Sugar metabolism and developmental stages of rubber tree (Hevea brasiliensis L.) seeds

Genaina Aparecida de Souza, Denise Cunha Fernandes dos Santos Dias, Thaline Martins Pimenta, Andrea Lanna Almeida, Edgard Augusto de Toledo Picoli, Antônio de Pádua Alvarenga, José Cleydson Ferreira da Silva

Resultado de la investigación: Article

4 Citas (Scopus)

Resumen

Changes in the concentration of sugars and sucrose metabolism enzymes can characterize the developmental stages of a seed. In recalcitrant species such as Hevea brasiliensis L., little is known about these changes. We aimed to evaluate the three main stages of development of rubber tree seeds – histodifferentiation, cell elongation and accumulation of reserves. The activities of acid and neutral invertases (E.C. 3.2.1.26) and sucrose synthase (EC 2.4.1.13), and the concentrations of reducing sugars (RS), total soluble sugars (TSS) and sucrose (Suc) were determined concomitantly with the histochemical and anatomical evaluation of seed structure. Histodifferentiation in rubber tree seeds occurs up to 75 days after anthesis (DAA). The concentration of RS is high and of Suc is low during seed histodifferentiation, which occurs along with a visible increase in the number of cell divisions. After that period, there is an increase in the concentration of Suc (mg g−1) and in the number and size of starch granules, and a decrease in the concentration of RS (mg g−1). At that point, cell elongation occurs. At 135 DAA, there is an inversion in the concentration of these two sugars and an increase in reserve accumulation. Thus, in seeds of the evaluated clone, the period up to 75 DAA is characterized as the histodifferentiation stage, while from that time up to 120 DAA the cell elongation stage takes place. The final stage of seed maturation and reserve accumulation begins at 135 DAA, and the seed, including the embryo, is completely formed at 175 DAA.

Idioma originalEnglish
Páginas (desde-hasta)495-505
Número de páginas11
PublicaciónPhysiologia Plantarum
Volumen162
N.º4
DOI
EstadoPublished - 1 abr 2018

Huella dactilar

Hevea
Hevea brasiliensis
Seeds
developmental stages
flowering
sugars
metabolism
seeds
Sucrose
reducing sugars
sucrose
cell growth
sucrose synthase
beta-fructofuranosidase
seed maturation
beta-Fructofuranosidase
starch granules
embryo (plant)
cell division
Cell Division

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Citar esto

de Souza, G. A., dos Santos Dias, D. C. F., Pimenta, T. M., Almeida, A. L., de Toledo Picoli, E. A., de Pádua Alvarenga, A., & da Silva, J. C. F. (2018). Sugar metabolism and developmental stages of rubber tree (Hevea brasiliensis L.) seeds. Physiologia Plantarum, 162(4), 495-505. https://doi.org/10.1111/ppl.12650
de Souza, Genaina Aparecida ; dos Santos Dias, Denise Cunha Fernandes ; Pimenta, Thaline Martins ; Almeida, Andrea Lanna ; de Toledo Picoli, Edgard Augusto ; de Pádua Alvarenga, Antônio ; da Silva, José Cleydson Ferreira. / Sugar metabolism and developmental stages of rubber tree (Hevea brasiliensis L.) seeds. En: Physiologia Plantarum. 2018 ; Vol. 162, N.º 4. pp. 495-505.
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abstract = "Changes in the concentration of sugars and sucrose metabolism enzymes can characterize the developmental stages of a seed. In recalcitrant species such as Hevea brasiliensis L., little is known about these changes. We aimed to evaluate the three main stages of development of rubber tree seeds – histodifferentiation, cell elongation and accumulation of reserves. The activities of acid and neutral invertases (E.C. 3.2.1.26) and sucrose synthase (EC 2.4.1.13), and the concentrations of reducing sugars (RS), total soluble sugars (TSS) and sucrose (Suc) were determined concomitantly with the histochemical and anatomical evaluation of seed structure. Histodifferentiation in rubber tree seeds occurs up to 75 days after anthesis (DAA). The concentration of RS is high and of Suc is low during seed histodifferentiation, which occurs along with a visible increase in the number of cell divisions. After that period, there is an increase in the concentration of Suc (mg g−1) and in the number and size of starch granules, and a decrease in the concentration of RS (mg g−1). At that point, cell elongation occurs. At 135 DAA, there is an inversion in the concentration of these two sugars and an increase in reserve accumulation. Thus, in seeds of the evaluated clone, the period up to 75 DAA is characterized as the histodifferentiation stage, while from that time up to 120 DAA the cell elongation stage takes place. The final stage of seed maturation and reserve accumulation begins at 135 DAA, and the seed, including the embryo, is completely formed at 175 DAA.",
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de Souza, GA, dos Santos Dias, DCF, Pimenta, TM, Almeida, AL, de Toledo Picoli, EA, de Pádua Alvarenga, A & da Silva, JCF 2018, 'Sugar metabolism and developmental stages of rubber tree (Hevea brasiliensis L.) seeds', Physiologia Plantarum, vol. 162, n.º 4, pp. 495-505. https://doi.org/10.1111/ppl.12650

