The fish pathogen vibrio ordalii under iron deprivation produces the siderophore piscibactin

Pamela Ruiz, Miguel Balado, Juan Carlos Fuentes-Monteverde, Alicia E. Toranzo, Jaime Rodríguez, Carlos Jiménez, Ruben Avendaño-Herrera, Manuel L. Lemos

Resultado de la investigación: Article

Resumen

Vibrio ordalii is the causative agent of vibriosis, mainly in salmonid fishes, and its virulence mechanisms are still not completely understood. In previous works we demonstrated that V. ordalii possess several iron uptake mechanisms based on heme utilization and siderophore production. The aim of the present work was to confirm the production and utilization of piscibactin as a siderophore by V. ordalii. Using genetic analysis, identification by peptide mass fingerprinting (PMF) of iron-regulated membrane proteins and chemical identification by LC-HRMS, we were able to clearly demonstrate that V. ordalii produces piscibactin under iron limitation. The synthesis and transport of this siderophore is encoded by a chromosomal gene cluster homologous to another one described in V. anguillarum, which also encodes the synthesis of piscibactin. Using β-galactosidase assays we were able to show that two potential promoters regulated by iron control the transcription of this gene cluster in V. ordalii. Moreover, biosynthetic and transport proteins corresponding to piscibactin synthesis and uptake could be identified in membrane fractions of V. ordalii cells grown under iron limitation. The synthesis of piscibactin was previously reported in other fish pathogens like Photobacterium damselae subsp. piscicida and V. anguillarum, which highlights the importance of this siderophore as a key virulence factor in Vibrionaceae bacteria infecting poikilothermic animals.

Idioma originalEnglish
Número de artículo313
PublicaciónMicroorganisms
Volumen7
N.º9
DOI
EstadoPublished - sep 2019

Huella dactilar

Siderophores
Vibrio
Fishes
Iron
Multigene Family
Vibrionaceae
Photobacterium
Galactosidases
Peptide Mapping
Virulence Factors
Heme
Recombinant Proteins
Virulence
Carrier Proteins
Membrane Proteins
Bacteria
Membranes

ASJC Scopus subject areas

  • Microbiology
  • Virology
  • Microbiology (medical)

Citar esto

Ruiz, P., Balado, M., Fuentes-Monteverde, J. C., Toranzo, A. E., Rodríguez, J., Jiménez, C., ... Lemos, M. L. (2019). The fish pathogen vibrio ordalii under iron deprivation produces the siderophore piscibactin. Microorganisms, 7(9), [313]. https://doi.org/10.3390/microorganisms7090313
Ruiz, Pamela ; Balado, Miguel ; Fuentes-Monteverde, Juan Carlos ; Toranzo, Alicia E. ; Rodríguez, Jaime ; Jiménez, Carlos ; Avendaño-Herrera, Ruben ; Lemos, Manuel L. / The fish pathogen vibrio ordalii under iron deprivation produces the siderophore piscibactin. En: Microorganisms. 2019 ; Vol. 7, N.º 9.
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abstract = "Vibrio ordalii is the causative agent of vibriosis, mainly in salmonid fishes, and its virulence mechanisms are still not completely understood. In previous works we demonstrated that V. ordalii possess several iron uptake mechanisms based on heme utilization and siderophore production. The aim of the present work was to confirm the production and utilization of piscibactin as a siderophore by V. ordalii. Using genetic analysis, identification by peptide mass fingerprinting (PMF) of iron-regulated membrane proteins and chemical identification by LC-HRMS, we were able to clearly demonstrate that V. ordalii produces piscibactin under iron limitation. The synthesis and transport of this siderophore is encoded by a chromosomal gene cluster homologous to another one described in V. anguillarum, which also encodes the synthesis of piscibactin. Using β-galactosidase assays we were able to show that two potential promoters regulated by iron control the transcription of this gene cluster in V. ordalii. Moreover, biosynthetic and transport proteins corresponding to piscibactin synthesis and uptake could be identified in membrane fractions of V. ordalii cells grown under iron limitation. The synthesis of piscibactin was previously reported in other fish pathogens like Photobacterium damselae subsp. piscicida and V. anguillarum, which highlights the importance of this siderophore as a key virulence factor in Vibrionaceae bacteria infecting poikilothermic animals.",
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Ruiz, P, Balado, M, Fuentes-Monteverde, JC, Toranzo, AE, Rodríguez, J, Jiménez, C, Avendaño-Herrera, R & Lemos, ML 2019, 'The fish pathogen vibrio ordalii under iron deprivation produces the siderophore piscibactin', Microorganisms, vol. 7, n.º 9, 313. https://doi.org/10.3390/microorganisms7090313

The fish pathogen vibrio ordalii under iron deprivation produces the siderophore piscibactin. / Ruiz, Pamela; Balado, Miguel; Fuentes-Monteverde, Juan Carlos; Toranzo, Alicia E.; Rodríguez, Jaime; Jiménez, Carlos; Avendaño-Herrera, Ruben; Lemos, Manuel L.

