The HP1α-CAF1-SetDB1-containing complex provides H3K9me1 for Suv39-mediated K9me3 in pericentric heterochromatin

Alejandra Loyola, Hideaki Tagami, Tiziana Bonaldi, Danièle Roche, Jean Pierre Quivy, Axel Imhof, Yoshihiro Nakatani, Sharon Y.R. Dent, Geneviève Almouzni

Resultado de la investigación: Article

131 Citas (Scopus)

Resumen

Trimethylation of lysine 9 in histone H3 (H3K9me3) enrichment is a characteristic of pericentric heterochromatin. The hypothesis of a stepwise mechanism to establish and maintain this mark during DNA replication suggests that newly synthesized histone H3 goes through an intermediate methylation state to become a substrate for the histone methyltransferase Suppressor of variegation 39 (Suv39H1/H2). How this intermediate methylation state is achieved and how it is targeted to the correct place at the right time is not yet known. Here, we show that the histone H3K9 methyltransferase SetDB1 associates with the specific heterochromatin protein 1α (HP1α)-chromatin assembly factor 1 (CAF1) chaperone complex. This complex monomethylates K9 on non-nucleosomal histone H3. Therefore, the heterochromatic HP1α-CAF1-SetDB1 complex probably provides H3K9me1 for subsequent trimethylation by Suv39H1/H2 in pericentric regions. The connection of CAF1 with DNA replication, HP1α with heterochromatin formation and SetDB1 for H3K9me1 suggests a highly coordinated mechanism to ensure the propagation of H3K9me3 in pericentric heterochromatin during DNA replication.

Idioma originalEnglish
Páginas (desde-hasta)769-775
Número de páginas7
PublicaciónEMBO Reports
Volumen10
N.º7
DOI
EstadoPublished - 2009

Huella dactilar

Chromatin Assembly Factor-1
Heterochromatin
DNA Replication
Histones
Methylation
DNA
Lysine
Substrates
heterochromatin-specific nonhistone chromosomal protein HP-1
histone methyltransferase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Citar esto

Loyola, A., Tagami, H., Bonaldi, T., Roche, D., Quivy, J. P., Imhof, A., ... Almouzni, G. (2009). The HP1α-CAF1-SetDB1-containing complex provides H3K9me1 for Suv39-mediated K9me3 in pericentric heterochromatin. EMBO Reports, 10(7), 769-775. https://doi.org/10.1038/embor.2009.90
Loyola, Alejandra ; Tagami, Hideaki ; Bonaldi, Tiziana ; Roche, Danièle ; Quivy, Jean Pierre ; Imhof, Axel ; Nakatani, Yoshihiro ; Dent, Sharon Y.R. ; Almouzni, Geneviève. / The HP1α-CAF1-SetDB1-containing complex provides H3K9me1 for Suv39-mediated K9me3 in pericentric heterochromatin. En: EMBO Reports. 2009 ; Vol. 10, N.º 7. pp. 769-775.
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abstract = "Trimethylation of lysine 9 in histone H3 (H3K9me3) enrichment is a characteristic of pericentric heterochromatin. The hypothesis of a stepwise mechanism to establish and maintain this mark during DNA replication suggests that newly synthesized histone H3 goes through an intermediate methylation state to become a substrate for the histone methyltransferase Suppressor of variegation 39 (Suv39H1/H2). How this intermediate methylation state is achieved and how it is targeted to the correct place at the right time is not yet known. Here, we show that the histone H3K9 methyltransferase SetDB1 associates with the specific heterochromatin protein 1α (HP1α)-chromatin assembly factor 1 (CAF1) chaperone complex. This complex monomethylates K9 on non-nucleosomal histone H3. Therefore, the heterochromatic HP1α-CAF1-SetDB1 complex probably provides H3K9me1 for subsequent trimethylation by Suv39H1/H2 in pericentric regions. The connection of CAF1 with DNA replication, HP1α with heterochromatin formation and SetDB1 for H3K9me1 suggests a highly coordinated mechanism to ensure the propagation of H3K9me3 in pericentric heterochromatin during DNA replication.",
author = "Alejandra Loyola and Hideaki Tagami and Tiziana Bonaldi and Dani{\`e}le Roche and Quivy, {Jean Pierre} and Axel Imhof and Yoshihiro Nakatani and Dent, {Sharon Y.R.} and Genevi{\`e}ve Almouzni",
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Loyola, A, Tagami, H, Bonaldi, T, Roche, D, Quivy, JP, Imhof, A, Nakatani, Y, Dent, SYR & Almouzni, G 2009, 'The HP1α-CAF1-SetDB1-containing complex provides H3K9me1 for Suv39-mediated K9me3 in pericentric heterochromatin', EMBO Reports, vol. 10, n.º 7, pp. 769-775. https://doi.org/10.1038/embor.2009.90

