The low density lipoprotein receptor-related protein functions as an endocytic receptor for decorin

Enrique Brandan, Claudio Retamal, Claudio Cabello-Verrugio, María Paz Marzolo

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

47 Citas (Scopus)

Resumen

Decorin is a small leucine-rich proteoglycan that modulates the activity of transforming growth factor type β and other growth factors and thereby influences the processes of proliferation and differentiation in a wide array of physiological and pathological reactions. Hence, understanding the regulatory mechanisms of decorin activity has broad implications. Here we report that the extracellular levels of decorin are controlled by receptor-mediated catabolism, involving the low density lipoprotein receptor family member, low density lipoprotein receptor-related protein (LRP). We show that decorin is endocytosed and degraded by C2C12 myoblast cells and that both processes are blocked by suppressing LRP expression using short interfering RNA. The same occurs with CHO cells, but not with CHO cells genetically deficient in LRP. Finally, we show that LRP-null CHO cells, transfected to express mini-LRP polypeptides containing either the second or fourth LRP ligand-binding domains, carry out decorin endocytosis and lysosomal degradation. These findings point to LRP-mediated catabolism as a new control pathway for the biological activities of decorin, specifically for its ability to influence extracellular matrix signaling.

Idioma originalInglés
Páginas (desde-hasta)31562-31571
Número de páginas10
PublicaciónJournal of Biological Chemistry
Volumen281
N.º42
DOI
EstadoPublicada - 20 oct 2006

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Biología molecular
  • Biología celular

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