The major component of the rat sperm fibrous sheath is a phosphoprotein

Monica Brito, Jaime Figueroa, Enrique U. Maldonado, Juan C. Vera, Luis O. Burzio

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

34 Citas (Scopus)

Resumen

The fibrous sheath from rat epididymal sperm was isolated by sequential extraction, first with Triton X‐100 and dithiothreitol, and then with 6 M urea and dithiothreitol. The latter extraction procedure solubilized most of the sperm components, leaving the head and the fibrous sheath as the only intact structures. This material was purified by sucrose gradient centrifugation. Electron microscopy confirmed the purity of the isolated material and revealed the characteristic structural features of the fibrous sheath. Polyacrylamide gel electrophoresis (in the presence of sodium dodecyl sulfate) of the fibrillar material, showed a complex polypeptide composition. The polypeptides with molecular weights of 80,000, 24,000, and 11,500 accounted for about 65% of the total protein of the fibrous sheath. Peptide map analyses indicated that the components of molecular weights of 80,000 and 24,000 are unrelated to the polypeptides of similar size of the outer dense fibers. On the other hand, it appears that the fibrous sheath and the outer dense fibers share the polypeptide of 11,500 daltons. The component of 80,000 daltons contains on the average about 3 mol of phosphoserine per mol of polypeptide, indicating that the most abundant polypeptide of the fibrous sheath is a phosphoprotein.

Idioma originalInglés
Páginas (desde-hasta)205-217
Número de páginas13
PublicaciónGamete Research
Volumen22
N.º2
DOI
EstadoPublicada - feb. 1989
Publicado de forma externa

Áreas temáticas de ASJC Scopus

  • Genética
  • Biología del desarrollo

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