Temporary immersion systems for the mass propagation of sweet cherry cultivars and cherry rootstocks: development of a micropropagation procedure and effect of culture conditions on plant quality

Sebastián Godoy, Eduardo Tapia, Paula Seit, Diego Andrade, Evelyn Sánchez, Paola Andrade, Andréa Miyasaka Almeida, Humberto Prieto

Resultado de la investigación: Article

5 Citas (Scopus)

Resumen

Sweet cherry (Prunus avium L.) varieties and cherry rootstocks are an important part of the fruit industry, and difficulties associated with mass propagation provide an opportunity for the use of temporary immersion systems (TIS). We show the establishment of culture procedures for four genotypes: the rootstocks Maxma-14 and Colt and the varieties ‘Van’ and ‘Rainier.’ The starting explants were internodal segments from seedlings kept in solid propagation medium (PM) (Driver-Kuniyuki Walnut (DKW) base supplemented with indole butyric acid, benzyl amino purine; ascorbic acid, myo-inositol, and agar). Segments were cultured under TIS for 14 d and led to whole plant generation after 30 d of culturing in solid rooting media, which depended on whether they are varieties or rootstocks. A 15-d acclimatization phase led to establishment in greenhouse. The efficiency of TIS was specifically analyzed for the two best PM-derivative media and compared to cultures using solid medium. A number of shoots (Px), biomass (Qx), and sucrose consumption (SC) were evaluated for these purposes. The results showed that Maxma-14, Colt, and ‘Van’ TIS cultures had improved performance in comparison to solid cultures, whereas ‘Rainier’ showed no differences. The number of immersions influenced all of the productive parameters (Px, Qx, and SC), whereas genotype affected Px, and the time of immersion influenced SC. The best Qx and Px values were obtained with the rootstocks Maxma-14 and Colt, as well as the variety Van; these showed no hyperhydration. Physiological studies show that 14-d TIS-produced shoots represented an intermediate stage between solid-derived and adult plants, although the photosynthetic efficiencies of these materials revealed a lack of autotrophic ability at this point.

Idioma originalEnglish
Páginas (desde-hasta)1-11
Número de páginas11
PublicaciónIn Vitro Cellular and Developmental Biology - Plant
Volumen53
N.º5
DOI
EstadoAccepted/In press - 21 sep 2017

Huella dactilar

Prunus avium
Immersion
micropropagation
rootstocks
colts
cultivars
sucrose
Sucrose
hyperhydricity
shoots
genotype
walnuts
Genotype
purines
myo-inositol
mature plants
indole butyric acid
Juglans
rooting
Butyric Acid

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science
  • Developmental Biology
  • Cell Biology

Citar esto

Godoy, Sebastián ; Tapia, Eduardo ; Seit, Paula ; Andrade, Diego ; Sánchez, Evelyn ; Andrade, Paola ; Almeida, Andréa Miyasaka ; Prieto, Humberto. / Temporary immersion systems for the mass propagation of sweet cherry cultivars and cherry rootstocks : development of a micropropagation procedure and effect of culture conditions on plant quality. En: In Vitro Cellular and Developmental Biology - Plant. 2017 ; Vol. 53, N.º 5. pp. 1-11.
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title = "Temporary immersion systems for the mass propagation of sweet cherry cultivars and cherry rootstocks: development of a micropropagation procedure and effect of culture conditions on plant quality",
abstract = "Sweet cherry (Prunus avium L.) varieties and cherry rootstocks are an important part of the fruit industry, and difficulties associated with mass propagation provide an opportunity for the use of temporary immersion systems (TIS). We show the establishment of culture procedures for four genotypes: the rootstocks Maxma-14 and Colt and the varieties ‘Van’ and ‘Rainier.’ The starting explants were internodal segments from seedlings kept in solid propagation medium (PM) (Driver-Kuniyuki Walnut (DKW) base supplemented with indole butyric acid, benzyl amino purine; ascorbic acid, myo-inositol, and agar). Segments were cultured under TIS for 14 d and led to whole plant generation after 30 d of culturing in solid rooting media, which depended on whether they are varieties or rootstocks. A 15-d acclimatization phase led to establishment in greenhouse. The efficiency of TIS was specifically analyzed for the two best PM-derivative media and compared to cultures using solid medium. A number of shoots (Px), biomass (Qx), and sucrose consumption (SC) were evaluated for these purposes. The results showed that Maxma-14, Colt, and ‘Van’ TIS cultures had improved performance in comparison to solid cultures, whereas ‘Rainier’ showed no differences. The number of immersions influenced all of the productive parameters (Px, Qx, and SC), whereas genotype affected Px, and the time of immersion influenced SC. The best Qx and Px values were obtained with the rootstocks Maxma-14 and Colt, as well as the variety Van; these showed no hyperhydration. Physiological studies show that 14-d TIS-produced shoots represented an intermediate stage between solid-derived and adult plants, although the photosynthetic efficiencies of these materials revealed a lack of autotrophic ability at this point.",
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Temporary immersion systems for the mass propagation of sweet cherry cultivars and cherry rootstocks : development of a micropropagation procedure and effect of culture conditions on plant quality. / Godoy, Sebastián; Tapia, Eduardo; Seit, Paula; Andrade, Diego; Sánchez, Evelyn; Andrade, Paola; Almeida, Andréa Miyasaka; Prieto, Humberto.

En: In Vitro Cellular and Developmental Biology - Plant, Vol. 53, N.º 5, 21.09.2017, p. 1-11.

Resultado de la investigación: Article

TY - JOUR

T1 - Temporary immersion systems for the mass propagation of sweet cherry cultivars and cherry rootstocks

T2 - development of a micropropagation procedure and effect of culture conditions on plant quality

AU - Godoy, Sebastián

AU - Tapia, Eduardo

AU - Seit, Paula

AU - Andrade, Diego

AU - Sánchez, Evelyn

AU - Andrade, Paola

AU - Almeida, Andréa Miyasaka

AU - Prieto, Humberto

PY - 2017/9/21

Y1 - 2017/9/21

N2 - Sweet cherry (Prunus avium L.) varieties and cherry rootstocks are an important part of the fruit industry, and difficulties associated with mass propagation provide an opportunity for the use of temporary immersion systems (TIS). We show the establishment of culture procedures for four genotypes: the rootstocks Maxma-14 and Colt and the varieties ‘Van’ and ‘Rainier.’ The starting explants were internodal segments from seedlings kept in solid propagation medium (PM) (Driver-Kuniyuki Walnut (DKW) base supplemented with indole butyric acid, benzyl amino purine; ascorbic acid, myo-inositol, and agar). Segments were cultured under TIS for 14 d and led to whole plant generation after 30 d of culturing in solid rooting media, which depended on whether they are varieties or rootstocks. A 15-d acclimatization phase led to establishment in greenhouse. The efficiency of TIS was specifically analyzed for the two best PM-derivative media and compared to cultures using solid medium. A number of shoots (Px), biomass (Qx), and sucrose consumption (SC) were evaluated for these purposes. The results showed that Maxma-14, Colt, and ‘Van’ TIS cultures had improved performance in comparison to solid cultures, whereas ‘Rainier’ showed no differences. The number of immersions influenced all of the productive parameters (Px, Qx, and SC), whereas genotype affected Px, and the time of immersion influenced SC. The best Qx and Px values were obtained with the rootstocks Maxma-14 and Colt, as well as the variety Van; these showed no hyperhydration. Physiological studies show that 14-d TIS-produced shoots represented an intermediate stage between solid-derived and adult plants, although the photosynthetic efficiencies of these materials revealed a lack of autotrophic ability at this point.

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