The cellular prion protein prevents copper-induced inhibition of P2X 4 receptors

Ramón A. Lorca, Lorena Varela-Nallar, Nibaldo C. Inestrosa, J. Pablo Huidobro-Toro

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

3 Citas (Scopus)

Resumen

Although the physiological function of the cellular prion protein (PrP C) remains unknown, several evidences support the notion of its role in copper homeostasis. PrP C binds Cu 2+ through a domain composed by four to five repeats of eight amino acids. Previously, we have shown that the perfusion of this domain prevents and reverses the inhibition by Cu 2+ of the adenosine triphosphate (ATP)-evoked currents in the P2X 4 receptor subtype, highlighting a modulatory role for PrP C in synaptic transmission through regulation of Cu 2+ levels. Here, we study the effect of full-length PrP C in Cu 2+ inhibition of P2X 4 receptor when both are coexpressed. PrP C expression does not significantly change the ATP concentration-response curve in oocytes expressing P2X 4 receptors. However, the presence of PrP C reduces the inhibition by Cu 2+ of the ATP-elicited currents in these oocytes, confirming our previous observations with the Cu 2+ binding domain. Thus, our observations suggest a role for PrP C in modulating synaptic activity through binding of extracellular Cu 2+.

Idioma originalInglés
Número de artículo706576
PublicaciónInternational Journal of Alzheimer's Disease
DOI
EstadoPublicada - 2011

Áreas temáticas de ASJC Scopus

  • Estudio del envejecimiento
  • Neurología
  • Neurología clínica
  • Neurociencia cognitiva
  • Neurociencia celular y molecular
  • Psicobiología

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