Temporal dynamics of genes involved in metabolic pathways of C and N of L. ferriphilum, in the industrial bioleaching process of escondida mine, Chile

Pedro A. Galleguillos, Visnja Music, Mauricio Acosta, Camila N. Salazar, Raquel Quatrini, Amir Shmaryahu, David S. Holmes, Antonio Velásquez, Cristian Espoz, Carlos Pinilla, Cecilia S. Demergasso

Resultado de la investigación: Conference contribution

9 Citas (Scopus)

Resumen

The structure of the microbial community inhabiting the copper bioleaching heap at Escondida mine has been systematically monitored since the operation was started up (2006), using biomolecular and microbiological analyses. Recent molecular analyses showed that L. ferriphilum was one of the most abundant organisms in the process during year 2012. In order to study the biological dynamics of carbon and nitrogen in the process, the expression levels of thirteen genes of L. ferriphilum were analyzed by RT-qPCR. The 16S rRNA and alaS genes were used as reference, and two relative quantification methods (ΔΔct and and Pffafl) were applied to estimate the relative expression levels of metabolic genes. On the day 110 of operation, a significant increment in the expression level of one gene involved in the cycle of tricarboxilic acids (2-oxoglutarate-acceptor oxidoreductase, oorA) was detected. By other hand, the expression level of two genes involved in carbohydrate metabolism (glgP, Glycogen phosphorilase, and glgA, Glycogen synthase) gradually increased, as the operation time progressed. The expression levels of genes involved in the fixation and assimilation of nitrogen increased at later stages of the process. A significant increase of the expression level of the gene annotated for Nitrogenase iron protein (nifH) was detected on the day 185 of operation. The opposite trend was observed for the gene annotated as Ammonium transporter protein (amt), as an elevated expression level was observed in earlier stages to suddenly decrease on the day 185 of operation, suggesting a change of the nitrogen source. In agreement with molecular quantitative analyses, this work confirmed that L. ferriphilum was an active member of the community during the period studied. This work gives new insights into biological dynamics of carbon and nitrogen, and suggests the potential guidelines to enhance the efficiency of biological components in industrial heap bioleaching processes.

Idioma originalEnglish
Título de la publicación alojadaIntegration of Scientific and Industrial Knowledge on Biohydrometallurgy
Páginas162-165
Número de páginas4
Volumen825
DOI
EstadoPublished - 2013
Evento20th International Biohydrometallurgy Symposium, IBS 2013 - Antofagasta, Chile
Duración: 8 oct 201311 oct 2013

Serie de la publicación

NombreAdvanced Materials Research
Volumen825
ISSN (versión impresa)10226680

Other

Other20th International Biohydrometallurgy Symposium, IBS 2013
PaísChile
CiudadAntofagasta
Período8/10/1311/10/13

Huella dactilar

Bioleaching
Genes
Nitrogen
Nitrogen fixation
Proteins
Carbon
Metabolic Networks and Pathways
Iron
Copper
Acids

ASJC Scopus subject areas

  • Engineering(all)

