The genome sequence of the soft-rot fungus Penicillium purpurogenum reveals a high gene dosage for lignocellulolytic enzymes

Wladimir Mardones, Alex Di Genova, María Paz Cortés, Dante Travisany, Alejandro Maass, Jaime Eyzaguirre

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

The high lignocellulolytic activity displayed by the soft-rot fungus Penicillium purpurogenum has made it a target for the study of novel lignocellulolytic enzymes. We have obtained a reference genome of 36.2 Mb of non-redundant sequence (11,057 protein-coding genes). The 49 largest scaffolds cover 90% of the assembly, and Core Eukaryotic Genes Mapping Approach (CEGMA) analysis reveals that our assembly captures almost all protein-coding genes. RNA-seq was performed and 93.1% of the reads aligned to the assembled genome. These data, plus the independent sequencing of a set of genes of lignocellulose-degrading enzymes, validate the quality of the genome sequence. P. purpurogenum shows a higher number of proteins with CAZy motifs, transcription factors and transporters as compared to other sequenced Penicillia. These results demonstrate the great potential for lignocellulolytic activity of this fungus and the possible use of its enzymes in related industrial applications.

Idioma originalEnglish
Páginas (desde-hasta)59-69
Número de páginas11
PublicaciónMycology
Volumen9
N.º1
DOI
EstadoPublished - 2 ene 2018

Huella dactilar

soft-rot fungi
Talaromyces purpurogenus
gene dosage
Gene Dosage
Penicillium
Fungi
Genome
genome
Enzymes
enzymes
lignocellulose
Proteins
genes
proteins
Chromosome Mapping
industrial applications
chromosome mapping
transporters
Transcription Factors
transcription factors

ASJC Scopus subject areas

  • Microbiology
  • Plant Science
  • Infectious Diseases

Citar esto

Mardones, Wladimir ; Di Genova, Alex ; Cortés, María Paz ; Travisany, Dante ; Maass, Alejandro ; Eyzaguirre, Jaime. / The genome sequence of the soft-rot fungus Penicillium purpurogenum reveals a high gene dosage for lignocellulolytic enzymes. En: Mycology. 2018 ; Vol. 9, N.º 1. pp. 59-69.
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The genome sequence of the soft-rot fungus Penicillium purpurogenum reveals a high gene dosage for lignocellulolytic enzymes. / Mardones, Wladimir; Di Genova, Alex; Cortés, María Paz; Travisany, Dante; Maass, Alejandro; Eyzaguirre, Jaime.

En: Mycology, Vol. 9, N.º 1, 02.01.2018, p. 59-69.

Resultado de la investigación: Article

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AU - Travisany, Dante

AU - Maass, Alejandro

AU - Eyzaguirre, Jaime

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AB - The high lignocellulolytic activity displayed by the soft-rot fungus Penicillium purpurogenum has made it a target for the study of novel lignocellulolytic enzymes. We have obtained a reference genome of 36.2 Mb of non-redundant sequence (11,057 protein-coding genes). The 49 largest scaffolds cover 90% of the assembly, and Core Eukaryotic Genes Mapping Approach (CEGMA) analysis reveals that our assembly captures almost all protein-coding genes. RNA-seq was performed and 93.1% of the reads aligned to the assembled genome. These data, plus the independent sequencing of a set of genes of lignocellulose-degrading enzymes, validate the quality of the genome sequence. P. purpurogenum shows a higher number of proteins with CAZy motifs, transcription factors and transporters as compared to other sequenced Penicillia. These results demonstrate the great potential for lignocellulolytic activity of this fungus and the possible use of its enzymes in related industrial applications.

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