TY - JOUR
T1 - The combined effects of ocean acidification and warming on a habitat-forming shell-crushing predatory crab
AU - Manríquez, Patricio H.
AU - González, Claudio P.
AU - Seguel, Mylene
AU - Garcia-Huidobro, M. Roberto
AU - Lohrmann, Karin B.
AU - Domenici, Paolo
AU - Watson, Sue Ann
AU - Duarte, Cristián
AU - Brokordt, Katherina
N1 - Funding Information:
This study was financially supported by the Fondo Nacional de Desarrollo Científico y Tecnológico , FONDECYT grant No 1181609 to PHM and CD. During this study, PHM and PD were under the tenure of the grant “Climate driven Changes in the Habitat Suitability of Marine Organisms” (CLIMAR, ELAC2015/T01-0495) funded by the ERANet-LAC , the Network of the European Union , Latin America and the Caribbean Countries on Joint Innovation and Research Activities . During the writing of this manuscript CD and PHM were under the tenure of the FONDECYT grant No 1200794 . The authors also acknowledge the Millennium Nucleus Center for the Study of Multiple Drivers on Marine Socio-ecological Systems (MUSELS) funded by ICM MINECON and ANILLOS ACT172037 . MRG-H acknowledges grant ANID PAI77190031. We thank María Elisa Jara for assisting in the collection of species used in this study, María Isabel Díaz for assisting in the claw pinching force measurements and Rossana Rojas for assisting in the sarcomere measurements.
Publisher Copyright:
© 2020 Elsevier B.V.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - In mid rocky intertidal habitats the mussel Perumytilus purpurarus monopolizes the substratum to the detriment of many other species. However, the consumption of mussels by the shell-crushing crab Acanthocyclus hassleri creates within the mussel beds space and habitat for several other species. This crab uses its disproportionately large claw to crush its shelled prey and plays an important role in maintaining species biodiversity. This study evaluated the consequences of projected near-future ocean acidification (OA) and warming (OW) on traits of A. hassleri linked with their predatory performance. Individual A. hassleri were maintained for 10–16 weeks under contrasting pCO2 (~500 and 1400 μatm) and temperature (~15 and 20 °C) levels. We compared traits at the organismal (oxygen consumption rate, survival, calcification rate, feeding rates, crusher claw pinching strength, self-righting speed, sarcomere length of the crusher claw muscles) and cellular (nutritional status ATP provisioning capacity through citrate synthase activity, expression of HSP70) level. Survival, calcification rate and sarcomere length were not affected by OA and OW. However, OW increased significantly feeding and oxygen consumption. Pinching strength was reduced by OA; meanwhile self-righting was increased by OA and OW. At 20 °C, carbohydrate content was reduced significantly by OA. Regardless of temperature, a significant reduction in energy reserves in terms of protein content by OA was found. The ATP provisioning capacity was significantly affected by the interaction between temperature and pCO2 and was highest at 15 °C and present day pCO2 levels. The HSP70 levels of crabs exposed to OW were higher than in the control crabs. We conclude that OA and OW might affect the amount and size of prey consumed by this crab. Therefore, by reducing the crab feeding performance these stressors might pose limits on their role in generating microhabitat for other rocky intertidal species inhabiting within mussel beds.
AB - In mid rocky intertidal habitats the mussel Perumytilus purpurarus monopolizes the substratum to the detriment of many other species. However, the consumption of mussels by the shell-crushing crab Acanthocyclus hassleri creates within the mussel beds space and habitat for several other species. This crab uses its disproportionately large claw to crush its shelled prey and plays an important role in maintaining species biodiversity. This study evaluated the consequences of projected near-future ocean acidification (OA) and warming (OW) on traits of A. hassleri linked with their predatory performance. Individual A. hassleri were maintained for 10–16 weeks under contrasting pCO2 (~500 and 1400 μatm) and temperature (~15 and 20 °C) levels. We compared traits at the organismal (oxygen consumption rate, survival, calcification rate, feeding rates, crusher claw pinching strength, self-righting speed, sarcomere length of the crusher claw muscles) and cellular (nutritional status ATP provisioning capacity through citrate synthase activity, expression of HSP70) level. Survival, calcification rate and sarcomere length were not affected by OA and OW. However, OW increased significantly feeding and oxygen consumption. Pinching strength was reduced by OA; meanwhile self-righting was increased by OA and OW. At 20 °C, carbohydrate content was reduced significantly by OA. Regardless of temperature, a significant reduction in energy reserves in terms of protein content by OA was found. The ATP provisioning capacity was significantly affected by the interaction between temperature and pCO2 and was highest at 15 °C and present day pCO2 levels. The HSP70 levels of crabs exposed to OW were higher than in the control crabs. We conclude that OA and OW might affect the amount and size of prey consumed by this crab. Therefore, by reducing the crab feeding performance these stressors might pose limits on their role in generating microhabitat for other rocky intertidal species inhabiting within mussel beds.
KW - Acanthocyclus hassleri
KW - Global change biology
KW - Metabolic rate
KW - Nutritional status
KW - Pinching strength
KW - Self-righting
UR - http://www.scopus.com/inward/record.url?scp=85096367881&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2020.143587
DO - 10.1016/j.scitotenv.2020.143587
M3 - Article
AN - SCOPUS:85096367881
SN - 0048-9697
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 143587
ER -
