The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

Carsten Rautengarten; Berit Ebert; Lifeng Liu; Solomon Stonebloom; Andreia M. Smith-Moritz; Markus Pauly; Ariel Orellana; Henrik Vibe Scheller; Joshua L. Heazlewood

doi:10.1038/ncomms12119

The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

Carsten Rautengarten, Berit Ebert, Lifeng Liu, Solomon Stonebloom, Andreia M. Smith-Moritz, Markus Pauly, Ariel Orellana, Henrik Vibe Scheller, Joshua L. Heazlewood

Facultad de Ciencias de la Vida

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

53 Citas (Scopus)

Resumen

Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.

Idioma original	Inglés
Número de artículo	12119
Publicación	Nature Communications
Volumen	7
DOI	https://doi.org/10.1038/ncomms12119
Estado	Publicada - 6 jul. 2016

Áreas temáticas de ASJC Scopus

Química General
Bioquímica, Genética y Biología Molecular General
Física y Astronomía General

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title = "The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development",

abstract = "Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.",

author = "Carsten Rautengarten and Berit Ebert and Lifeng Liu and Solomon Stonebloom and Smith-Moritz, {Andreia M.} and Markus Pauly and Ariel Orellana and Scheller, {Henrik Vibe} and Heazlewood, {Joshua L.}",

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AU - Rautengarten, Carsten

AU - Ebert, Berit

AU - Liu, Lifeng

AU - Stonebloom, Solomon

AU - Smith-Moritz, Andreia M.

AU - Pauly, Markus

AU - Orellana, Ariel

AU - Scheller, Henrik Vibe

AU - Heazlewood, Joshua L.

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AB - Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.

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The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

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