Citar esto

Galleguillos, P. A., Music, V., Acosta, M., Salazar, C. N., Quatrini, R., Shmaryahu, A., ... Demergasso, C. S. (2013). Temporal dynamics of genes involved in metabolic pathways of C and N of L. ferriphilum, in the industrial bioleaching process of escondida mine, Chile. En Integration of Scientific and Industrial Knowledge on Biohydrometallurgy (Vol. 825, pp. 162-165). (Advanced Materials Research; Vol. 825). https://doi.org/10.4028/www.scientific.net/AMR.825.162
Galleguillos, Pedro A. ; Music, Visnja ; Acosta, Mauricio ; Salazar, Camila N. ; Quatrini, Raquel ; Shmaryahu, Amir ; Holmes, David S. ; Velásquez, Antonio ; Espoz, Cristian ; Pinilla, Carlos ; Demergasso, Cecilia S. / Temporal dynamics of genes involved in metabolic pathways of C and N of L. ferriphilum, in the industrial bioleaching process of escondida mine, Chile. Integration of Scientific and Industrial Knowledge on Biohydrometallurgy. Vol. 825 2013. pp. 162-165 (Advanced Materials Research).
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abstract = "The structure of the microbial community inhabiting the copper bioleaching heap at Escondida mine has been systematically monitored since the operation was started up (2006), using biomolecular and microbiological analyses. Recent molecular analyses showed that L. ferriphilum was one of the most abundant organisms in the process during year 2012. In order to study the biological dynamics of carbon and nitrogen in the process, the expression levels of thirteen genes of L. ferriphilum were analyzed by RT-qPCR. The 16S rRNA and alaS genes were used as reference, and two relative quantification methods (ΔΔct and and Pffafl) were applied to estimate the relative expression levels of metabolic genes. On the day 110 of operation, a significant increment in the expression level of one gene involved in the cycle of tricarboxilic acids (2-oxoglutarate-acceptor oxidoreductase, oorA) was detected. By other hand, the expression level of two genes involved in carbohydrate metabolism (glgP, Glycogen phosphorilase, and glgA, Glycogen synthase) gradually increased, as the operation time progressed. The expression levels of genes involved in the fixation and assimilation of nitrogen increased at later stages of the process. A significant increase of the expression level of the gene annotated for Nitrogenase iron protein (nifH) was detected on the day 185 of operation. The opposite trend was observed for the gene annotated as Ammonium transporter protein (amt), as an elevated expression level was observed in earlier stages to suddenly decrease on the day 185 of operation, suggesting a change of the nitrogen source. In agreement with molecular quantitative analyses, this work confirmed that L. ferriphilum was an active member of the community during the period studied. This work gives new insights into biological dynamics of carbon and nitrogen, and suggests the potential guidelines to enhance the efficiency of biological components in industrial heap bioleaching processes.",
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Galleguillos, PA, Music, V, Acosta, M, Salazar, CN, Quatrini, R, Shmaryahu, A, Holmes, DS, Velásquez, A, Espoz, C, Pinilla, C & Demergasso, CS 2013, Temporal dynamics of genes involved in metabolic pathways of C and N of L. ferriphilum, in the industrial bioleaching process of escondida mine, Chile. En Integration of Scientific and Industrial Knowledge on Biohydrometallurgy. vol. 825, Advanced Materials Research, vol. 825, pp. 162-165, 20th International Biohydrometallurgy Symposium, IBS 2013, Antofagasta, Chile, 8/10/13. https://doi.org/10.4028/www.scientific.net/AMR.825.162

Temporal dynamics of genes involved in metabolic pathways of C and N of L. ferriphilum, in the industrial bioleaching process of escondida mine, Chile. / Galleguillos, Pedro A.; Music, Visnja; Acosta, Mauricio; Salazar, Camila N.; Quatrini, Raquel; Shmaryahu, Amir; Holmes, David S.; Velásquez, Antonio; Espoz, Cristian; Pinilla, Carlos; Demergasso, Cecilia S.

Integration of Scientific and Industrial Knowledge on Biohydrometallurgy. Vol. 825 2013. p. 162-165 (Advanced Materials Research; Vol. 825).

Resultado de la investigación: Conference contribution

TY - GEN

T1 - Temporal dynamics of genes involved in metabolic pathways of C and N of L. ferriphilum, in the industrial bioleaching process of escondida mine, Chile

AU - Galleguillos, Pedro A.

AU - Music, Visnja

AU - Acosta, Mauricio

AU - Salazar, Camila N.

AU - Quatrini, Raquel

AU - Shmaryahu, Amir

AU - Holmes, David S.

AU - Velásquez, Antonio

AU - Espoz, Cristian

AU - Pinilla, Carlos

AU - Demergasso, Cecilia S.

