Tellurite enters Escherichia coli mainly through the PitA phosphate transporter

Alex O. Elías, María José Abarca, Rebecca A. Montes, Thomas G. Chasteen, José M. Pérez-Donoso, Claudio C. Vásquez

Resultado de la investigación: Article

15 Citas (Scopus)

Resumen

Several transporters suspected to be involved in tellurite uptake in Escherichia coli were analyzed. Results showed that the PitA phosphate transporter was related to tellurite uptake. Escherichia coli ΔpitA was approximately four-fold more tolerant to tellurite, and cell viability remained almost unchanged during prolonged exposure to the toxicant as compared with wild type or ΔpitB cells. Notably, reduced thiols (toxicant targets) as well as superoxide dismutase, catalase, and fumarase C activities did not change when exposing the ΔpitA strain to tellurite, suggesting that tellurite-triggered oxidative damage is attenuated in the absence of PitA. After toxicant exposure, remaining extracellular tellurite was higher in E. coli ΔpitA than in control cells. Whereas inductively coupled plasma atomic emission spectrometric studies confirmed that E. coli ΔpitA accumulates ~50% less tellurite than the other strains under study, tellurite strongly inhibited 32Pi uptake suggesting that the PitA transporter is one of the main responsible for tellurite uptake in this bacterium.

Idioma originalEnglish
Páginas (desde-hasta)259-267
Número de páginas9
PublicaciónMicrobiologyOpen
Volumen1
N.º3
DOI
EstadoPublished - sep 2012

Huella dactilar

Phosphate Transport Proteins
Escherichia coli
tellurous acid
Sulfhydryl Compounds
Catalase
Superoxide Dismutase
Cell Survival
Bacteria

ASJC Scopus subject areas

  • Microbiology

Citar esto

Elías, A. O., Abarca, M. J., Montes, R. A., Chasteen, T. G., Pérez-Donoso, J. M., & Vásquez, C. C. (2012). Tellurite enters Escherichia coli mainly through the PitA phosphate transporter. MicrobiologyOpen, 1(3), 259-267. https://doi.org/10.1002/mbo3.26
Elías, Alex O. ; Abarca, María José ; Montes, Rebecca A. ; Chasteen, Thomas G. ; Pérez-Donoso, José M. ; Vásquez, Claudio C. / Tellurite enters Escherichia coli mainly through the PitA phosphate transporter. En: MicrobiologyOpen. 2012 ; Vol. 1, N.º 3. pp. 259-267.
@article{9a7990d5d93a40ee87fbc2c09acbb0f3,
title = "Tellurite enters Escherichia coli mainly through the PitA phosphate transporter",
abstract = "Several transporters suspected to be involved in tellurite uptake in Escherichia coli were analyzed. Results showed that the PitA phosphate transporter was related to tellurite uptake. Escherichia coli ΔpitA was approximately four-fold more tolerant to tellurite, and cell viability remained almost unchanged during prolonged exposure to the toxicant as compared with wild type or ΔpitB cells. Notably, reduced thiols (toxicant targets) as well as superoxide dismutase, catalase, and fumarase C activities did not change when exposing the ΔpitA strain to tellurite, suggesting that tellurite-triggered oxidative damage is attenuated in the absence of PitA. After toxicant exposure, remaining extracellular tellurite was higher in E. coli ΔpitA than in control cells. Whereas inductively coupled plasma atomic emission spectrometric studies confirmed that E. coli ΔpitA accumulates ~50{\%} less tellurite than the other strains under study, tellurite strongly inhibited 32Pi uptake suggesting that the PitA transporter is one of the main responsible for tellurite uptake in this bacterium.",
keywords = "Escherichia coli, Phosphate transport, PitA, Tellurite uptake",
author = "El{\'i}as, {Alex O.} and Abarca, {Mar{\'i}a Jos{\'e}} and Montes, {Rebecca A.} and Chasteen, {Thomas G.} and P{\'e}rez-Donoso, {Jos{\'e} M.} and V{\'a}squez, {Claudio C.}",
year = "2012",
month = "9",
doi = "10.1002/mbo3.26",
language = "English",
volume = "1",
pages = "259--267",
journal = "MicrobiologyOpen",
issn = "2045-8827",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

