The chaperone ClpC participates in sporulation, motility, biofilm, and toxin production of Clostridioides difficile

Camila Queraltó; Constanza Ortega; Fernando Díaz-Yáñez; Osvaldo Inostroza; Giovanni Espinoza; Ricardo Álvarez; Ruth González; Francisco Parra; Daniel Paredes-Sabja; Lillian G. Acuña; Iván L. Calderón; Juan A. Fuentes; Fernando Gil

doi:10.1016/j.jgar.2023.05.004

The chaperone ClpC participates in sporulation, motility, biofilm, and toxin production of Clostridioides difficile

Camila Queraltó, Constanza Ortega, Fernando Díaz-Yáñez, Osvaldo Inostroza, Giovanni Espinoza, Ricardo Álvarez, Ruth González, Francisco Parra, Daniel Paredes-Sabja, Lillian G. Acuña, Iván L. Calderón, Juan A. Fuentes, Fernando Gil

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Resumen

Objectives: Clostridioides difficile is a nosocomial pathogen that is associated with the use of antibiotics. One of the most worrying aspects of C. difficile infection is its ability to resist antimicrobial therapies, owing to spore formation. In several bacterial pathogens, proteases of the Clp family participate in phenotypes associated with persistence and virulence. This suggests that these proteins could be involved in virulence-related traits. In this study, we analysed the role of ClpC chaperone-protease of C. difficile in virulence-related traits by comparing the phenotypes of wild-type and mutant strains lacking the clpC gene (ΔclpC). Methods: We performed biofilm, motility, spore formation, and cytotoxicity assays. Results: Our results show significant differences between the wild-type and ΔclpC strains in all analysed parameters. Conclusions: Based on these findings, we conclude that clpC plays a role in the virulence properties of C. difficile.

Idioma original	Inglés
Páginas (desde-hasta)	328-336
Número de páginas	9
Publicación	Journal of Global Antimicrobial Resistance
Volumen	33
DOI	https://doi.org/10.1016/j.jgar.2023.05.004
Estado	Publicada - jun. 2023

Áreas temáticas de ASJC Scopus

Microbiología
Inmulogía y alergología
Inmunología
Microbiología (médica)

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10.1016/j.jgar.2023.05.004

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Queraltó, C., Ortega, C., Díaz-Yáñez, F., Inostroza, O., Espinoza, G., Álvarez, R., González, R., Parra, F., Paredes-Sabja, D., Acuña, L. G., Calderón, I. L., Fuentes, J. A., & Gil, F. (2023). The chaperone ClpC participates in sporulation, motility, biofilm, and toxin production of Clostridioides difficile. Journal of Global Antimicrobial Resistance, 33, 328-336. https://doi.org/10.1016/j.jgar.2023.05.004

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title = "The chaperone ClpC participates in sporulation, motility, biofilm, and toxin production of Clostridioides difficile",

abstract = "Objectives: Clostridioides difficile is a nosocomial pathogen that is associated with the use of antibiotics. One of the most worrying aspects of C. difficile infection is its ability to resist antimicrobial therapies, owing to spore formation. In several bacterial pathogens, proteases of the Clp family participate in phenotypes associated with persistence and virulence. This suggests that these proteins could be involved in virulence-related traits. In this study, we analysed the role of ClpC chaperone-protease of C. difficile in virulence-related traits by comparing the phenotypes of wild-type and mutant strains lacking the clpC gene (ΔclpC). Methods: We performed biofilm, motility, spore formation, and cytotoxicity assays. Results: Our results show significant differences between the wild-type and ΔclpC strains in all analysed parameters. Conclusions: Based on these findings, we conclude that clpC plays a role in the virulence properties of C. difficile.",

keywords = "Clostridioides difficile, ClpC chaperone, virulence traits",

author = "Camila Queralt{\'o} and Constanza Ortega and Fernando D{\'i}az-Y{\'a}{\~n}ez and Osvaldo Inostroza and Giovanni Espinoza and Ricardo {\'A}lvarez and Ruth Gonz{\'a}lez and Francisco Parra and Daniel Paredes-Sabja and Acu{\~n}a, {Lillian G.} and Calder{\'o}n, {Iv{\'a}n L.} and Fuentes, {Juan A.} and Fernando Gil",

