp. 55-63.
@article{449cf9b5b01c4af8b9ea29a01b8e42a1,
title = "The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells",
abstract = "The infectious salmon anemia virus (ISAV), which belongs to the new genus Isavirus of the Orthomyxoviridae family, is an important pathogen of the salmon farming industry. Indirect immunofluorescence assays carried out with monoclonal antibodies specific for the nucleoprotein (NP) reveal differential staining of sub-cellular compartments in infected cells. Particularly interesting was the staining of the nucleolus, which showed co-localization with nucleolin in CHSE-214, EPC and SHK-1 cells infected with ISAV. These results were confirmed by co-immunoprecipitation studies showing an interaction between NP and nucleolin. In addition, in situ hybridization carried out with probes specific for each of the 8 RNA segments of ISAV showed that the genomic as well as the anti-genomic strands were also localized in the nucleolus. These results suggest a role of the nucleolus in the replication and/or in the packaging of the ISAV genome.",
keywords = "In situ hybridization, ISAV, Nucleoli, Nucleolin, Nucleoprotein",
author = "Bertsy Goić and Juanita Bustamante and Alvaro Miquel and Marco Alvarez and Vera, {M. Ines} and Valenzuela, {Pablo D T} and Burzio, {Luis O.}",
year = "2008",
month = "9",
day = "15",
doi = "10.1016/j.virol.2008.05.036",
language = "English",
volume = "379",
pages = "55--63",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells. / Goić, Bertsy; Bustamante, Juanita; Miquel, Alvaro; Alvarez, Marco; Vera, M. Ines; Valenzuela, Pablo D T; Burzio, Luis O.

En: Virology, Vol. 379, N.º 1, 15.09.2008, p. 55-63.

Resultado de la investigación: Article

TY - JOUR

T1 - The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells

AU - Goić, Bertsy

AU - Bustamante, Juanita

AU - Miquel, Alvaro

AU - Alvarez, Marco

AU - Vera, M. Ines

AU - Valenzuela, Pablo D T

AU - Burzio, Luis O.

PY - 2008/9/15

Y1 - 2008/9/15

N2 - The infectious salmon anemia virus (ISAV), which belongs to the new genus Isavirus of the Orthomyxoviridae family, is an important pathogen of the salmon farming industry. Indirect immunofluorescence assays carried out with monoclonal antibodies specific for the nucleoprotein (NP) reveal differential staining of sub-cellular compartments in infected cells. Particularly interesting was the staining of the nucleolus, which showed co-localization with nucleolin in CHSE-214, EPC and SHK-1 cells infected with ISAV. These results were confirmed by co-immunoprecipitation studies showing an interaction between NP and nucleolin. In addition, in situ hybridization carried out with probes specific for each of the 8 RNA segments of ISAV showed that the genomic as well as the anti-genomic strands were also localized in the nucleolus. These results suggest a role of the nucleolus in the replication and/or in the packaging of the ISAV genome.

AB - The infectious salmon anemia virus (ISAV), which belongs to the new genus Isavirus of the Orthomyxoviridae family, is an important pathogen of the salmon farming industry. Indirect immunofluorescence assays carried out with monoclonal antibodies specific for the nucleoprotein (NP) reveal differential staining of sub-cellular compartments in infected cells. Particularly interesting was the staining of the nucleolus, which showed co-localization with nucleolin in CHSE-214, EPC and SHK-1 cells infected with ISAV. These results were confirmed by co-immunoprecipitation studies showing an interaction between NP and nucleolin. In addition, in situ hybridization carried out with probes specific for each of the 8 RNA segments of ISAV showed that the genomic as well as the anti-genomic strands were also localized in the nucleolus. These results suggest a role of the nucleolus in the replication and/or in the packaging of the ISAV genome.

KW - In situ hybridization

KW - ISAV

KW - Nucleoli

KW - Nucleolin

KW - Nucleoprotein

UR - http://www.scopus.com/inward/record.url?scp=49049095668&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2008.05.036

DO - 10.1016/j.virol.2008.05.036

M3 - Article

C2 - 18632128

AN - SCOPUS:49049095668

VL - 379

SP - 55

EP - 63

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -