The electric organ of Discopyge tschudii: Its innervated face and the biology of acetylcholinesterase

Bernardita Méndez, Jorge Garrido, Mafalda Maldonado, Fabián M. Jaksic, Nibaldo C. Inestrosa

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

14 Citas (Scopus)

Resumen

1. An ultrastructural, histochemical, and biochemical study of the electric organ of the South American Torpedinid ray, Discopyge tschudii, was carried out. 2. Fine structural cytochemical localization of acetylcholinesterase (AChE) indicated that most of the esterase was associated with the basal lamina. Electron microscopy indicated no marked differences in the electrocyte ultrastructure between Discopyge and Torpedo californica. 3. Discopyge electric organ possessed three molecular forms, two asymmetric forms (16 S and 13 S) and one globular hydrophobic form (6.5 S). The asymmetric 16 S AChE form was solubilized by heparin, a sulfated glycosaminoglycan, suggesting that heparin-like macromolecules are involved in the binding of the enzyme to the basal lamina. 4. Our results show that cell-free translated AChE peptides, synthesized using Discopyge electric organ poly (A+) RNA, correspond to a main band of 62,000 daltons which probably represents the catalytic subunit of the asymmetric AChE.

Idioma originalInglés
Páginas (desde-hasta)125-142
Número de páginas18
PublicaciónCellular and Molecular Neurobiology
Volumen4
N.º2
DOI
EstadoPublicada - jun 1984

Áreas temáticas de ASJC Scopus

  • Neurociencia (todo)
  • Genética
  • Bioquímica clínica
  • Biología celular
  • Neurociencia celular y molecular
  • Medicina (todo)

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