The Geobacillus stearothermophilus V iscS gene, encoding cysteine desulfurase, confers resistance to potassium tellurite in escherichia coli K-12

Juan C. Tantaleán, Manuel A. Araya, Claudia P. Saavedra, Derie E. Fuentes, José M. Pérez, Iván L. Calderón, Philip Youderian, Claudio C. Vásquez

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

39 Citas (Scopus)

Resumen

Many eubacteria are resistant to the toxic oxidizing agent potassium tellurite, and tellurite resistance involves diverse biochemical mechanisms. Expression of the iscS gene from Geobacillus stearothermophilus V, which is naturally resistant to tellurite, confers tellurite resistance in Escherichia coli K-12, which is naturally sensitive to tellurite. The G. stearothermophilus iscS gene encodes a cysteine desulfurase, A site-directed mutation in iscS that prevents binding of its pyridoxal phosphate cofactor abolishes both enzyme activity and its ability to confer tellurite resistance in E. coli. Expression of the G. stearothermophilus iscS gene confers tellurite resistance in tellurite-hypersensitive E. coli iscS and sodA sodB mutants (deficient in superoxide dismutase) and complements the auxotrophic requirement of an E. coli iscS mutant for thiamine but not for nicotinic acid, These and other results support the hypothesis that the reduction of tellurite generates superoxide anions and that the primary targets of superoxide damage in E. coli are enzymes with iron-sulfur clusters.

Idioma originalInglés
Páginas (desde-hasta)5831-5837
Número de páginas7
PublicaciónJournal of Bacteriology
Volumen185
N.º19
DOI
EstadoPublicada - oct 2003

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Biología molecular

Huella

Profundice en los temas de investigación de 'The Geobacillus stearothermophilus V iscS gene, encoding cysteine desulfurase, confers resistance to potassium tellurite in escherichia coli K-12'. En conjunto forman una huella única.

Citar esto