The HP1α-CAF1-SetDB1-containing complex provides H3K9me1 for Suv39-mediated K9me3 in pericentric heterochromatin. / Loyola, Alejandra; Tagami, Hideaki; Bonaldi, Tiziana; Roche, Danièle; Quivy, Jean Pierre; Imhof, Axel; Nakatani, Yoshihiro; Dent, Sharon Y.R.; Almouzni, Geneviève.

En: EMBO Reports, Vol. 10, N.º 7, 2009, p. 769-775.

Resultado de la investigación: Article

TY - JOUR

T1 - The HP1α-CAF1-SetDB1-containing complex provides H3K9me1 for Suv39-mediated K9me3 in pericentric heterochromatin

AU - Loyola, Alejandra

AU - Tagami, Hideaki

AU - Bonaldi, Tiziana

AU - Roche, Danièle

AU - Quivy, Jean Pierre

AU - Imhof, Axel

AU - Nakatani, Yoshihiro

AU - Dent, Sharon Y.R.

AU - Almouzni, Geneviève

PY - 2009

Y1 - 2009

N2 - Trimethylation of lysine 9 in histone H3 (H3K9me3) enrichment is a characteristic of pericentric heterochromatin. The hypothesis of a stepwise mechanism to establish and maintain this mark during DNA replication suggests that newly synthesized histone H3 goes through an intermediate methylation state to become a substrate for the histone methyltransferase Suppressor of variegation 39 (Suv39H1/H2). How this intermediate methylation state is achieved and how it is targeted to the correct place at the right time is not yet known. Here, we show that the histone H3K9 methyltransferase SetDB1 associates with the specific heterochromatin protein 1α (HP1α)-chromatin assembly factor 1 (CAF1) chaperone complex. This complex monomethylates K9 on non-nucleosomal histone H3. Therefore, the heterochromatic HP1α-CAF1-SetDB1 complex probably provides H3K9me1 for subsequent trimethylation by Suv39H1/H2 in pericentric regions. The connection of CAF1 with DNA replication, HP1α with heterochromatin formation and SetDB1 for H3K9me1 suggests a highly coordinated mechanism to ensure the propagation of H3K9me3 in pericentric heterochromatin during DNA replication.

AB - Trimethylation of lysine 9 in histone H3 (H3K9me3) enrichment is a characteristic of pericentric heterochromatin. The hypothesis of a stepwise mechanism to establish and maintain this mark during DNA replication suggests that newly synthesized histone H3 goes through an intermediate methylation state to become a substrate for the histone methyltransferase Suppressor of variegation 39 (Suv39H1/H2). How this intermediate methylation state is achieved and how it is targeted to the correct place at the right time is not yet known. Here, we show that the histone H3K9 methyltransferase SetDB1 associates with the specific heterochromatin protein 1α (HP1α)-chromatin assembly factor 1 (CAF1) chaperone complex. This complex monomethylates K9 on non-nucleosomal histone H3. Therefore, the heterochromatic HP1α-CAF1-SetDB1 complex probably provides H3K9me1 for subsequent trimethylation by Suv39H1/H2 in pericentric regions. The connection of CAF1 with DNA replication, HP1α with heterochromatin formation and SetDB1 for H3K9me1 suggests a highly coordinated mechanism to ensure the propagation of H3K9me3 in pericentric heterochromatin during DNA replication.

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U2 - 10.1038/embor.2009.90

DO - 10.1038/embor.2009.90

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SP - 769

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