En: Microorganisms, Vol. 7, N.º 9, 313, 09.2019.

Resultado de la investigación: Article

TY - JOUR

T1 - The fish pathogen vibrio ordalii under iron deprivation produces the siderophore piscibactin

AU - Ruiz, Pamela

AU - Balado, Miguel

AU - Fuentes-Monteverde, Juan Carlos

AU - Toranzo, Alicia E.

AU - Rodríguez, Jaime

AU - Jiménez, Carlos

AU - Avendaño-Herrera, Ruben

AU - Lemos, Manuel L.

PY - 2019/9

Y1 - 2019/9

N2 - Vibrio ordalii is the causative agent of vibriosis, mainly in salmonid fishes, and its virulence mechanisms are still not completely understood. In previous works we demonstrated that V. ordalii possess several iron uptake mechanisms based on heme utilization and siderophore production. The aim of the present work was to confirm the production and utilization of piscibactin as a siderophore by V. ordalii. Using genetic analysis, identification by peptide mass fingerprinting (PMF) of iron-regulated membrane proteins and chemical identification by LC-HRMS, we were able to clearly demonstrate that V. ordalii produces piscibactin under iron limitation. The synthesis and transport of this siderophore is encoded by a chromosomal gene cluster homologous to another one described in V. anguillarum, which also encodes the synthesis of piscibactin. Using β-galactosidase assays we were able to show that two potential promoters regulated by iron control the transcription of this gene cluster in V. ordalii. Moreover, biosynthetic and transport proteins corresponding to piscibactin synthesis and uptake could be identified in membrane fractions of V. ordalii cells grown under iron limitation. The synthesis of piscibactin was previously reported in other fish pathogens like Photobacterium damselae subsp. piscicida and V. anguillarum, which highlights the importance of this siderophore as a key virulence factor in Vibrionaceae bacteria infecting poikilothermic animals.

AB - Vibrio ordalii is the causative agent of vibriosis, mainly in salmonid fishes, and its virulence mechanisms are still not completely understood. In previous works we demonstrated that V. ordalii possess several iron uptake mechanisms based on heme utilization and siderophore production. The aim of the present work was to confirm the production and utilization of piscibactin as a siderophore by V. ordalii. Using genetic analysis, identification by peptide mass fingerprinting (PMF) of iron-regulated membrane proteins and chemical identification by LC-HRMS, we were able to clearly demonstrate that V. ordalii produces piscibactin under iron limitation. The synthesis and transport of this siderophore is encoded by a chromosomal gene cluster homologous to another one described in V. anguillarum, which also encodes the synthesis of piscibactin. Using β-galactosidase assays we were able to show that two potential promoters regulated by iron control the transcription of this gene cluster in V. ordalii. Moreover, biosynthetic and transport proteins corresponding to piscibactin synthesis and uptake could be identified in membrane fractions of V. ordalii cells grown under iron limitation. The synthesis of piscibactin was previously reported in other fish pathogens like Photobacterium damselae subsp. piscicida and V. anguillarum, which highlights the importance of this siderophore as a key virulence factor in Vibrionaceae bacteria infecting poikilothermic animals.

KW - Fish pathogens

KW - Iron uptake

KW - Piscibactin

KW - Siderophores

KW - Vanchrobactin

KW - Vibrio ordalii

UR - http://www.scopus.com/inward/record.url?scp=85074322285&partnerID=8YFLogxK

U2 - 10.3390/microorganisms7090313

DO - 10.3390/microorganisms7090313

M3 - Article

AN - SCOPUS:85074322285

VL - 7

JO - Microorganisms

JF - Microorganisms

SN - 2076-2607

IS - 9

M1 - 313

ER -

Ruiz P, Balado M, Fuentes-Monteverde JC, Toranzo AE, Rodríguez J, Jiménez C y otros. The fish pathogen vibrio ordalii under iron deprivation produces the siderophore piscibactin. Microorganisms. 2019 sep;7(9). 313. https://doi.org/10.3390/microorganisms7090313