The copper-dependent ACE1 transcription factor activates the transcription of the mco1 gene from the basidiomycete Phanerochaete chrysosporium

Paulo Canessa, José Miguel Álvarez, Rubén Polanco, Paulina Bull, Rafael Vicuña

Resultado de la investigación: Article

18 Citas (Scopus)

Resumen

We have previously identified and functionally characterized the transcription factor ACE1 (Pc-ACE1) from Phanerochaete chrysosporium. In Saccharomyces cerevisiae, ACE1 activates the copper-dependent transcription of target genes through a DNA sequence element named ACE. However, the possible target gene(s) of Pc-ACE1 were unknowm. An in silico search led to the identification of putative ACE elements in the promoter region of mco1, one of the four clustered genes encoding multicopper oxidases (MCOs) in P. chrysosporium. Since copper exerts an effect at the transcriptional level in MCOs from several organisms, in this work we analysed the effect of copper on transcript levels of the clustered MCO genes from P. chrysosporium, with the exception of the transcriptionally inactive meo3. Copper supplementation of cultures produced an increment in transcripts from genes mco1 and mco2, but not from mco4. Electrophoretic mobility-shift assays revealed that Pc-ACE1 binds specifically to a probe containing one of the putative ACE elements found in the promoter of mco1. In addition, using a cell-free transcription system, we showed that in the presence of cuprous ion, Pc-ACE1 induces activation of the promoter of mco1, but not that of mco2.

Idioma originalEnglish
Páginas (desde-hasta)491-499
Número de páginas9
PublicaciónMicrobiology
Volumen154
N.º2
DOI
EstadoPublished - feb 2008

Huella dactilar

Phanerochaete
Basidiomycota
Copper
Transcription Factors
Oxidoreductases
Genes
Cell-Free System
Electrophoretic Mobility Shift Assay
Genetic Promoter Regions
Computer Simulation
Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Microbiology

Citar esto

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abstract = "We have previously identified and functionally characterized the transcription factor ACE1 (Pc-ACE1) from Phanerochaete chrysosporium. In Saccharomyces cerevisiae, ACE1 activates the copper-dependent transcription of target genes through a DNA sequence element named ACE. However, the possible target gene(s) of Pc-ACE1 were unknowm. An in silico search led to the identification of putative ACE elements in the promoter region of mco1, one of the four clustered genes encoding multicopper oxidases (MCOs) in P. chrysosporium. Since copper exerts an effect at the transcriptional level in MCOs from several organisms, in this work we analysed the effect of copper on transcript levels of the clustered MCO genes from P. chrysosporium, with the exception of the transcriptionally inactive meo3. Copper supplementation of cultures produced an increment in transcripts from genes mco1 and mco2, but not from mco4. Electrophoretic mobility-shift assays revealed that Pc-ACE1 binds specifically to a probe containing one of the putative ACE elements found in the promoter of mco1. In addition, using a cell-free transcription system, we showed that in the presence of cuprous ion, Pc-ACE1 induces activation of the promoter of mco1, but not that of mco2.",
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The copper-dependent ACE1 transcription factor activates the transcription of the mco1 gene from the basidiomycete Phanerochaete chrysosporium. / Canessa, Paulo; Álvarez, José Miguel; Polanco, Rubén; Bull, Paulina; Vicuña, Rafael.

En: Microbiology, Vol. 154, N.º 2, 02.2008, p. 491-499.

Resultado de la investigación: Article

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AU - Vicuña, Rafael

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