Elías, AO, Abarca, MJ, Montes, RA, Chasteen, TG, Pérez-Donoso, JM & Vásquez, CC 2012, 'Tellurite enters Escherichia coli mainly through the PitA phosphate transporter', MicrobiologyOpen, vol. 1, n.º 3, pp. 259-267. https://doi.org/10.1002/mbo3.26

Tellurite enters Escherichia coli mainly through the PitA phosphate transporter. / Elías, Alex O.; Abarca, María José; Montes, Rebecca A.; Chasteen, Thomas G.; Pérez-Donoso, José M.; Vásquez, Claudio C.

En: MicrobiologyOpen, Vol. 1, N.º 3, 09.2012, p. 259-267.

Resultado de la investigación: Article

TY - JOUR

T1 - Tellurite enters Escherichia coli mainly through the PitA phosphate transporter

AU - Elías, Alex O.

AU - Abarca, María José

AU - Montes, Rebecca A.

AU - Chasteen, Thomas G.

AU - Pérez-Donoso, José M.

AU - Vásquez, Claudio C.

PY - 2012/9

Y1 - 2012/9

N2 - Several transporters suspected to be involved in tellurite uptake in Escherichia coli were analyzed. Results showed that the PitA phosphate transporter was related to tellurite uptake. Escherichia coli ΔpitA was approximately four-fold more tolerant to tellurite, and cell viability remained almost unchanged during prolonged exposure to the toxicant as compared with wild type or ΔpitB cells. Notably, reduced thiols (toxicant targets) as well as superoxide dismutase, catalase, and fumarase C activities did not change when exposing the ΔpitA strain to tellurite, suggesting that tellurite-triggered oxidative damage is attenuated in the absence of PitA. After toxicant exposure, remaining extracellular tellurite was higher in E. coli ΔpitA than in control cells. Whereas inductively coupled plasma atomic emission spectrometric studies confirmed that E. coli ΔpitA accumulates ~50% less tellurite than the other strains under study, tellurite strongly inhibited 32Pi uptake suggesting that the PitA transporter is one of the main responsible for tellurite uptake in this bacterium.

AB - Several transporters suspected to be involved in tellurite uptake in Escherichia coli were analyzed. Results showed that the PitA phosphate transporter was related to tellurite uptake. Escherichia coli ΔpitA was approximately four-fold more tolerant to tellurite, and cell viability remained almost unchanged during prolonged exposure to the toxicant as compared with wild type or ΔpitB cells. Notably, reduced thiols (toxicant targets) as well as superoxide dismutase, catalase, and fumarase C activities did not change when exposing the ΔpitA strain to tellurite, suggesting that tellurite-triggered oxidative damage is attenuated in the absence of PitA. After toxicant exposure, remaining extracellular tellurite was higher in E. coli ΔpitA than in control cells. Whereas inductively coupled plasma atomic emission spectrometric studies confirmed that E. coli ΔpitA accumulates ~50% less tellurite than the other strains under study, tellurite strongly inhibited 32Pi uptake suggesting that the PitA transporter is one of the main responsible for tellurite uptake in this bacterium.

KW - Escherichia coli

KW - Phosphate transport

KW - PitA

KW - Tellurite uptake

UR - http://www.scopus.com/inward/record.url?scp=84878020523&partnerID=8YFLogxK

U2 - 10.1002/mbo3.26

DO - 10.1002/mbo3.26

M3 - Article

C2 - 23189244

AN - SCOPUS:84878020523

VL - 1

SP - 259

EP - 267

JO - MicrobiologyOpen

JF - MicrobiologyOpen

SN - 2045-8827

IS - 3

ER -