Sugar metabolism and developmental stages of rubber tree (Hevea brasiliensis L.) seeds. / de Souza, Genaina Aparecida; dos Santos Dias, Denise Cunha Fernandes; Pimenta, Thaline Martins; Almeida, Andrea Lanna; de Toledo Picoli, Edgard Augusto; de Pádua Alvarenga, Antônio; da Silva, José Cleydson Ferreira.

En: Physiologia Plantarum, Vol. 162, N.º 4, 01.04.2018, p. 495-505.

Resultado de la investigación: Article

TY - JOUR

T1 - Sugar metabolism and developmental stages of rubber tree (Hevea brasiliensis L.) seeds

AU - de Souza, Genaina Aparecida

AU - dos Santos Dias, Denise Cunha Fernandes

AU - Pimenta, Thaline Martins

AU - Almeida, Andrea Lanna

AU - de Toledo Picoli, Edgard Augusto

AU - de Pádua Alvarenga, Antônio

AU - da Silva, José Cleydson Ferreira

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Changes in the concentration of sugars and sucrose metabolism enzymes can characterize the developmental stages of a seed. In recalcitrant species such as Hevea brasiliensis L., little is known about these changes. We aimed to evaluate the three main stages of development of rubber tree seeds – histodifferentiation, cell elongation and accumulation of reserves. The activities of acid and neutral invertases (E.C. 3.2.1.26) and sucrose synthase (EC 2.4.1.13), and the concentrations of reducing sugars (RS), total soluble sugars (TSS) and sucrose (Suc) were determined concomitantly with the histochemical and anatomical evaluation of seed structure. Histodifferentiation in rubber tree seeds occurs up to 75 days after anthesis (DAA). The concentration of RS is high and of Suc is low during seed histodifferentiation, which occurs along with a visible increase in the number of cell divisions. After that period, there is an increase in the concentration of Suc (mg g−1) and in the number and size of starch granules, and a decrease in the concentration of RS (mg g−1). At that point, cell elongation occurs. At 135 DAA, there is an inversion in the concentration of these two sugars and an increase in reserve accumulation. Thus, in seeds of the evaluated clone, the period up to 75 DAA is characterized as the histodifferentiation stage, while from that time up to 120 DAA the cell elongation stage takes place. The final stage of seed maturation and reserve accumulation begins at 135 DAA, and the seed, including the embryo, is completely formed at 175 DAA.

AB - Changes in the concentration of sugars and sucrose metabolism enzymes can characterize the developmental stages of a seed. In recalcitrant species such as Hevea brasiliensis L., little is known about these changes. We aimed to evaluate the three main stages of development of rubber tree seeds – histodifferentiation, cell elongation and accumulation of reserves. The activities of acid and neutral invertases (E.C. 3.2.1.26) and sucrose synthase (EC 2.4.1.13), and the concentrations of reducing sugars (RS), total soluble sugars (TSS) and sucrose (Suc) were determined concomitantly with the histochemical and anatomical evaluation of seed structure. Histodifferentiation in rubber tree seeds occurs up to 75 days after anthesis (DAA). The concentration of RS is high and of Suc is low during seed histodifferentiation, which occurs along with a visible increase in the number of cell divisions. After that period, there is an increase in the concentration of Suc (mg g−1) and in the number and size of starch granules, and a decrease in the concentration of RS (mg g−1). At that point, cell elongation occurs. At 135 DAA, there is an inversion in the concentration of these two sugars and an increase in reserve accumulation. Thus, in seeds of the evaluated clone, the period up to 75 DAA is characterized as the histodifferentiation stage, while from that time up to 120 DAA the cell elongation stage takes place. The final stage of seed maturation and reserve accumulation begins at 135 DAA, and the seed, including the embryo, is completely formed at 175 DAA.

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de Souza GA, dos Santos Dias DCF, Pimenta TM, Almeida AL, de Toledo Picoli EA, de Pádua Alvarenga A y otros. Sugar metabolism and developmental stages of rubber tree (Hevea brasiliensis L.) seeds. Physiologia Plantarum. 2018 abr 1;162(4):495-505. https://doi.org/10.1111/ppl.12650