The classic receptor for 1α,25-dihydroxy vitamin D3 is required for non-genomic actions of 1α,25-dihydroxy vitamin D3 in osteosarcoma cells

Soraya Bravo, Roberto Paredes, Pamela Izaurieta, Jane B. Lian, Janet L. Stein, Gary S. Stein, Maria Victoria Hinrichs, Juan Olate, Luis G. Aguayo, Martin Montecino

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

14 Citas (Scopus)

Resumen

1α,25-dihydroxy vitamin D3 has a major role in the regulation of the bone metabolism as it promotes the expression of key bone-related proteins in osteoblastic cells. In recent years it has become increasingly evident that in addition to its well-established genomic actions, 1α,25-dihydroxy vitamin D3 induces non-genomic responses by acting through a specific plasma membrane-associated receptor. Results from several groups suggest that the classical nuclear 1α,25-dihydroxy vitamin D3 receptor (VDR) is also responsible for these non-genomic actions of 1α,25-dihydroxy vitamin D3. Here, we have used siRNA to suppress the expression of VDR in osteoblastic cells and assessed the role of VDR in the non-genomic response to 1α,25-dihydroxy vitamin D3. We report that expression of the classic VDR in osteoblasts is required to generate a rapid 1α,25-dihydroxy vitamin D3-mediated increase in the intracellular Ca 2+ concentration, a hallmark of the non-genomic actions of 1α,25-dihydroxy vitamin D3 in these cells.

Idioma originalInglés
Páginas (desde-hasta)995-1000
Número de páginas6
PublicaciónJournal of Cellular Biochemistry
Volumen99
N.º4
DOI
EstadoPublicada - 1 nov 2006

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Biología celular
  • Biología molecular

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