The hypoxia factor Hif-1α controls neural crest chemotaxis and epithelial to mesenchymal transition

Elias H. Barriga, Patrick H. Maxwell, Ariel E. Reyes, Roberto Mayor

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

83 Citas (Scopus)

Resumen

One of the most important mechanisms that promotes metastasis is the stabilization of Hif-1 (hypoxia-inducible transcription factor 1). We decided to test whether Hif-1α also was required for early embryonic development. We focused our attention on the development of the neural crest, a highly migratory embryonic cell population whose behavior has been likened to cancer metastasis. Inhibition of Hif-1α by antisense morpholinos in Xenopus laevis or zebrafish embryos led to complete inhibition of neural crest migration. We show that Hif-1α controls the expression of Twist, which in turn represses E-cadherin during epithelial to mesenchymal transition (EMT) of neural crest cells. Thus, Hif-1α allows cells to initiate migration by promoting the release of cell- cell adhesions. Additionally, Hif-1α controls chemotaxis toward the chemokine SDF-1 by regulating expression of its receptor Cxcr4. Our results point to Hif-1α as a novel and key regulator that integrates EMT and chemotaxis during migration of neural crest cells.

Idioma originalInglés
Páginas (desde-hasta)759-776
Número de páginas18
PublicaciónJournal of Cell Biology
Volumen201
N.º5
DOI
EstadoPublicada - may 2013

Áreas temáticas de ASJC Scopus

  • Biología celular

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