The Clostridium difficile exosporium cysteine (CdeC)-rich protein is required for exosporium morphogenesis and coat assembly

Jonathan Barra-Carrasco, Valeria Olguín-Araneda, Ángela Plaza-Garrido, Camila Miranda-Cárdenas, Glenda Cofré-Araneda, Marjorie Pizarro-Guajardo, Mahfuzur R. Sarker, Daniel Paredes-Sabja

Resultado de la investigación: Article

41 Citas (Scopus)

Resumen

Clostridium difficile is an important nosocomial pathogen that has become a major cause of antibiotic-associated diarrhea. There is a general consensus that C. difficile spores play an important role in C. difficile pathogenesis, contributing to infection, persistence, and transmission. Evidence has demonstrated that C. difficile spores have an outermost layer, termed the exosporium, that plays some role in adherence to intestinal epithelial cells. Recently, the protein encoded by CD1067 was shown to be present in trypsin-exosporium extracts of C. difficile 630 spores. In this study, we renamed the CD1067 protein Clostridium difficile exosporium cysteine-rich protein (CdeC) and characterized its role in the structure and properties of C. difficile spores. CdeC is expressed under sporulation conditions and localizes to the C. difficile spore. Through the construction of an ΔcdeC isogenic knockout mutant derivative of C. difficile strain R20291, we demonstrated that (i) the distinctive nap layer is largely missing in ΔcdeC spores; (ii) CdeC is localized in the exosporium-like layer and is accessible to IgGs; (iii) ΔcdeC spores were more sensitive to lysozyme, ethanol, and heat treatment than wild-type spores; and (iv) despite the almost complete absence of the exosporium layer, ΔcdeC spores adhered at higher levels than wild-type spores to intestinal epithelium cell lines (i.e., HT-29 and Caco-2 cells). Collectively, these results indicate that CdeC is essential for exosporium morphogenesis and the correct assembly of the spore coat of C. difficile.

Idioma originalEnglish
Páginas (desde-hasta)3863-3875
Número de páginas13
PublicaciónJournal of Bacteriology
Volumen195
N.º17
DOI
EstadoPublished - 2013

Huella dactilar

Clostridium difficile
Morphogenesis
Cysteine
Spores
Proteins
Infectious Disease Transmission
Caco-2 Cells
Intestinal Mucosa
Muramidase
Trypsin
Diarrhea
Ethanol
Hot Temperature

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Citar esto

Barra-Carrasco, J., Olguín-Araneda, V., Plaza-Garrido, Á., Miranda-Cárdenas, C., Cofré-Araneda, G., Pizarro-Guajardo, M., ... Paredes-Sabja, D. (2013). The Clostridium difficile exosporium cysteine (CdeC)-rich protein is required for exosporium morphogenesis and coat assembly. Journal of Bacteriology, 195(17), 3863-3875. https://doi.org/10.1128/JB.00369-13
Barra-Carrasco, Jonathan ; Olguín-Araneda, Valeria ; Plaza-Garrido, Ángela ; Miranda-Cárdenas, Camila ; Cofré-Araneda, Glenda ; Pizarro-Guajardo, Marjorie ; Sarker, Mahfuzur R. ; Paredes-Sabja, Daniel. / The Clostridium difficile exosporium cysteine (CdeC)-rich protein is required for exosporium morphogenesis and coat assembly. En: Journal of Bacteriology. 2013 ; Vol. 195, N.º 17. pp. 3863-3875.
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title = "The Clostridium difficile exosporium cysteine (CdeC)-rich protein is required for exosporium morphogenesis and coat assembly",
abstract = "Clostridium difficile is an important nosocomial pathogen that has become a major cause of antibiotic-associated diarrhea. There is a general consensus that C. difficile spores play an important role in C. difficile pathogenesis, contributing to infection, persistence, and transmission. Evidence has demonstrated that C. difficile spores have an outermost layer, termed the exosporium, that plays some role in adherence to intestinal epithelial cells. Recently, the protein encoded by CD1067 was shown to be present in trypsin-exosporium extracts of C. difficile 630 spores. In this study, we renamed the CD1067 protein Clostridium difficile exosporium cysteine-rich protein (CdeC) and characterized its role in the structure and properties of C. difficile spores. CdeC is expressed under sporulation conditions and localizes to the C. difficile spore. Through the construction of an ΔcdeC isogenic knockout mutant derivative of C. difficile strain R20291, we demonstrated that (i) the distinctive nap layer is largely missing in ΔcdeC spores; (ii) CdeC is localized in the exosporium-like layer and is accessible to IgGs; (iii) ΔcdeC spores were more sensitive to lysozyme, ethanol, and heat treatment than wild-type spores; and (iv) despite the almost complete absence of the exosporium layer, ΔcdeC spores adhered at higher levels than wild-type spores to intestinal epithelium cell lines (i.e., HT-29 and Caco-2 cells). Collectively, these results indicate that CdeC is essential for exosporium morphogenesis and the correct assembly of the spore coat of C. difficile.",
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Barra-Carrasco, J, Olguín-Araneda, V, Plaza-Garrido, Á, Miranda-Cárdenas, C, Cofré-Araneda, G, Pizarro-Guajardo, M, Sarker, MR & Paredes-Sabja, D 2013, 'The Clostridium difficile exosporium cysteine (CdeC)-rich protein is required for exosporium morphogenesis and coat assembly', Journal of Bacteriology, vol. 195, n.º 17, pp. 3863-3875. https://doi.org/10.1128/JB.00369-13

