Synthesis of hepatitis B surface and core antigens in E. coli

Jeffrey C. Edman, Robert A. Hallewell, Pablo Valenzuela, Howard M. Goodman, William J. Rutter

Resultado de la investigación: Article

97 Citas (Scopus)

Resumen

Hepatitis B virus (HBV) is the cause of a debilitating and potentially fatal disease, and possibly also of primary hepato-cellular carcinoma 1. A vaccine against hepatitis B would therefore be of considerable biomedical significance. One such vaccine, produced from the 22-nm particle form of hepatitis B surface antigen (HBsAg), has recently been found effective in clinical tests2, but being derived from the sera of chronic carriers, it is expensive and its supply limited. The application of recombinant DNA methods can, in principle, provide a limitless source of vaccine. HBV DNA has been cloned in bacteria and the genes coding for HBsAg and hepatitis B core antigen (HBcAg) identified3-5. Small amounts of both antigens have been synthesized in Escherichia coli3,6,7 and to enhance this synthesis we have constructed plasmids capable of expressing the genes for the antigens under the control of the efficient tryptophan (trp) operon regulatory region8. We describe here the construction of such recombinant plasmids which direct the synthesis of high levels of HBcAg and a β-lactamase: HBsAg fusion polypeptide.

Idioma originalEnglish
Páginas (desde-hasta)503-506
Número de páginas4
PublicaciónNature
Volumen291
N.º5815
DOI
EstadoPublished - 1 dic 1981

Huella dactilar

Hepatitis B Core Antigens
Hepatitis B Surface Antigens
Escherichia coli
Hepatitis B virus
Plasmids
Vaccines
Antigens
Escherichia
Recombinant DNA
Operon
Tryptophan
Genes
Bacteria
Carcinoma
Peptides
DNA
Serum

ASJC Scopus subject areas

  • General

Citar esto

Edman, J. C., Hallewell, R. A., Valenzuela, P., Goodman, H. M., & Rutter, W. J. (1981). Synthesis of hepatitis B surface and core antigens in E. coli. Nature, 291(5815), 503-506. https://doi.org/10.1038/291503a0
Edman, Jeffrey C. ; Hallewell, Robert A. ; Valenzuela, Pablo ; Goodman, Howard M. ; Rutter, William J. / Synthesis of hepatitis B surface and core antigens in E. coli. En: Nature. 1981 ; Vol. 291, N.º 5815. pp. 503-506.
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Edman, JC, Hallewell, RA, Valenzuela, P, Goodman, HM & Rutter, WJ 1981, 'Synthesis of hepatitis B surface and core antigens in E. coli', Nature, vol. 291, n.º 5815, pp. 503-506. https://doi.org/10.1038/291503a0

Synthesis of hepatitis B surface and core antigens in E. coli. / Edman, Jeffrey C.; Hallewell, Robert A.; Valenzuela, Pablo; Goodman, Howard M.; Rutter, William J.

En: Nature, Vol. 291, N.º 5815, 01.12.1981, p. 503-506.

Resultado de la investigación: Article

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Edman JC, Hallewell RA, Valenzuela P, Goodman HM, Rutter WJ. Synthesis of hepatitis B surface and core antigens in E. coli. Nature. 1981 dic 1;291(5815):503-506. https://doi.org/10.1038/291503a0