note = "Publisher Copyright: {\textcopyright} 2023 The Author(s)",

year = "2023",

month = jun,

doi = "10.1016/j.jgar.2023.05.004",

language = "English",

volume = "33",

pages = "328--336",

journal = "Journal of Global Antimicrobial Resistance",

issn = "2213-7165",

publisher = "Elsevier BV",

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Queraltó, C, Ortega, C, Díaz-Yáñez, F, Inostroza, O, Espinoza, G, Álvarez, R, González, R, Parra, F, Paredes-Sabja, D, Acuña, LG, Calderón, IL , Fuentes, JA & Gil, F 2023, 'The chaperone ClpC participates in sporulation, motility, biofilm, and toxin production of Clostridioides difficile', Journal of Global Antimicrobial Resistance, vol. 33, pp. 328-336. https://doi.org/10.1016/j.jgar.2023.05.004

TY - JOUR

T1 - The chaperone ClpC participates in sporulation, motility, biofilm, and toxin production of Clostridioides difficile

AU - Queraltó, Camila

AU - Ortega, Constanza

AU - Díaz-Yáñez, Fernando

AU - Inostroza, Osvaldo

AU - Espinoza, Giovanni

AU - Álvarez, Ricardo

AU - González, Ruth

AU - Parra, Francisco

AU - Paredes-Sabja, Daniel

AU - Acuña, Lillian G.

AU - Calderón, Iván L.

AU - Fuentes, Juan A.

AU - Gil, Fernando

PY - 2023/6

Y1 - 2023/6

N2 - Objectives: Clostridioides difficile is a nosocomial pathogen that is associated with the use of antibiotics. One of the most worrying aspects of C. difficile infection is its ability to resist antimicrobial therapies, owing to spore formation. In several bacterial pathogens, proteases of the Clp family participate in phenotypes associated with persistence and virulence. This suggests that these proteins could be involved in virulence-related traits. In this study, we analysed the role of ClpC chaperone-protease of C. difficile in virulence-related traits by comparing the phenotypes of wild-type and mutant strains lacking the clpC gene (ΔclpC). Methods: We performed biofilm, motility, spore formation, and cytotoxicity assays. Results: Our results show significant differences between the wild-type and ΔclpC strains in all analysed parameters. Conclusions: Based on these findings, we conclude that clpC plays a role in the virulence properties of C. difficile.

AB - Objectives: Clostridioides difficile is a nosocomial pathogen that is associated with the use of antibiotics. One of the most worrying aspects of C. difficile infection is its ability to resist antimicrobial therapies, owing to spore formation. In several bacterial pathogens, proteases of the Clp family participate in phenotypes associated with persistence and virulence. This suggests that these proteins could be involved in virulence-related traits. In this study, we analysed the role of ClpC chaperone-protease of C. difficile in virulence-related traits by comparing the phenotypes of wild-type and mutant strains lacking the clpC gene (ΔclpC). Methods: We performed biofilm, motility, spore formation, and cytotoxicity assays. Results: Our results show significant differences between the wild-type and ΔclpC strains in all analysed parameters. Conclusions: Based on these findings, we conclude that clpC plays a role in the virulence properties of C. difficile.

KW - Clostridioides difficile

KW - ClpC chaperone

KW - virulence traits

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U2 - 10.1016/j.jgar.2023.05.004

DO - 10.1016/j.jgar.2023.05.004

M3 - Article

C2 - 37211213

AN - SCOPUS:85161279869

SN - 2213-7165

VL - 33

SP - 328

EP - 336

JO - Journal of Global Antimicrobial Resistance

JF - Journal of Global Antimicrobial Resistance

ER -

The chaperone ClpC participates in sporulation, motility, biofilm, and toxin production of Clostridioides difficile

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