PY - 2013

Y1 - 2013

N2 - The structure of the microbial community inhabiting the copper bioleaching heap at Escondida mine has been systematically monitored since the operation was started up (2006), using biomolecular and microbiological analyses. Recent molecular analyses showed that L. ferriphilum was one of the most abundant organisms in the process during year 2012. In order to study the biological dynamics of carbon and nitrogen in the process, the expression levels of thirteen genes of L. ferriphilum were analyzed by RT-qPCR. The 16S rRNA and alaS genes were used as reference, and two relative quantification methods (ΔΔct and and Pffafl) were applied to estimate the relative expression levels of metabolic genes. On the day 110 of operation, a significant increment in the expression level of one gene involved in the cycle of tricarboxilic acids (2-oxoglutarate-acceptor oxidoreductase, oorA) was detected. By other hand, the expression level of two genes involved in carbohydrate metabolism (glgP, Glycogen phosphorilase, and glgA, Glycogen synthase) gradually increased, as the operation time progressed. The expression levels of genes involved in the fixation and assimilation of nitrogen increased at later stages of the process. A significant increase of the expression level of the gene annotated for Nitrogenase iron protein (nifH) was detected on the day 185 of operation. The opposite trend was observed for the gene annotated as Ammonium transporter protein (amt), as an elevated expression level was observed in earlier stages to suddenly decrease on the day 185 of operation, suggesting a change of the nitrogen source. In agreement with molecular quantitative analyses, this work confirmed that L. ferriphilum was an active member of the community during the period studied. This work gives new insights into biological dynamics of carbon and nitrogen, and suggests the potential guidelines to enhance the efficiency of biological components in industrial heap bioleaching processes.

AB - The structure of the microbial community inhabiting the copper bioleaching heap at Escondida mine has been systematically monitored since the operation was started up (2006), using biomolecular and microbiological analyses. Recent molecular analyses showed that L. ferriphilum was one of the most abundant organisms in the process during year 2012. In order to study the biological dynamics of carbon and nitrogen in the process, the expression levels of thirteen genes of L. ferriphilum were analyzed by RT-qPCR. The 16S rRNA and alaS genes were used as reference, and two relative quantification methods (ΔΔct and and Pffafl) were applied to estimate the relative expression levels of metabolic genes. On the day 110 of operation, a significant increment in the expression level of one gene involved in the cycle of tricarboxilic acids (2-oxoglutarate-acceptor oxidoreductase, oorA) was detected. By other hand, the expression level of two genes involved in carbohydrate metabolism (glgP, Glycogen phosphorilase, and glgA, Glycogen synthase) gradually increased, as the operation time progressed. The expression levels of genes involved in the fixation and assimilation of nitrogen increased at later stages of the process. A significant increase of the expression level of the gene annotated for Nitrogenase iron protein (nifH) was detected on the day 185 of operation. The opposite trend was observed for the gene annotated as Ammonium transporter protein (amt), as an elevated expression level was observed in earlier stages to suddenly decrease on the day 185 of operation, suggesting a change of the nitrogen source. In agreement with molecular quantitative analyses, this work confirmed that L. ferriphilum was an active member of the community during the period studied. This work gives new insights into biological dynamics of carbon and nitrogen, and suggests the potential guidelines to enhance the efficiency of biological components in industrial heap bioleaching processes.

KW - Bioleaching

KW - L. ferriphilum

KW - Temporal dynamics

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U2 - 10.4028/www.scientific.net/AMR.825.162

DO - 10.4028/www.scientific.net/AMR.825.162

M3 - Conference contribution

AN - SCOPUS:84886797277

SN - 9783037858912

VL - 825

T3 - Advanced Materials Research

SP - 162

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BT - Integration of Scientific and Industrial Knowledge on Biohydrometallurgy

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Galleguillos PA, Music V, Acosta M, Salazar CN, Quatrini R, Shmaryahu A y otros. Temporal dynamics of genes involved in metabolic pathways of C and N of L. ferriphilum, in the industrial bioleaching process of escondida mine, Chile. En Integration of Scientific and Industrial Knowledge on Biohydrometallurgy. Vol. 825. 2013. p. 162-165. (Advanced Materials Research). https://doi.org/10.4028/www.scientific.net/AMR.825.162