The function of pseudouridylic acid in transfer RNA. III. Inactivation of formylmethionine transfer RNA of E. coli by cyanoethylation with acrylonitrile

M. A Q Siddiqui, Manuel Krauskopf, James Ofengand

Resultado de la investigación: Article

17 Citas (Scopus)

Resumen

Cyanoethylation of formylmethionine tRNA at 60° resulted in a rapid pseudofirst order inactivation of acceptor activity (T 1 2, 28 min). Under the conditions chosen, only the Ψrd in the T-Ψ-C-G loop should have reacted. Absorbancy-temperature profiles of 94% inactivated tRNA revealed a marked decrease in ordered structure which is probably responsible for the loss of activity.

Idioma originalEnglish
Páginas (desde-hasta)156-164
Número de páginas9
PublicaciónBiochemical and Biophysical Research Communications
Volumen38
N.º1
DOI
EstadoPublished - 6 ene 1970

Huella dactilar

Acrylonitrile
Transfer RNA
Escherichia coli
Temperature
formylmethionine-tRNA
pseudouridylic acid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Citar esto

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The function of pseudouridylic acid in transfer RNA. III. Inactivation of formylmethionine transfer RNA of E. coli by cyanoethylation with acrylonitrile. / Siddiqui, M. A Q; Krauskopf, Manuel; Ofengand, James.

En: Biochemical and Biophysical Research Communications, Vol. 38, N.º 1, 06.01.1970, p. 156-164.

Resultado de la investigación: Article

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AU - Ofengand, James

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