The Clostridium difficile exosporium cysteine (CdeC)-rich protein is required for exosporium morphogenesis and coat assembly. / Barra-Carrasco, Jonathan; Olguín-Araneda, Valeria; Plaza-Garrido, Ángela; Miranda-Cárdenas, Camila; Cofré-Araneda, Glenda; Pizarro-Guajardo, Marjorie; Sarker, Mahfuzur R.; Paredes-Sabja, Daniel.

En: Journal of Bacteriology, Vol. 195, N.º 17, 2013, p. 3863-3875.

Resultado de la investigación: Article

TY - JOUR

T1 - The Clostridium difficile exosporium cysteine (CdeC)-rich protein is required for exosporium morphogenesis and coat assembly

AU - Barra-Carrasco, Jonathan

AU - Olguín-Araneda, Valeria

AU - Plaza-Garrido, Ángela

AU - Miranda-Cárdenas, Camila

AU - Cofré-Araneda, Glenda

AU - Pizarro-Guajardo, Marjorie

AU - Sarker, Mahfuzur R.

AU - Paredes-Sabja, Daniel

PY - 2013

Y1 - 2013

N2 - Clostridium difficile is an important nosocomial pathogen that has become a major cause of antibiotic-associated diarrhea. There is a general consensus that C. difficile spores play an important role in C. difficile pathogenesis, contributing to infection, persistence, and transmission. Evidence has demonstrated that C. difficile spores have an outermost layer, termed the exosporium, that plays some role in adherence to intestinal epithelial cells. Recently, the protein encoded by CD1067 was shown to be present in trypsin-exosporium extracts of C. difficile 630 spores. In this study, we renamed the CD1067 protein Clostridium difficile exosporium cysteine-rich protein (CdeC) and characterized its role in the structure and properties of C. difficile spores. CdeC is expressed under sporulation conditions and localizes to the C. difficile spore. Through the construction of an ΔcdeC isogenic knockout mutant derivative of C. difficile strain R20291, we demonstrated that (i) the distinctive nap layer is largely missing in ΔcdeC spores; (ii) CdeC is localized in the exosporium-like layer and is accessible to IgGs; (iii) ΔcdeC spores were more sensitive to lysozyme, ethanol, and heat treatment than wild-type spores; and (iv) despite the almost complete absence of the exosporium layer, ΔcdeC spores adhered at higher levels than wild-type spores to intestinal epithelium cell lines (i.e., HT-29 and Caco-2 cells). Collectively, these results indicate that CdeC is essential for exosporium morphogenesis and the correct assembly of the spore coat of C. difficile.

AB - Clostridium difficile is an important nosocomial pathogen that has become a major cause of antibiotic-associated diarrhea. There is a general consensus that C. difficile spores play an important role in C. difficile pathogenesis, contributing to infection, persistence, and transmission. Evidence has demonstrated that C. difficile spores have an outermost layer, termed the exosporium, that plays some role in adherence to intestinal epithelial cells. Recently, the protein encoded by CD1067 was shown to be present in trypsin-exosporium extracts of C. difficile 630 spores. In this study, we renamed the CD1067 protein Clostridium difficile exosporium cysteine-rich protein (CdeC) and characterized its role in the structure and properties of C. difficile spores. CdeC is expressed under sporulation conditions and localizes to the C. difficile spore. Through the construction of an ΔcdeC isogenic knockout mutant derivative of C. difficile strain R20291, we demonstrated that (i) the distinctive nap layer is largely missing in ΔcdeC spores; (ii) CdeC is localized in the exosporium-like layer and is accessible to IgGs; (iii) ΔcdeC spores were more sensitive to lysozyme, ethanol, and heat treatment than wild-type spores; and (iv) despite the almost complete absence of the exosporium layer, ΔcdeC spores adhered at higher levels than wild-type spores to intestinal epithelium cell lines (i.e., HT-29 and Caco-2 cells). Collectively, these results indicate that CdeC is essential for exosporium morphogenesis and the correct assembly of the spore coat of C. difficile.

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Barra-Carrasco J, Olguín-Araneda V, Plaza-Garrido Á, Miranda-Cárdenas C, Cofré-Araneda G, Pizarro-Guajardo M y otros. The Clostridium difficile exosporium cysteine (CdeC)-rich protein is required for exosporium morphogenesis and coat assembly. Journal of Bacteriology. 2013;195(17):3863-3875. https://doi.org/10.1128/JB.00369-13